Development of Rapid Detection Kit in Taura Syndrome Virus in Shrimp (I)

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簡歷

基本資料

Project title

Code/計畫編號

MOST103-2622-B019-002-CC2

Translated Name/計畫中文名

開發蝦類桃拉病毒的快速檢測套組( I )

Project Coordinator/計畫主持人

Ming-Wei Lu

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Aquaculture

Website

https://www.grb.gov.tw/search/planDetail?id=8399078

Year

2014

Start date/計畫起

01-11-2014

Expected Completion/計畫迄

31-10-2015

Bugetid/研究經費

1000千元

ResearchField/研究領域

漁業

"白蝦(Pacific white shrimp, Litopenaeus vannamei)肉質鮮美且富含高蛋白質，加以具有生長快速的優勢，成為台灣養殖產業上重要的經濟物種。但病毒性疾病的爆發一直是白蝦養殖的限制因子。桃拉病毒 (Taura syndrome virus, TSV)是其中一個造成白蝦大量死亡的病毒性病原體，有高達40-90%的死亡率。目前市面上通用的檢測方式多半靈敏度較低、花費時間較長或操作不便，因此我們預計研發一套快速、靈敏、方便操作的蝦類桃拉病毒檢測套組。第一年預計生產桃拉病毒抗體，應用磁減量技術，將磁珠結合桃拉病毒抗體，偵測病毒結合抗體後的磁訊號轉變，建立一個高敏感度、快速方便的磁減量檢測機台。第二年預計開發一套快速萃取套組搭配磁減量檢測系統，並建立一套標準作業流程，將此平台商品化準備上市。此平台監測系統也可套用在其他蝦類病毒檢測上，搭配過去與磁量生技共同研發之神經壞死病毒與虹彩病毒檢測套組，建立全面性之水產養殖監測系統。 " "Pacific white shrimp (Litopenaeus vannamei) has becomes economically high-valued aquaculture species in Taiwan due to advantages such as great quality of flesh and fast growing rate. However, the outbreaks of viral diseases become hindrance to the development of this industry. Taura syndrome virus (TSV) is one of critical viral disease in Pacific white shrimp, caused 40-90% mortality. Although the commercial detection test kit is available, there are rooms for improvements in sensitivity, easy operation and etc. In this project, a fast, high-sensitive and easy of operation magnetic immunoassay analyzed kit will be developed. In first year, the aim is to produce the specific antibody against TSV, developing a novel detection platform which can detect the TSV titer. In second year, the aim is to develop a fast viral extraction kit. Otherwise, we will also be standardized the operation procedure. Furthermore, it can be also detect other shrimp viral disease, we wish this viral detection platform can be used in the aquaculture industry and enhance the productivity."

DSpace CRIS

Development of Rapid Detection Kit in Taura Syndrome Virus in Shrimp (I)

基本資料

Description