Preparation and Application of Monoclonal Antibody toward Immunoactives Derived from Abalone

View Statistics Email Alert RSS Feed

Information

Details

Project title

Code/計畫編號

NSC85-2321-B019-016

Translated Name/計畫中文名

鮑魚免疫活化因子的單株抗體製作與應用

Project Coordinator/計畫主持人

Zwe-Ling Kong

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Food Science

Website

https://www.grb.gov.tw/search/planDetail?id=209383

Year

1996

Start date/計畫起

01-08-1995

Expected Completion/計畫迄

01-07-1996

Bugetid/研究經費

401千元

ResearchField/研究領域

食品科技(農)
生物技術(農)

在前期的研究中我們建立動物細胞實驗模式,用以研究篩選水產生理活性物質。據此已篩選出許多具高效免疫生理活性之水產抽出物,其中尤以鮑魚(Haliotis discus)之巨嗜細胞活化功能,細胞增生及促進抗體生成效果最為顯著。鮑魚熱水抽出成分經最終純化,所得的免疫活性本體為分子量約17Kds之耐熱性糖蛋白成分,其免疫生理活性包括:(1)白血球細胞凝集作用,(2)增加培養液中單位細胞的抗體生成量,(3)活化巨噬細胞,(4)提高細胞激素IL-1及TNF-a的分泌量)。本計畫乃主題為鮑魚耐熱性糖蛋白的單株抗體製作,在此研究中我們已成功地獲得並利用此單株抗體進行:(1)調查鮑魚組織中免疫生理活性糖蛋白的局在分布情形,(2)此免疫生理活性糖蛋白在水產食物中的含量分析,(3)製作親和力分離膠改善快速純化鮑魚耐熱性糖蛋白,(4)作為未來鮑魚耐熱性糖蛋白cDNA products的定量及標示物。 For screening and characterizing bioactives from marine resource, we have already developed the culture system and established all of the techniques. In our previous study, some shellfish extracts exhibited significant bioactivities in this model system. Furthermore, a heat-stable immunoactivator was purified from a salt aquerous extract of abalone (Haliotis discus). The extract of abalone (AHE) promoted cell proliferation, immunoglobulin secreting and macrophage-activating remarkly toward several cultured immune response cells, such as macrophages and B lymphocytes. We also prepared the monoclonal antibody of AHE principle this year, by methods of hybridoma system. A continue research related to abalone immunoactive, The ultimate achievement of us include: (1) realize the quantity and localization of the immunoactive within abalone tissue, (2) identify the other sea foods for comparison, (3) preparation of antibody-affinity column for fast purification of abalone immunoactive, (4) elucidation and confirmation of cDNA products for abalone immunoactive.

Keyword(s)

鮑魚
免疫活性物質
糖蛋白
單株抗體
Abalone
Immunoactives
Glycoprotein
Monoclonal antibody

DSpace CRIS

Preparation and Application of Monoclonal Antibody toward Immunoactives Derived from Abalone

Details

Description