Role of Crh in Endocrine Control of Acclimation to Salinity in Fish

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Project title

Code/計畫編號

NSC96-2313-B019-005-MY3

Translated Name/計畫中文名

魚類適應鹽度生理機制之新觀點-魚類適應鹽度過程內分泌調控---CRH的角色

Project Coordinator/計畫主持人

Ching-Fong Chang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Aquaculture

Website

https://www.grb.gov.tw/search/planDetail?id=1595475

Year

2008

Start date/計畫起

01-08-2008

Expected Completion/計畫迄

31-07-2009

Bugetid/研究經費

1100千元

ResearchField/研究領域

漁業

" 本研究過去針對魚類鰓、腸子與腎臟的cortisol receptor，prolactin receptor 及growth hormone receptor 的表現與腦下垂體prolactin 與growth hormone 激素的表現在不同鹽度環境滲透壓調節的關係，最近我們初步研究發現CRH-CRH receptor 不僅在下視丘─腦下垂體表現，且在魚鰓亦有生理反應性的表現，而CRH-CRH receptor 是緊迫生理反應包括能量代謝，免疫機能與滲透壓調控作用之重要內分泌系統。因此本研究擬以吳郭魚及黑鯛探討有關鰓CRH-CRH receptor 在魚類鹽度滲透壓適應的生理作用為何及鰓 CRH-CRH receptor 與傳統的下視丘─腦下垂體CRH-CRH receptor 之間的關係為何？本研究擬以三年期間進行下列之實驗： 1) 比較長期適應於海水與淡水吳郭魚腦-下視丘-腦下垂體及鰓所含CRH-CRH receptor mRNA 之表現。 2) 比較黑鯛經長期間適應於海水與淡水後，黑鯛腦、腦下垂體及鰓絲CRH 與CRH receptor 基因表現及Na+/K+-ATPase 活性之關係。 3) 魚類先經長期適應於淡水（吳郭魚）、海水（黑鯛）後，再短期急迫轉換至海水與淡水，在其適應過程中（海水→淡水；淡水→海水），鰓絲、腦與腦下垂體CRH 與CRH receptor 基因含量之變化及免疫（interleukin I）與緊迫（hsp 70）之反應。 4) 埋植cortisol 對吳郭魚及黑鯛下視丘及鰓CRH-CRH receptor 基因表現之影響。 5) 以離體培養鰓的技術，探討CRH 對黑鯛與吳郭魚鰓絲CRH 與CRH receptor mRNA 表現之影響 6) 以離體培養鰓的技術，探討cortisol 對黑鯛與吳郭魚鰓絲CRH 與CRH receptor mRNA 表現之影響。 7) 以in situ hybridization 方法，確定吳郭魚與黑鯛鰓CRH 的表現細胞，並與 mitochondria-rich cell（MR cell）細胞比較。 8) 以抗體免疫染色探討吳郭魚與黑鯛鰓表現CRH receptor 的細胞，並與MR cell 比較。 " " Our recent preliminary studies indicated that gill as well as hypothalamus-pituitary could significantly express corticotrophin-releasing hormone (CRH) – CRH receptor. The physiological roles of gill CRH-CRH receptor-cortisol receptor in the acclimation of environmental salinity and the relationship between gill and hypothalamus-pituitary CRH-CRH receptor have been the interesting and important objectives of these studies in euryhaline tilapia (freshwater fish) and black porgy (seawater fish). The following experiments will be conducted during 3-year-period. 1) The expression of gill and hypothalamus-pituitary CRH-CRH receptor transcripts and gill Na+/ K+-ATPase activity in the freshwater and seawater tilapia. 2) The expression of gill and hypothalamus-pituitary CRH-CRH receptor transcripts and gill Na+/K+ ATPase activity in freshwater and seawater black porgy. 3) The expression of gill and hypothalamus-pituitary CRH-CRH receptor transcripts and gill Na+/ K+-ATPase activity in the acute change of salinity in tilapia (freshwater→ seawater) and black porgy (seawater→freshwater). 4) The in vivo cortisol effects in the expression of gill and hypothalamus-pituitary CRH-CRH receptor transcripts in tilapia and black porgy. 5) The in vitro CRH effects in gill CRH and CRH receptor transcripts in black porgy and tilapia. 6) The in vitro cortisol effects in gill CRH and CRH receptor transcripts in black porgy and tilapia. 7) The localization of CRH cell in gill of black porgy and tilapia by using in situ hybridization. 8) The comparison of CRH receptor and mitochondria-rich cell by the immunochemical staining against CRH receptor and Na+/ K+-ATPase antibody. "

Keyword(s)

黑鯛
吳郭魚
滲透壓調控作用
CRH
CRH 受體
鰓
Black porgy
tilapia
osmoregulation
gill
CRH receptors

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Role of Crh in Endocrine Control of Acclimation to Salinity in Fish

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