Development of Self-Powered Biosensors for Multiple Physiological Index of a Free-Swimming Fish and Used in Application of Internet of Things (Iot)

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Project title

Code/計畫編號

MOST108-2221-E019-033

Translated Name/計畫中文名

開發自體供電多項魚類生理指標感測裝置與物連網IOT之應用

Project Coordinator/計畫主持人

Shih-Hao Huang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Mechanical and Mechatronic Engineering

Website

https://www.grb.gov.tw/search/planDetail?id=13125975

Year

2019

Start date/計畫起

01-08-2019

Expected Completion/計畫迄

31-07-2020

Bugetid/研究經費

943千元

ResearchField/研究領域

能源工程

魚體內的血糖和乳酸濃度的變化是生理狀態異常的重要指標，血乳酸濃度是疲勞的指標、血糖高低與魚的營養狀態有相關。本研究完成兩個重要核心技術：(1)完成自體供電多項魚類生理指標感測裝置，製作探針式酵素燃料電池裝置並植入吳郭魚魚體內進行葡萄糖與乳酸的濃度量測、 (2)完成自行撰寫的手機App程式與動態影像分析程式拍攝與讀取LED燈閃爍頻率，得知吳郭魚魚體內葡萄糖與乳酸濃度。探針式酵素燃料電池裝置主要利用葡萄糖去氫酶(GDH)或乳酸去氫酶(LDH)作為陽極、膽紅素氧化酶(BOD)作為陰極的生物觸媒，建構出利用葡萄糖或乳酸和氧氣為反應物之酵素燃料電池，並外接升壓電荷幫浦電路進行充放電過程，分別驅動紅或綠光LED燈閃爍，閃爍的頻率正比於魚體內葡萄糖(紅光)、乳酸((綠光)的濃度，達到自體供電多項魚類生理指標感測，利用手機拍攝並藉由自行撰寫的手機App程式讀取2顆紅綠光LED燈閃爍頻率，可即時得知多項魚類生理指標、藉由藍牙傳輸至雲端記錄與分析。在血糖濃度10-180 mg/dL與乳酸濃度5-144 mg/dL的檢測範圍內，LED閃爍頻率與血糖 /乳酸濃度成線性比例。此外，藉由2顆紅綠光LED燈區分血糖與乳酸量測，成功完成同時量測不同血糖與乳酸比例的混合溶液與不同條件下魚血成分量測。再者，成功展示即時監測逐漸降溫冷休克(cold shock)處理下吳郭魚水下活體生理狀態量測的可行性：在水溫為 25℃的魚缸中的活體吳郭魚，其尾部插入自體供電生理指標感測裝置，產生的功率表現為開路電壓為0.41 V，在0.25 V時最大功率密度為6.3μW/cm2，電流密度為25μA/cm2。通過將水溫從25℃逐漸降低到15℃，在0.27 V時，發電量增加到最大功率密度 8.6μW/cm2，電流密度31μA/cm2。在25℃15℃時，通過閃爍頻率測定吳郭魚的血糖濃度分別為44 mg/dL和98 mg/dL。當水溫下降至 15℃，乳酸則是從原本的21 mg/dL些微上升再降到比原本還低的9 mg/dL，在緊迫狀況下，會造成壓力賀爾蒙，為了應付環境衝擊而生理調節所需能量之需求量增加所致，肝臟會消耗乳酸，轉換為血糖，導致血糖上升，乳酸下降。he changes of blood glucose and lactic acid concentration in fish are important indicators of abnormal physiological status. Blood lactic acid concentration is an indicator of fatigue. The level of blood sugar is related to the nutritional status of fish. We have successfully developed a self-powered glucose and lactate biosensor consisting of a needle-type enzymatic biofuel cell (N-EFC), which was operated underwater and connected to a charge pump integrated circuit (IC) and a light emitting diode (LED) as the indicator. The N-EFC consisted of a needle bioanode, which was inserted into the caudal area of a living fish (Tilapia) to access biofuels, and a gas-diffusion biocathode sealed in an airtight bag. The N-EFC was immersed entirely in the water and connected to a charge pump IC with a capacitor, which enabled charging and discharging of the bioelectricity generated from the N-EFC to blink an LED. Using a smartphone, the glucose or lactate concentration can be determined by observing the LED blinking frequencies that are linearly proportional to the blood glucose and lactate concentration within a detection range of 10–180 mg/dL and 5-144 mg/dL. We have successfully demonstrated the feasibility of the selfpowered glucose and lactate biosensor used to continuously monitor the physiological status of free-swimming fish treated with cold shock in real time. The power generated by a free-swimming fish with an N-EFC inserted into its caudal area, swimming in a fish tank with a water temperature (Tw) of 25 °C, exhibited an open circuit voltage of 0.41 V and a maximum power density of 6.3 μW/cm2 at 0.25 V with a current density of 25 μA/cm2. By gradually decreasing Tw from 25 °C to 15 °C, the power generation increased to a maximum power density of 8.6 μW/cm2 at 0.27 V with a current density of 31 μA/cm2. The blood glucose levels of the free-swimming fish at 25 °C and 15 °C determined by the blinking frequencies were 44 mg/dL and 98 mg/dL, respectively. By gradually decreasing Tw from 25 °C to 15 °C, the lactic acid rises slightly from the original 21 mg/dL and then drops to 9 mg/dL which is lower than the original.

Keyword(s)

酵素燃料電池
葡萄糖
膽固醇
乳酸
吳郭魚
Enzymatic biofuel cell
glucose
cholesterol
lactate acid
Tilapia

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Development of Self-Powered Biosensors for Multiple Physiological Index of a Free-Swimming Fish and Used in Application of Internet of Things (Iot)

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