Effects of Global Change on the Distribution and Nitrogen Status of Coccolithophores in the Seas Surrounding Taiwan in the Northwest Pacific

瀏覽統計 Email 通知 RSS Feed

簡歷

基本資料

Project title

Code/計畫編號

NSC102-2611-M019-021-MY2

Translated Name/計畫中文名

全球變遷對西北太平洋臺灣海域海洋生物地球化學與生態系統影響之長期觀測與研究(I)－子計畫：全球變遷因子對東海及西北太平洋黑潮海域鈣板藻生態與氮營養鹽限制之影響

Project Coordinator/計畫主持人

Lee-Kuo Kang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Institute of Marine Environment and Ecology

Website

https://www.grb.gov.tw/search/planDetail?id=8122874

Year

2014

Start date/計畫起

01-08-2014

Expected Completion/計畫迄

31-07-2015

Bugetid/研究經費

1092千元

ResearchField/研究領域

海洋科學

本申請案為全球變遷對西北太平洋臺灣海域海洋生物地球化學與生態系統影響之長期觀測與研究(一) 整合型計劃中的子計劃。由於鈣板藻在海洋碳循環及硫循環中均扮演重要的角色，其生長狀況與及在浮游植物群聚中的豐寡影響海洋初級生產力、海洋碳酸系統及海氣二氧化碳交換等，是探討全球環境變遷的議題上不可或缺的重要組成。然而在海洋中鈣板藻的生長經常被認為受到氮營養鹽所控制，所以申請人擬利用未來兩年以探討鈣板藻在台灣周邊海域的分布情形，並評估其在海洋中氮鹽利用情形。有鑑鈣板藻體型小，因此將採用合併傳統技術和分子生物技術的方法進行，包括使用偏光顯微鏡及螢光原位雜合反應來提高鈣板藻種類及定量上辨識的準確度、與及運用硝酸鹽運輸蛋白基因作為氮指標基因用於評估海洋中鈣板藻不同氮鹽之利用情形。預期結果可以讓我們瞭解鈣板藻在台灣周邊海域中的生態地位，而氮指標基因的應用則讓我們更有把握的判定鈣板藻是否受到氮鹽的控制。此外本計畫結果也能密切配合其他子計畫，共同瞭解現今海洋的生態狀況及其與海洋碳酸系統、海氣二氧化碳交換之關係等，進而有助於瞭解海洋生態系統在環境變遷的衝擊下如何做出反應。This proposal is the branch project in the integrated research project "Effects of Global Change on Ocean Biogeochemistry and Ecosystems in the Seas surrounding Taiwan in the Northwest Pacific -I" (ECOBEST-I). Coccolithophores, which are considered as the most productive calcifying organisms on earth, play an important role in the marine carbon and sulfur cycles. Since the formation of calcite skeletons in the surface layer and their subsequent sinking to depth modifies upper-ocean alkalinity and directly affects air/sea CO2 exchange, their growth and distribution have been implicated as important in response to the impact of climate change in the ocean. One of the key environmental factors thought to limit coccolithophores success is nitrogen, the proposed research plans to investigate the distribution of coccolithophores in the seas surrounding Taiwan and their nitrogen-related physiological status in a period of 2 years. Due to their small size, molecular techniques will be applied in this study. A powerful and efficient method combined polarized optical microscopy and fluorescent in situ hybridization (COD-FISH) will be used to investigate the abundance and species composition of coccolithophores. On the other hand, the expression of nitrate transporter genes will be applied to evaluate the nitrogen deficiencies of coccolithophores in the ocean. If granted, this proposal will not only bring new techniques of molecular biology to the filed coccolithophores ecology, but also work collaboratively with other projects under ECOBEST I to understand the responses of marine ecosystem and biogeochemistry under the impact of environmental changes.

Keyword(s)

鈣板藻
偏光顯微-螢光原位雜合法
硝酸運輸蛋白基因
氮限制指標基因
西北太平洋
coccolithophore
COD-FISH (Combined CaCO3 optical detection with fluorescent in situ hybridization)
nitrate transporter gene
nitrogen marker gene
Northwest Pacific

DSpace CRIS

Effects of Global Change on the Distribution and Nitrogen Status of Coccolithophores in the Seas Surrounding Taiwan in the Northwest Pacific

基本資料

Description