A Microwell Array Device for Measuring Single-Cell Oxygen Consumption Rates Based on Phase-Based Lifetime Detection in Applications of Tgiv Infection and Anti-Viral Drugs Screening

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簡歷

基本資料

Project title

Code/計畫編號

MOST105-2221-E019-037

Translated Name/計畫中文名

利用細胞耗氧磷光相位感測裝置探討藻類萃取物於抗台灣石斑虹彩病毒感染之效能分析

Project Coordinator/計畫主持人

Shih-Hao Huang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Mechanical and Mechatronic Engineering

Website

https://www.grb.gov.tw/search/planDetail?id=11897210

Year

2016

Start date/計畫起

01-08-2016

Expected Completion/計畫迄

01-07-2017

Bugetid/研究經費

961千元

ResearchField/研究領域

機械工程

"近年來，有許多的海藻萃取物已經被證實具有抑制病毒感染的功能，然而，海藻萃取物應用於抑制台灣石斑虹彩病毒感染的相關研究仍相當缺乏，亦缺少一有效快速檢測平台應用於抗病毒藥物的開發與篩選上。因此，本計劃將建立一個細胞耗氧磷光相位感測裝置應用於藻類萃取物作用於抗台灣石斑虹彩病毒感染之效能分析，了解四種主要大型藻類：綠藻(石蓴)、紅藻(鹿角菜)、褐藻(馬尾藻)、藍綠藻(螺旋藻)的海藻萃取物於抑制台灣石斑虹彩病毒感染的抗病毒感染的機制、以及海藻萃取物於細胞感染病毒前或感染病毒後的預防與治療效果。結合細胞培養微陣列晶片、氣動密閉作動裝置、與磷光相位差光學檢測系統，具有微小化、陣列化和自動化之目的，達到即時感測的功能，此裝置能夠即時監視細胞感染後不同階段的耗氧率，而耗氧率可反映細胞代謝的活性以評估海藻萃取物抑制病毒的能力，進而篩選出何種海藻萃取物具有最佳抑制毒感染效果與最佳抑制病毒感染的劑量、評估不同海藻萃取物在何種感染階段下加入海藻萃取物具有最佳抗病毒效果(預防效果)與有效抑制病毒感染作用時間(治療效果)。藉由整合樹狀微流道濃度梯度裝置至細胞培養微陣列晶片中，並加入陣列化光纖式磷光相位差光學檢測系統，能夠對不同濃度海藻萃取物抑制台灣石斑虹彩病毒感染進行大量效能評估，大幅縮短實驗所需之時程與人為的操作，篩選出最佳的海藻萃取物抑制台灣石斑虹彩病毒感染的劑量。" "Recently, the algae extracts from red, brown or green alga, have been proven to have ability to prevent from virus infection. However, the algae extracts used in the inhibition of TGIV (Grouper iridovirus of Taiwan) in grouper infection have not been paid much attention. Also, there is not a useful platform, which can fast screen the potent anti-viral drugs of TGIV. In this project, we propose a microwell array device for measuring cellular oxygen consumption rates (OCR) based on phase-based lifetime detection in applications of TGIV infection and anti-viral drugs screening. Measuring cellular OCRs, which can reflect cellular metabolic activity, enabled us to monitor the process of viral infection in real time and to rapidly determine the antiviral activity of potential drugs/chemical compounds. The device consists of three main components including: (1) A microwell array for single cell culture. Cells were seeded onto the oxygen-sensitive dye inside a microwell. (2) Pneumatically-driven lid to hermetically seal the microwell. This component also contains microfluidic channels for automatic drug injection. (3) A optical measuring system for single-cell oxygen consumption rates based on phase-based lifetime detection. Through our proposed device, we hope to develop the effective and quick bioassay platforms for screening the anti-viral drugs of TGIV, and use the platforms help us to study the infection mechanism and athogenesis of TGIV."

Keyword(s)

海藻萃取物
台灣石斑虹彩病毒
細胞耗氧
磷光相位感測
extracts of the algae
Grouper iridovirus of Taiwan (TGIV)
cell oxygen consumption rate
phase-based lifetime detection
drug screening

DSpace CRIS

A Microwell Array Device for Measuring Single-Cell Oxygen Consumption Rates Based on Phase-Based Lifetime Detection in Applications of Tgiv Infection and Anti-Viral Drugs Screening

基本資料

Description