Development and application of agricultural by product as immunomodulation compound on Taiwan tilapia

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Project title

Code/計畫編號

108農科-14.3.1-科-a4

Translated Name/計畫中文名

優質臺灣鯛種苗培育、養殖管理及副產品跨域加值

Project Coordinator/計畫主持人

Meng-Chou Lee

Funding Organization/主管機關

Council of Agriculture,Executive Yuan

Co-Investigator(s)/共同執行人

李柏蒼
吳育昇
張富傑
洪聖雯
趙貞雅
葉翰揚
蔡秉諺
陳黃兆君
賴玫伊
周立進
劉梓軒
黎敬凌

Department/Unit

Department of Aquaculture

Website

https://www.grb.gov.tw/search/planDetail?id=13230601

Year

2019

Start date/計畫起

01-01-2019

Expected Completion/計畫迄

31-12-2019

Bugetid/研究經費

2000千元

ResearchField/研究領域

漁業

本研究探討農業副產物如靈芝 (Ganoderma lucidum)與巴西蘑菇 (Agaricus blazei)生長穀糧基質及海木耳 (Sarcodia suiae)作為臺灣鯛免疫調節劑之可行性分析。在體外實驗中，除最高濃度 (1000μg/ml)外，三種調節物之水萃物對肝、脾臟細胞皆無毒性。基因檢測結果發現，以低溫萃取之海木耳水萃物 (SSE) 可顯著提升臺灣鯛頭腎初代細胞免疫基因，如TP2、TP4、IL-8、IL-10、IL-12b 及TLR5，此情形在臺灣鯛頭腎細胞株 (Tilapia head kidney, THK) 有相似之結果，但高溫萃取之海木耳水萃物則無此效應。此外，巴西蘑菇生長基質水萃物處理之巨噬細胞有較好之吞噬活性，因此挑選海木耳與巴西蘑菇生長基質作為後續實驗之材料。以高、低溫萃取之ABGME、SSE或兩者混合之複方 (ABGME+SSE, A+S)處理臺灣鯛細胞，低溫方式獲得之萃取物仍具有較佳之免疫刺激效應，且複方組有免疫刺激加成之趨勢，能夠顯著提升初代頭腎細胞及THK之免疫基因，亦能誘發肝、脾臟巨噬細胞之吞噬活性。在投餵實驗中，連續投餵臺灣鯛1、3、7、14及28天含有不同比例 (1%、2.5%和 5%)之巴西蘑菇穀糧基質 (ABGM)或海木耳藻粉 (SS)飼料後，以無乳鏈球菌 ( Streptococcus agalactiae)進行腹腔注射攻擊試驗。結果顯示，比較各比例與連續投餵天數，連續投餵臺灣鯛7天含有1%巴西蘑菇穀糧基質 (ABGM)或1%海木耳 (SS)之飼料；連續投餵台灣鯛28天含有5%巴西蘑菇穀糧基質(ABGM)或1%海木耳(SS)飼料有較佳之保護效果。This study investigated the feasibility of agricultural byproducts such as growing substrate of Ganoderma lucidum and Agaricus blazei, and Sarcodia suiae as immunomodulators for Taiwan tilapia. In in vitro study, except for the highest concentration (1000 μg/ml), the aqueous extracts of the three regulators were not toxic to liver and spleen cells. Furthermore, gene expression analyses showed that low (but not high) temperature water extracts, of S. suiae (SSE) can induce the expression of immune related genes in primary head kidney cells, such as TP2, TP4, IL-8, IL-10, IL-12b and TLR5. Similar results were obtained in Tilapia head kidney (THK) cells. In comparison of immune gene modulating effect in primary head kidney and THK cells from different temperature extraction methods, low temperature extracted ABGME, SSE or mixed compound (ABGME + SSE, A + S) gave better immune stimulating effect. In the gene expression assay, a kind of synergistic effect was seen in A+S group, which also significantly induces the phagocytic activity of liver and spleen macrophages. In the in vivo experiment, challenge test by intraperitoneal injection of Taiwan tilapia with Streptococcus agalactiae was performed after 1, 3, 7, 14 and 28 days continuous feeding of Taiwan tilapia with different percentage (1%, 2.5% and 5%) of growing substrate of A. blazei (ABGM) or S. suiae powder (SS). By comparing different formula of feed and feeding days, results showed that continuous feeding of Taiwan tilapia 7 days with 1% growing substrate of A. blazei (ABGM) or 1% S. suiae powder (SS) had better protection, and feeding of Taiwan tilapia 28 days with 5% growing substrate of A. blazei (ABGM) or 1% S. suiae powder (SS) had better protection.

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Development and application of agricultural by product as immunomodulation compound on Taiwan tilapia

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