Plankton Ecological Processes in Subtropical-Shelf Ecosystems: Phytoplankton and Inorganic Nitrogen Uptake in the East China Sea(I)

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基本資料

Project title

Code/計畫編號

MOST107-2611-M019-009

Translated Name/計畫中文名

副熱帶陸棚浮游生物食物網生態過程研究-東海浮游植物對無機氮鹽的吸收特性分析(I)

Project Coordinator/計畫主持人

Lee-Kuo Kang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Institute of Marine Environment and Ecology

Website

https://www.grb.gov.tw/search/planDetail?id=12671119

Year

2018

Start date/計畫起

01-08-2018

Expected Completion/計畫迄

31-07-2019

Bugetid/研究經費

1294千元

ResearchField/研究領域

海洋科學

本申請案為副熱帶陸棚浮游生物食物網生態過程研究 (PEPSE) 整合型計劃中的子計劃之一。微生物生態圈中之單細胞微生物包括細菌、古生菌與真核微生物在海洋生物地球化學循環中扮演重要角色，是海洋生態系中重要的調控者。其中海洋氮循環的過程中更與微生物密不可分，當海洋中氮環境的改變也直接影響到微生物圈的群聚組成及豐度，因此了解浮游微生物對於無機氮鹽的吸收是為了解它們在生態系中功能的重要基礎資訊。在本計畫中主要以氮穩定性同位素追蹤方法來獲取浮游植物對於無機氮鹽吸收動力學的相關特性，並藉此了解在浮游植物中硝酸運輸基因表現與其對硝酸吸收能力間的關係，也有助於持續發展以硝酸運輸基因做為評估浮游植物氮利用情形的指標基因。另一方面會將相同的策略應用在海上實測部分，用來了解不同體型浮游植物對於無機氮鹽的吸收特性，配合偵測氮指標基因之表現情形，用來評估不同體型大小浮游植物在東海的分布情形及與其環境中氮源利用情形。預期這兩種方法的結合，不僅提供團隊可在海洋環境中評估微生物圈中氮營養鹽的利用情形外，也可與其他子計劃結果共同討論環境變遷對於微生物圈的影響。This proposal is the branch project in the integrated research project " Plankton Ecological Processes in Subtropical-shelf Ecosystems" (PEPSE). Microbes, which include bacteria, archaea, and protists, drive important biogeochemical cycles in the ocean. Marine nitrogen cycle is composed of multiple transformations of nitrogenous compounds, catalyzed primarily by microbes. With anthropogenic activities altering total nutrient loads to the ocean, it has changing the community structures of microorganisms in the ocean. Therefore, it is important to understand the adaptations of different functional groups of microorganisms to varying N forms. In this proposal, I plan to use 15N-tracer technique to characterize the nitrate uptake in Skeletonema in order to reveal its nitrate uptake ability with the regulation of nitrate transporter genes. This strategy will also be applied to field experiments to evaluate the nitrogen uptake and nitrogen status among different size-fractionated natural microplankton assemblages. This project will not only provide direct information to evaluate the nitrogen status in marine phytoplankton, but also contribute to explain the changes in microorganism community composition in response to environmental changes with other branch projects under the PEPSE.

Keyword(s)

浮游植物
硝酸運輸蛋白基因
氮穩定性同位素
氮吸收動力學
分子指標
phytoplankton
nitrate transporter gene (Nrt2)
nitrogen uptake kinetics
15N-tracer technique
nitrogen stable isotopes
molecular marker

DSpace CRIS

Plankton Ecological Processes in Subtropical-Shelf Ecosystems: Phytoplankton and Inorganic Nitrogen Uptake in the East China Sea(I)

基本資料

Description