The Functional Roles of Single-Minded Gene and Toxicities of Environmental Hormones in Zebrafish Head Skeleton Development (II)

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基本資料

Project title

Code/計畫編號

NSC92-2313-B019-031

Translated Name/計畫中文名

Single-Minded基因與環境荷爾蒙戴奧辛對斑馬魚頭骨發育的影響(II)

Project Coordinator/計畫主持人

Chin-Hwa Hu

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Bioscience and Biotechnology

Website

https://www.grb.gov.tw/search/planDetail?id=848933

Year

2003

Start date/計畫起

01-08-2003

Expected Completion/計畫迄

01-07-2004

Bugetid/研究經費

1242千元

ResearchField/研究領域

生物技術(理)
生物科學

本實驗室在先前的研究中發現斑馬魚的sim2基因在發育初期即在間腦，腦室，鰓弧以及腸胃等處有相當專一性的表現。在本計畫的第一期實驗中，即探討了sim2在斑馬魚頭骨發育的過程中所扮演的角色。在本期的計畫實驗中，我們主要在探討環境荷爾蒙戴奧辛對於頭骨發育的影響，發現了戴奧辛會造成斑馬魚頭骨機型發育的情況，這項研究結果稍早由Teraoka等人發表（Toxicol Sci. 65,192-199）。但是本實驗室在研究過程中發現色素抑制劑phenylthiourea (PTU)會與戴奧辛產生擷抗作用，明顯降低戴奧辛所誘發的cyp1a1基因的活性。這項實驗推翻了一般對於PTU的看法，認為PTU對於斑馬魚胚胎不會產生影響的推論。在實驗中，我們發現PTU本身會誘發較低量的cyp1a1基因的表現，而這項基因的調控是受到AHR所控制。這項研究將於今年七月發表於Biochemical Pharmacology。本計畫第三期，我們將持續對sim2以及arnt2基因對於腦神經與頭骨的發育進行持續的研究，預期將會有所突破。During embryogenesis, sim2 mRNA appears in the animal hemisphere as early as 3 hpf and is expressed in a widespread pattern throughout the epiblast at the 75% epiboly stage, During the segmentation stae, sim2 mRNA is prominently expressed in the primordium of the hindbrain and appears as a transverse stripe in the epithelial layers of the mid-diencephalic boundary (MDB). In sim2-knockdown embryo, the craniofacial skeleton development was disrupted. To reveal the mechanism of TCDD-mediated defect in fish embryo, we have investigated the head skeleton development in TCDD-treated embryo by Alcian blue stain. It appeared that the development of cranial skeleton was disturbed. In addition, we found phenylthiourea (PTU) blocked TCDD-mediated cyp1a1 transcription via AHR signaling pathway. PTU induced a mild level of cyp1a1 transcription which was eliminated in AHR knockdown embryo. These results will be published (Biochemical Pharmacology 68 (2004) 63–71).

Keyword(s)

zebrafish
sim2
TCDD
phenylthiourea
cyp1a1
AHR

DSpace CRIS

The Functional Roles of Single-Minded Gene and Toxicities of Environmental Hormones in Zebrafish Head Skeleton Development (II)

基本資料

Description