Development and Application of the Sulfate Polysaccharide Extracts from Cladosiphon Okamuranus as the Atopic Dermatitis Health Care Agent(I)

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基本資料

Project title

Code/計畫編號

MOST108-2622-B019-003-CC2

Translated Name/計畫中文名

水雲硫酸多醣萃取物做為改善異位性皮膚炎保健劑之開發與應用(1/2)

Project Coordinator/計畫主持人

Chang-Jer Wu

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Food Science

Website

https://www.grb.gov.tw/search/planDetail?id=13123857

Year

2019

Start date/計畫起

01-06-2019

Expected Completion/計畫迄

31-05-2020

Bugetid/研究經費

1200千元

ResearchField/研究領域

食品科技(工)

異位性皮膚炎是一種好發於孩童的慢性發炎性皮膚病，臨床症狀可見皮膚癢和皮膚乾燥。全球大約有5-20 %的孩童受此困擾。目前對異位性皮膚炎的治療主要以類固醇為主，雖然可有效控制皮膚炎病情，但長期使用會有造成皮膚萎縮、血管擴張及影響腎上腺素分泌而影響生長等副作用，造成很多病人及家屬會尋求健康食品等另類替代治療。近年來的研究文獻指出海藻硫酸多醣具有抗病毒、抗發炎、抗腫瘤、抗血栓、提高免疫力等保健功效。本篇論文主要利用細胞實驗及小鼠動物模式探討水雲 (Cladosiphon okamuranus) 多醣萃取物對於改善異位性皮膚炎及免疫調節的效果。P815肥大細胞脫顆粒測定與RAW264.7巨噬細胞一氧化氮抑制試驗中，說明水雲多醣萃取物具有抑制脫顆粒與抗發炎之作用。此外，本研究進一步在 DNCB誘導異位性皮膚炎的BALB/c小鼠模式中，探討口服水雲多醣與塗抹水雲多醣萃取物乳膏改善異位性皮膚炎及免疫調節的效果及其作用機制。實驗結果顯示，水雲多醣萃取物可以改善淋巴結發炎情況和表皮膚過度增生、減少嗜酸性顆粒球浸潤、降低異位性皮膚炎相關細胞因子。同時，本研究也揭露類固醇藥物所帶來的副作用。經由上述的研究，我們希望能發展出有效的水雲多醣萃取物產品類型，以作為改善異位性皮膚炎及免疫調節保健食品之應用。Atopic dermatitis (AD) is a chronic inflammatory skin disorder that occurs most frequently in children. AD characterized by itchy and dry skin and it affects approximately 5 to 20% of children in the world. Currently, the treatment that is most widely used to control AD is corticosteroids. Corticosteroids is effective in treating AD, however, there are concerns about the side effects such as skin atrophy, vasodilation, and inhibits the growth of children by affecting their adrenal gland function. Therefore, many patients and families seek complementary and alternative treatments such as health food. Recently, the sulfated polysaccharide extracts of seaweed have shown broad biological activities, including antiviral, antitumor, immunomodulatory and anticoagulant activity. This study investigated the sulfated polysaccharide extracts of Cladosiphon okamuranus for improve the atopic dermatitis and immunomodulation on in vitro and in vivo model. The result showed that the sulfated polysaccharide extracts of Cladosiphon okamuranus had the bioactivity of anti-degranulation and anti-inflammatory in P815 cell degranulation assay and RAW 264.7 cell NO-inhibition assay. Moreover, we investigated the improvement effects and immunomodulation mechanisms of atopic dermatitis treated orally and topically with the sulfated polysaccharide extracts of Cladosiphon okamuranus on the DNCB induced BALB/c mice model. The result indicated that improved the inflammatory of lymph nodes, decreased the epidermal hyperplasia, reduced the infiltration of eosinophils, and decreased the expressed of atopic dermatitis-associated cytokines. Simultaneously, we revealed corticosteroids had the side effects in vivo experiment. Through all these efforts, we hope to develop that sulfated polysaccharide extracts of Cladosiphon okamuranus may be used as the health food supplement to improve the atopic dermatitis and immunomodulation

Keyword(s)

異位性皮膚炎
健康食品
海藻
硫酸多醣
水雲
免疫調節
Atopic dermatitis
Health food
Seaweed
Sulfate polysaccharide
Cladosiphon okamuranus
Immunomodulation

DSpace CRIS

Development and Application of the Sulfate Polysaccharide Extracts from Cladosiphon Okamuranus as the Atopic Dermatitis Health Care Agent(I)

基本資料

Description