Identification of Neuroendocrine and Steroidogenesis Systems of Sepioteuthis lessoniana by Functional Genomics and Metabolomics

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Project title

Code/計畫編號

NSC98-2313-B019-004-MY3

Translated Name/計畫中文名

萊氏擬烏賊神經內分泌與固醇荷爾蒙生合成系統的功能性基因體與代謝體學研究

Project Coordinator/計畫主持人

Wen-Shyong Tzou

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Bioscience and Biotechnology

Website

https://www.grb.gov.tw/search/planDetail?id=1996268

Year

2010

Start date/計畫起

01-08-2010

Expected Completion/計畫迄

31-07-2011

Bugetid/研究經費

1200千元

ResearchField/研究領域

生物技術(農)
漁業

"脊椎動物當中的生殖腺神經內分泌系統的調控是由是由一條源自於下視丘 (hypothalamus) 經由腦下垂體 (pituitary) 到生殖腺 (gonad) 的調控主軸所掌控，稱為hypothalamo-pituitary-gonadal axis(HPG axis)。促性腺釋放激素 (gonadotropin-releasing hormone, GnRH)則是位於整個調控主軸的起點，當GnRH 與腦下垂體細胞上的GnRH receptor 結合後，隨即促使腦下垂體細胞分泌黃體激素 (luteinizing hormone, LH) 及促濾泡成熟激素 (follicle-stimulating hormone, FSH)，進而促進性類固醇激素 (sex steroid hormone)的分泌。在許多分子演化與實驗的證據顯示，此調控主軸在脊椎動物的演化上具有相當高的保守性。本實驗目的在於建立海洋無脊椎動物軟體動物門頭足綱萊氏擬烏賊GnRH 調控系統的機制與在分子層級系統的保守情形。實驗方法首先利用EST 大量定序的方式及序列的保守性選殖萊氏擬烏賊神經組織、gonad 的GnRH 及GnRH 受體，並以rat 的腦下垂體及cos-7 cell 系統驗證GnRH 的生物活性，進而利用代謝體學的技術，追蹤不同季節的萊氏擬烏賊體內(神經組織、gonad 及血液)GnRH、固醇荷爾蒙的變化。我們也將進一步以重要的基因作為標誌，比較脊椎動物斑馬魚與軟體動物萊氏擬烏賊神經內分泌系統成體與胚胎的標誌基因表現的異同，以便勾勒出並比較神經內分泌系統的發育途徑。" "Hypothalamo-pituitary-gonadal axis (HPG axis) is a highly conserved neuroendocrine system across vertebrate. Gonadotropin-releasing hormone (GnRH) from hypothalamus stimulates the production of gonadotropin (luteinizing hormone, LH and follicle-stimulating hormone, FSH) in pituitary. LH and FSH then stimulates the release of sex steroid hormone (like estrogen) from gonad. This study is to use invertebrate Sepioteuthis lessoniana (a mollusk belonging to the cephalopod with highly evolved centralized neuron system) as a model system to investigate the neuroendocrine system focusing on GnRH and steroidogenesis. We will extract mRNA from brain and gonad of Sepioteuthis lessoniana and the following sequence determination and annotation will open the door to the gene repertoire of neuroendocrine system of this species. We will employ diverse biological assay systems, including rat pituitary cos-7 cell, to confirm the function of GnRH and GnRH receptor. We will also employ mass spectroscopy to analyze the seasonal change of GnRH and sex steroids in the blood and tissues of Sepioteuthis lessoniana. Finally, several genes involved in neuroendocrine system will be employed to undergo a comparative molecular anatomy study to delineate the developmental process of neuroendocrine system in Sepioteuthis lessoniana and zebrafish."

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Identification of Neuroendocrine and Steroidogenesis Systems of Sepioteuthis lessoniana by Functional Genomics and Metabolomics

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