The Mechanism Study of Gonadal Differentiation in Hermaphroditic Fish

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基本資料

Project title

Code/計畫編號

MOST104-2321-B019-006-MY3

Translated Name/計畫中文名

魚類雌雄同體兩性性腺分化發育的機制研究(兩岸合作研究)

Project Coordinator/計畫主持人

Guan-Chung Wu

Funding Organization/主管機關

National Science and Technology Council

Co-Investigator(s)/共同執行人

鄭絢如
張清風

Department/Unit

Department of Aquaculture

Website

https://www.grb.gov.tw/search/planDetail?id=12023580

Year

2017

Start date/計畫起

01-01-2017

Expected Completion/計畫迄

31-12-2017

Bugetid/研究經費

1800千元

ResearchField/研究領域

漁業

"雌雄同體魚類沒有共同的祖先而且具有不同的性別決定策略。因此魚類從雌雄異體往雌雄同體演化的過渡階段是如何適應錯誤的生理環境？機制仍然未知。鰻魚的生殖腺分化具有幼齡雌雄同體的現象。生殖腺先分化為類似卵巢(Syrski organ)的結構，雌性性別決定則繼續發育為卵巢；雄性性別決定後，則是從類似卵巢的結構中進行精巢的分化與新生。因此本計畫將利用鰻魚作為模式，探討幼齡雌雄同體(潛在的雌雄異體→雌雄同體的演化過度狀態)鰻魚的性別決定與生殖腺分化機制。因此我們提出3 個問題： (1) 鰻魚性別決定相關因子對雌性的作用是否較雄性更直接。 (2) 性別決定因子是否影響腦和生殖腺中環化酵素的表觀遺傳差異性。 (3) 雌性生理環境的弱化是否會直接造成雄性精巢的發育。本計畫將探討鰻魚生殖腺兩性並存的過渡階段，腦-腦下垂體分泌因子如何調控生殖腺的生理環境(雄性分化發育與雌性退化)。我們將經由6 個重要的實驗探討這個問題。經由本計畫的研究(包括中科院水生所的共同研究)，我們預期可以進一步解答雌雄同體演化的過渡階段，腦-腦下垂體分泌因子如何調控生殖腺的生理環境，對於雌雄同體魚類的演化機制及性別調控提供至為重要之理論與應用。亦可做為未來鰻魚雌性選育的參考。" "No ancient ancestry and no single sex-determining mechanism are involved in the hermaphroditic fishes. Thus, how the presence of more than one sex at a time existed during an evolutionary transition from gonochorism to hermaphroditism in fish remains unknown. Therefore, this project will follow to our understanding of the interaction between maleness and femaleness in the hermaphroditic fish. Mature eels (Anguilla) are gonochoristic species that begin development with an ambiguous gonad. Male development via a transitional stage where the gonads contain both sexual germlines (Syrski organ), whereas females develop ovaries directly from the differentiated primordial gonad. Thus, this project will use the eel (juvenile hermaphrodite) as a model to understand the mechanism of sex determination and gonadal differentiation during the transient stage of the potential hermaphroditism. Thus, we have 3 mainly questions to ask: (1) Do sex determining factors have more influence in female than in male? (2) Have any epigenetic (DNA methylation levels) difference in the brain and gonad when fish that stay in different environment (sex determining-related factors)? (3) Can fish induce maleness by inhibiting female micro-environment? This project will focus on the sex determination of brain-pituitary of the possible mechanism for the inhibiting femaleness (or stimulating maleness) at a time during a transient stage of juvenile hermaphroditic eel. The experiments will include as follows: (1) The expression profile of sex-specific genes and the sensitivity for brain-pituitary releasing factors in the maleness and femaleness. (2) The epigenetic control (DNA methylation levels) of genome and cyp19a1a promoter in the brain and gonad of both genders. (3) The fate of maleness is influenced by the competition with femaleness. These results will expect to shed light on the reason how the environment condition change can alter the fate of bisexual gonad from one sex to the other sex in the hermaphroditic fish. Our results will have great impact and contribution to the sex differentiation and sex change in basic academic and applied aspects."

Keyword(s)

雌雄同體魚類
性別決定
生殖腺分化
環化酵素
表觀遺傳
hermaphroditic fish
sex determination
gonadal differentiation
aromatase
epigenetic control

DSpace CRIS

The Mechanism Study of Gonadal Differentiation in Hermaphroditic Fish

基本資料

Description