Development of the Anti-Viral Brown Seaweed Sulfate Polysaccharide Extracts for Dengue Virus Type II (Dv2) Infection

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簡歷

基本資料

Project title

Code/計畫編號

MOST106-2622-B019-001-CC2

Translated Name/計畫中文名

開發抗第二型登革病毒感染之褐藻硫酸多醣萃取物

Project Coordinator/計畫主持人

Chang-Jer Wu

Funding Organization/主管機關

National Science and Technology Council

Co-Investigator(s)/共同執行人

詹伊琳

Department/Unit

Department of Food Science

Website

https://www.grb.gov.tw/search/planDetail?id=12485847

Year

2017

Start date/計畫起

01-02-2017

Expected Completion/計畫迄

01-01-2018

Bugetid/研究經費

1000千元

ResearchField/研究領域

漁業

"近年來，SARS、愛滋病、禽流感等各種病毒性傳染病陸續造成人類生命的恐慌後，如何開發有效地預防及治療病毒性傳染病的保健食品或藥物，已成了全世界人們心中的期待。登革病毒(dengue virus, DENV)為一個具有醣蛋白外套膜的單股正向RNA 病毒，台灣在過去曾發生三次大流行，顯示登革病毒感染在台灣為重要的流行性疾病。登革病毒主要藉由病媒蚊傳播感染至人體內，其感染後可能會引起登革熱 (dengue fever, DF)，或是更嚴重的疾病如：登革出血熱/登革休克症候群 (dengue hemorrhagic fever/ dengue shock syndrome, DHF/DSS)。然而免疫系統較差與第二次感染登革病毒的人其可能更容易造成登革出血熱。由於登革病毒所引起臨床症狀的機制尚不明確，也無有效的登革病毒疫苗來預防人類感染。然而近年來有越來越多的研究顯示出藻類硫酸多醣具有抗氧化、抗發炎、抗病毒、抗癌、抗凝血等作用，為具潛力之天然食物。於第一年核定的計畫中，我們已經從台灣海域的褐藻-半葉馬尾藻，以熱水萃取的方式製備出褐藻多醣萃取物，並且進行其多醣組成的分析，也利用利用病毒斑抑制率試驗評估褐藻多醣萃取物抑制登革病毒吸附感染的效果。實驗結果如下：1. 褐藻多醣萃取物中，半葉馬尾藻多醣硫酸根含量為29.2 ± 0.23%，而岩藻醣含量為79.96 g/g；2. 褐藻多醣萃取物在病毒斑形成抑制率試驗中，以病毒感染細胞前加入抑制效果最好。因此，第二年計畫將延續前述之結果，將利用本實驗室建立的登革病毒感染小鼠模式，來探討褐藻硫酸多醣萃取物中是否具有抑制第二型登革病毒感染及免疫調節的能力，並進一步去探討其可能的作用機制及餵食褐藻硫酸多醣萃取物是否可降低血球比容、減緩血小板低下與腸胃道出血情形，來提高感染小鼠之存活率。經由上述的研究，我們希望能發展出有效的抗登革病毒及免疫調節的褐藻硫酸多醣萃取物，以作為開發抗登革病毒及免疫調節的保健食品之應用。" "In this century, the health of human will be challenged by the newly emerged infectious disease, such as SARS, AIDS and Avian flu. Dengue virus (DENV) is a positive, single-stranded RNA virus with glycoprotein envelope, which caused three outbreaks in Taiwan. DENV infection has been shown an important epidemic disease in Taiwan. Dengue viral infections are one of the most important mosquito borne diseases in the world. They may give rise to dengue fever (DF), or a severe illness, dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). People with weakened immune systems as well as those with a second or subsequent dengue infection are believed to be at greater risk for developing dengue hemorrhagic fever. Mechanisms of how DENV causes clinical symptoms are not fully understood. There is still lacking of effective vaccines to prevent human from DENV infection. Hence, there is an urgent need for new treatments against viral infectious diseases. Recently, a number of investigators have found that the extracts of algae present a broad range of biological activities including antivirus, antitumor, immunomodulation and anticoagulant activity. In the ongoing project, we prepared polysaccharide extracts from a brown algae (Srgassum hemiphyllum) around Taiwan sea area by using hot-water extraction methods and analyze their components of polysaccharides. We also performed in vitro experiments to evaluate the inhibition effect of polysaccharide extracts in viral adsorption by using plaque reduction assay on BHK-21 cells. The result showed that sulfated polysaccharides of Srgassum hemiphyllum were 29.2 ± 0.23%, and the fucose content of Srgassum hemiphyllum were 79.96 g/g. Data also showed that brown algae extracts has the most viral inhibitory effect when added before viral adsorption in the plaque reduction assay. The next aim of this proposal will use our laboratory previously established the dengue virus infected mice model to find out the anti-DENV and immuno-modulatory activity of brown seaweed sulfate polysaccharide extracts and investigate the possible mechanism. We study whether the brown seaweed sulfate polysaccharide extracts can attenuates hematocrit, thrombocytopenia and gastrointestinal bleeding, and improves survival rate in neonatal mice after dengue virus infection. Through all these efforts, we hope to develop that the brown seaweed sulfate polysaccharide extracts may be used as a health food supplement of anti-DENV and immuno-modulatory activity agents."

Keyword(s)

登革病毒
登革熱
登革出血熱
登革休克症候群
褐藻硫酸多醣萃取物
抗病毒物質
免疫調節
保健食品
Dengue virus
Dengue fever
Dengue hemorrhagic fever
Dengue shock syndrome
Brown seaweed sulfate polysaccharide extracts
Anti-viral agent
Immuno-modulatory
Health food

DSpace CRIS

Development of the Anti-Viral Brown Seaweed Sulfate Polysaccharide Extracts for Dengue Virus Type II (Dv2) Infection

基本資料

Description