The Functional Roles of Single-Minded Gene and Toxicities of Environmental Hormones in Zebrafish Head Skeleton Development (I)

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基本資料

Project title

Code/計畫編號

NSC91-2313-B019-033

Translated Name/計畫中文名

Single-Minded基因與環境荷爾蒙戴奧辛對斑馬魚頭骨發育的影響(I)

Project Coordinator/計畫主持人

Chin-Hwa Hu

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Bioscience and Biotechnology

Website

https://www.grb.gov.tw/search/planDetail?id=758539

Year

2002

Start date/計畫起

01-08-2002

Expected Completion/計畫迄

01-07-2003

Bugetid/研究經費

1350千元

ResearchField/研究領域

生物科學
生物技術(理)

在本期研究中，我們針對了Single-minded基因對斑馬魚頭骨發育的情形利用Morpholino的方式進行該基因的抑制後觀察到頭股的發育發生了相當嚴重的影響，目前正以各種標示基因觀察受Single-minded基因抑制影響的下游基因與頭骨發育間的關係。除此之外，對於環境荷爾蒙與頭骨發育間的關係，我們已完成詳細的觀察，並利用各種標示基因觀察受戴奧辛影響的情形。這項工作已完成論文撰寫的工作，目前已投稿於Molecular pharmacology接受審查中。在下一期的研究中，我們將進一步探討Single-minded基因與其他胚胎發育調控基因間的互動關係，以釐清該基因在胚胎發育中所扮演的角色。 To investigate the function of single-minded gene in vertebrate development, we have microinjected single-minded 2-specific morpholino into zebrafish embryo at 1-cell stage and observed the effects on head skeleton development. By using various markers and Alcian blue staining, we have observed severe cranial cartilage defects in sim2-morpholino injected embryos. In addition, the cranial cartilage development was severely disrupted by waterborne 2,3,7,8-tetrachlorodibenzo-p-dioxin. Specifically, the expression profiles of cartilage markers chm1, col2a1 and the col2a1 upstream regulator sox9a, were all disturbed, but not eliminated, by TCDD exposure. It suggested that the TCDD disrupted the process of precartilage condensation, but not chondrogenesis, during cartilage morphogenesis.

Keyword(s)

斑馬魚
頭骨
戴奧辛
發育
環境荷爾蒙
Zebrafish
Skull
Single-minded gene
Dioxin
Development
Environmental hormone

DSpace CRIS

The Functional Roles of Single-Minded Gene and Toxicities of Environmental Hormones in Zebrafish Head Skeleton Development (I)

基本資料

Description