Establishment of the Genetic Information of Giant Grouper Brood Stock and Development of Functional Marker-Assisted Selection and Breeding for Application Integration( I ) -1

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基本資料

Project title

Code/計畫編號

MOST105-2321-B019-002

Translated Name/計畫中文名

建立石斑魚種原遺傳資訊及功能性標記開發輔助選育整合應用( I ) -1

Project Coordinator/計畫主持人

Hong-Yi Gong

Funding Organization/主管機關

National Science and Technology Council

Co-Investigator(s)/共同執行人

黃章文

Department/Unit

Department of Aquaculture

Website

https://www.grb.gov.tw/search/planDetail?id=11896729

Year

2016

Start date/計畫起

01-08-2016

Expected Completion/計畫迄

31-07-2017

Co-Investigator(s)

Chang-Wen Huang

Bugetid/研究經費

1800千元

ResearchField/研究領域

生物技術(農)

優質石斑魚選拔育種改良計晝為台灣現今刻不容緩的首要任務，以分子標記輔助種原庫選育之遺傳管理策略，為提升石斑魚品種改良之重要關鍵。本計晝合作廠商一龍佃海洋生物科技股份有限公司，在水產領域的生產端掌握各類石斑魚的繁養殖技術而聞名全球，多年來在種苗培育之遺傳改良過程面臨優質品系選種與開發效率為極待克服之問題。為能實質解決產業之瓶頸，本計晝共分成三年度執行，包括：（1)建立龍膽石斑與老虎斑種原庫之系譜追踨系統以及體表型與基因型之遺傳相關資訊；（2) 整合成長、抗病及繁殖等重要經濟性狀相關之功能性標記與基因型資訊，開發可輔助優質石斑魚選育之微衛星或單一核苷酸多型性分子標記；（3)運用已開發核苷酸變異序列之功能性分子標記，輔助石斑魚家系選拔及驗證，建立分子標記輔助石斑魚選拔育種之應用平台。透過本計晝所開發之分子標記，除可深入探討石斑魚肌肉成長、免疫及性別轉變等遺傳影響之作用機制外，亦能開發與提供石斑魚種苗產業縮短石斑魚育種時程之整合式分子標記，達真正解決優質石斑魚品種篩選所需耗費之養殖空間與時間及遺傳穩定度等問題。研究成果兼具基礎研究與產業應用價值，未來可提供業者建構完整產銷履歷及品牌認證之科學化管理，為水產養殖產業注入新元素。 At present, selective breeding for the genetic improvement of quality-based grouper is an urgent task in Taiwan. Genetics and brood stock management strategies of molecular marker-assisted selection and breeding are important for promoting improved varieties of seeds of grouper species. This project is being conducted in cooperation with Long Diann Marine Bio Technology Co., Ltd., which is the largest producer and pioneer in the artificial propagation of many grouper species known in Taiwan and worldwide. However, it remains important to overcome the potential problems of quality-based variety selection and development efficiency during seedling cultivation for genetic improvement. To effectively resolve these problems, this research project will use radio frequency identification and molecular marker-assisted breeding techniques to develop a strain with high economic value and great potential for developing quality-based grouper species in the international market. This project, which is expected to span over 3 years, will include the following objectives:⑴ establishment of a pedigree trace system and a relationship between the phenotype (identifiable traits) and genotype (heritable information) of giant and tiger grouper brood stocks; (2) determination of the economic importance of growth-, disease resistance-, and reproduction trait-related functional gene markers and genotype information and development of microsatellite or single nucleotide polymorphism molecular markers for assisted quality-based grouper selection and breeding; and (3) screening and development of functional molecular markers with functional nucleotide sequence variations to facilitate the family selection of groupers and determination of their effects on important economic traits in family breeding experiments. Our findings will help determine the genetic mechanisms underlying muscle growth, immunity, and gender transformation in groupers and will shorten the breeding platform for integrated molecular marker-assisted selection for the industrial applications of quality-based groupers, which requires large space, long time, and genetic stability. In addition, this study will assess the results of basic research for their value in industrial applications. The results of this study will help the grouper industry to scientifically manage complete traceability and branding certification and to create a new approach for increasing productivity and providing a competitive advantage in the future.

Keyword(s)

石斑魚
種原庫
遺傳資訊
功能性標記
標記輔助選育
Grouper
Brood Stock
Genetic Information
Functional Marker
Markers-Assisted Selection and Breeding

DSpace CRIS

Establishment of the Genetic Information of Giant Grouper Brood Stock and Development of Functional Marker-Assisted Selection and Breeding for Application Integration( I ) -1

基本資料

Description