研究成果

第 1 到 76 筆結果，共 76 筆。

公開日期標題作者來源出版物WOS全文
12024/6/1Cellular biocompatibility of cyanophycin substratum prepared with recombinant<i> Escherichia</i><i> coli' </i>(vol 105, pg 97, 2016)Tseng, Wen-Chi; Fang, Tsuei-Yun ; Chen, Sheng-YangBIOCHEMICAL ENGINEERING JOURNAL
22022Altering the substrate specificity of recombinant L-rhamnose isomerase from Thermoanaerobacterium saccharolyticum NTOU1 to favor D-allose productionWen-Chi Tseng; Yu-Chun Chen; Hao-Chin Chang; Chia-Jui Lin; Tsuei-Yun Fang Journal of Biotechnology1
32021Nanoassembly of UCST polypeptide for NIR-modulated drug releaseLin, Yu-Chih; Tsuei-Yun Fang ; Kao, Hsuan-Yu; Tseng, Wen-ChiBIOCHEMICAL ENGINEERING JOURNAL2
42021Fusion of the peptide derived from the acidic tail of alpha-synuclein improves the thermostability and soluble expression of recombinant Agrobacterium sp. D-allulose 3-epimeraseTseng, Wen-Chi; Hsu, Chung-Ting; Chang, Hao-Chin; Wang, Ming-Jun; Fang, Tsuei-Yun BIOCHEMICAL ENGINEERING JOURNAL4
52020Wound Healing Attributes of Polyelectrolyte Multilayers Prepared with Multi-l-arginyl-poly-l-aspartate Pairing with Hyaluronic Acid and gamma-Polyglutamic AcidUddin, Zeeshan; Fang, Tsuei-Yun ; Siao, Jyun-Yin; Tseng, Wen-ChiMacromolecular Bioscience
62019Upper Critical Solution Temperature-Type Thermal Response of Soluble Multi-l-Arginyl-Poly-l-Aspartic Acid (cyanophycin) Conjugated with MaltodextrinTseng, W. C.; Tsuei-Yun Fang ; Chen, C. Y.; Hsieh, Y. C.; Lai, W. L.Journal of Polymer Science Part a-Polymer Chemistry
72018Reversible Self-Assembly Nano vesicle of UCST Response Prepared with Multi-L-arginyl-poly-L-aspartate Conjugated with Polyethylene GlycolTseng, Wen-Chi; Tsuei-Yun Fang ; Lin, Yu-Chih; Huang, Shing-Jong; Huang, Yi-HaoBiomacromolecules
82018Characterization of a recombinant D-allulose 3-epimerase from Agrobacterium sp ATCC 31749 and identification of an important interfacial residueTseng, Wen-Chi; Chen, Chao-Nan; Hsu, Chung-Ting; Lee, Hsu-Chieh; Fang, Hong-Yi; Wang, Ming-Jun; Wu, Yi-Hung; Fang, Tsuei-Yun International Journal of Biological Macromolecules
92017Overexpression and characterization of a recombinant L-ribose isomerase from Actinotalea fermentans ATCC 43279Tseng, W. C.; Wu, T. J.; Chang, Y. J.; Cheng, H. W.; Tsuei-Yun Fang Journal of Biotechnology
102017Solubility and thermal response of fractionated cyanophycin prepared with recombinant Escherichia coliTseng, W. C.; Tsuei-Yun Fang ; Hsieh, Y. C.; Chen, C. Y.; Li, M. C.Journal of Biotechnology
112016Cellular biocompatibility of cyanophycin substratum prepared with recombinant Escherichia coliTseng, W. C.; Tsuei-Yun Fang ; Chen, S. Y.Biochemical Engineering Journal
122016以蛋白質工程及固定化提昇Agrobacterium sp. ATCC 31750來源重組 D-阿洛酮糖表異構酶之熱穩定性方弘懿; 李旭傑; 方翠筠 
132016以蛋白質工程提昇 Geodermatophilus obscurus DSM 43160 重組 L-核糖異構酶的酵素活性及熱穩定性張雅如; 吳泰徵; 方翠筠 
142015Cloning, expression, and characterization of Pseudomonas vesicularis MA103 beta-1,3-xylanase in Escherichia coli ClearColi BL21(DE3)Liang, Wen-Sing; Fang, Tsuei-Yun ; Lin, Hong-Ting ; Liu, Tristan C; Lu, Wen-Jung; Tzou, Wen-Shyong ; Tang, Shye-Jye ; Lin, Fu-Pang ; Liu, Shiu-Mei; Pan, Chorng-Liang Fisheries Science
152015CHARACTERIZATION OF A RECOMBINANT L-RIBOSE ISOMERASE FROM GEODERMATOPHILUS OBSCURUS DSM 43160 AND APPLICATION OF THIS ENZYME TO THE PRODUCTION OF L-RIBOSE FROM L-ARABINOSEHung, X. G.; Yu, M. Y.; Chen, Y. C.; Tsuei-Yun Fang Journal of Marine Science and Technology-Taiwan
162015利用海藻酸鹽固定具有D-阿洛酮糖表異構酶之大腸桿菌以生產D-阿洛酮糖方翠筠 ; 吳易皇; 方弘懿
172015利用海藻酸鹽固定具有L-鼠李糖異構酶之大腸桿菌以生產D-阿洛糖方翠筠 ; 李于昇; 張哲源
182014Characterization of a thermophilic l-arabinose isomerase from Thermoanaerobacterium saccharolyticum NTOU1Xing-Guang Hung; Wen-Chi Tseng; Shiu-Mei Liu; Wen-Shyong Tzou ; Tsuei-Yun Fang Biochemical Engineering Journal0
192014Identification of substrate-binding and selectivity-related residues of maltooligosyltrehalose synthase from the thermophilic archaeon Sulfolobus solfataricus ATCC 35092Tseng, W. C.; Lin, C. R.; Hung, X. G.; Wei, T. Y.; Chen, Y. C.; Fang T-Y. Enzyme and Microbial Technology
202013Expression of Synechocystis sp PCC6803 cyanophycin synthetase in Lactococcus lactis nisin-controlled gene expression system (NICE) and cyanophycin productionTseng, W. C.; Tsuei-Yun Fang ; Chang, K. C.; Chorng-Liang Pan Biochemical Engineering Journal
212013Interaction between Trehalose and MTHase from Sulfolobus solfataricus Studied by Theoretical Computation and Site-Directed MutagenesisFu, C. W.; Wang, Y. P.; Tsuei-Yun Fang ; Lin, T. H.Plos One
222013Mutation to alter the substrate specificity of a thermophilic L-rhamnose isomerase from Thermoanaerobacterium saccharolyticum NTOU1Fang, T. -Y. ; Lin, C. J.
232013pH responsive PEGylation through metal affinity for gene delivery mediated by histidine-grafted polyethylenimineTseng, W. C.; Su, L. Y.; Tsuei-Yun Fang Journal of Biomedical Materials Research Part B-Applied Biomaterials
242012Assessments of growth conditions on the production of cyanophycin by recombinant Escherichia coli strains expressing cyanophycin synthetase geneTseng, W. C.; Tsuei-Yun Fang ; Cho, C. Y.; Chen, P. S.; Tsai, C. S.Biotechnology Progress
252012Identification of Substrate-Binding and Selectivity-Related Residues of Maltooligosyltrehalose Synthase from the Thermophilic Archaeon Sulfolobus solfataricus ATCC 35092Tseng W-C; Lin C-R; Wei T-Y; Hung X-G; Fang T-Y 
262012Characterization of a Thermophilic L-Arabinose Isomerase from the Thermoanaerobacterium saccharolyticum NTOU1Hung X-G; Tseng W-C; Liu S-M; Tzou W-S; Fang T-Y 
272012Characterization of Glycogen phosphorylase from a thermophile Thermoanaerobacterium saccharolyticum NTOU1Fang T-Y ; Leong M.-T; Cheong W-S
282011Characterization of a Thermophilic L-Rhamnose Isomerase from Caldicellulosiruptor saccharolyticus ATCC 43494Lin, C. J.; Tseng, W. C.; Tsuei-Yun Fang Journal of Agricultural and Food Chemistry
292011Characterization of a salt-tolerant xylanase from Thermoanaerobacterium saccharolyticum NTOU1Hung, K. S.; Liu, S. M.; Tzou, W. S.; Fu-Pang Lin ; Sun, K. H.; Shye-Jye Tang ; Tsuei-Yun Fang Biotechnology Letters
302011Characterization of a novel GH10 thermostable, halophilic xylanase from the marine bacterium Thermoanaerobacterium saccharolyticum NTOU1Hung, K. S.; Liu, S. M.; Tzou, W. S.; Fu-Pang Lin ; Sun, K. H.; Shye-Jye Tang ; Tsuei-Yun Fang ; Chorng-Liang Pan Process Biochemistry
312010Characterization of a Thermophilic L-Rhamnose Isomerase from Thermoanaerobacterium saccharolyticum NTOU1Lin, C. J.; Tseng, W. C.; Lin, T. H.; Liu, S. M.; Tzu, W. S.; Tsuei-Yun Fang Journal of Agricultural and Food Chemistry
322010Simultaneous mutations up to six distal sites using a phosphorylation-free and ligase-free polymerase chain reaction-based mutagenesisTseng, Wen-Chi; Lin, Jing-Wei; Hung, Xing-Gung; Fang, Tsuei-Yun Analytical Biochemistry
332010The effect of pendant hydrophobicity on the biological efficacy of polyethylenimine conjugateSu, L. Y.; Tsuei-Yun Fang ; Tseng, W. C.Biochemical Engineering Journal
342010Effect of the Addition of Hydrogen Bond between the Substrate and Substrate-binding Site Residues on the Transglycosyl and Hydrolytic Activity of Maltooligosyltrehalose Synthase from Sulfolobus solfataricus ATCC 35092Fang T-Y. ; Wei T-Y.
352010Cloning, Expression and Characterization of L-Rhamnose Isomerase from Thermoanaerobacterium spLin J-W; Fang T-Y 
362010Characterization of L-Arabinose Isomerase from Thermoanaerobacterium sp.Hung X-G; Fang T-Y 
372010Caldicellulosiruptor saccharolyticus 鼠李糖異構酶基因選殖及特性探討林嘉銳; 林殿翔; 方翠筠 
382009Effects of Enhancers and Coating Substrates on the Transgene Expression Mediated by Branched PolyethylenimineTseng, W. C.; Tsuei-Yun Fang ; Chang, K. W.; Tang, C. H.; Su, L. Y.Journal of Applied Polymer Science
392009An efficient phosphorylation-free and ligase-free PCR-based method for multiple site-directed mutagenesis of up to six distal-sites simultaneouslyFang, T. Y. ; Lin, J. W.; Hung, X. G.
402009Thermoanaerobacterium sp. NTOU2 鼠李糖異構酶基因選殖及特性探討林嘉銳; 林殿翔; 方翠筠 
412008Identification of the essential catalytic residues and selectivity-related residues of maltooligosyltrehalose trehalohydrolase from the thermophilic archaeon Sulfolobus solfataricus ATCC 35092Fang T-Y. ; Tseng, W. C.; Shih, T. Y.; Wang, M. Y.Journal of Agricultural and Food Chemistry8
422008A novel megaprimed and ligase-free PCR-based site-directed mutagenesis methodTseng, Wen-Chi; Lin, Jing-Wei; Wei, Tsen-Yun; Fang, Tsuei-Yun Analytical biochemistry
432008Identification of the essential catalytic residues and selectivity-related residues ofmaltooligosyltrehalose trehalohydrolase from the thermophilic Archaeon Sulfolobus solfataricus ATCC 35092Fang T-Y ; Shih T-Y; Wang M-Y
442008A novel ligase-free and PCR-based site-directed mutagenesis method. The International Conference of Advenced Research on Marine BioresourcesFang T-Y ; Tseng W-C; Lin J-W; Wei T-Y
452008A. salmonicida MAEF108 Agarase AS-IIIb 與轉殖至 E.coli BL21 重組 Agarase AS-IIIb 之生化性質及水解產物分析廖友豪; 許志維; 潘彥齊; 方翠筠 ; 鄒文雄; 潘崇良
462008A. salmonicida MAEF108 Agarase AS-IIIb 之基因以 RF-cloning 與 PCR 轉殖至酵母菌 Pichia 中之表現許志維; 廖友豪; 方翠筠 ; 鄒文雄; 林乃頌; 潘崇良
472008轉殖至 E.coli 之 A. salmonicida MAEF108 Agarase rAS-IIIb 誘導表現與純化之探討許志維; 廖友豪; 方翠筠 ; 鄒文雄; 潘崇良
482008龜山島海底熱泉嗜高溫菌所產生阿拉伯糖異構酶的基因選殖與特性探討黃幸光; 方翠筠 
492007Trehalose enhances transgene expression mediated by DNA-PEI complexesTseng, W. C.; Tang, C. H.; Tsuei-Yun Fang ; Su, L. Y.Biotechnology Progress
502007Using disaccharides to enhance in vitro and in vivo transgene expression mediated by a lipid-based gene delivery systemTseng, W. C.; Tang, C. H.; Tsuei-Yun Fang ; Su, L. Y.Journal of Gene Medicine
512007Protein engineering of Sulfolobus solfataricus maltooligosyltrehalose synthase to alter its selectivityTsuei-Yun Fang ; Tseng, W. C.; Pan, C. H.; Chun, Y. T.; Wang, M. Y.Journal of Agricultural and Food Chemistry
522007AGFD 104-Protein engineering of Sulfolobus solfataricus maltooligosyltrehalose synthase to alter its selectivityFang, T. Y ; Tseng, W. C; Pan, C. H; Chung, Y. T
532007Assessment of hydrogen bond between maltooligosyltrehalose synthase and maltopentose by computer simulation and site-directed mutagenesisTseng W-C; Fang T-Y ; Lin C-R
542007Protein engineering of Sulfolobus solfataricus maltooligosyltrehalose synthase to alter its selectivityFang T-Y ; Tseng W-C; Pan C-H; Chung Y-T
552007以電腦模擬及定位突變評估麥芽糖苷海藻糖生成酶與麥芽寡糖間之氫鍵林嘉銳; 曾文祺; 方翠筠 
562007麥芽寡糖苷海藻糖水解酶的基因轉殖與特性探討黃幸光; 蘇惠君; 方翠筠 
572006Expression, purification, and characterization of the maltooligosyltrehalose trehalohydrolase from the thermophilic Archaeon Sulfolobus solfataricus ATCC 35092Tsuei-Yun Fang ; Tseng, W. C.; Guo, M. S.; Shih, T. Y.; Hung, X. G.Journal of Agricultural and Food Chemistry
582006Mutations on aromatic residues of the active site to alter selectivity of the Sulfolobus solfataricus maltooligosyltrehalose synthaseTsuei-Yun Fang ; Tseng, W. C.; Chung, Y. T.; Pan, C. H.Journal of Agricultural and Food Chemistry15
592005Characterization of the thermophilic isoamylase from the thermophilic archaeon Sulfolobus solfataricus ATCC 35092Tsuei-Yun Fang ; Tseng, W. C.; Yu, C. J.; Shih, T. Y.Journal of Molecular Catalysis B-Enzymatic20
602005Dependence of transgene expression and the relative buffering capacity of dextran-grafted polyethylenimineTseng, Wen-Chi; Fang, Tsuei-Yun ; Su, Ling-Yu; Tang, Chien-HsiangMolecular Pharmaceutics41
612004The role of dextran conjugation in transfection mediated by dextran-grafted polyethylenimineTseng, Wen-Chi; Tang, Chien-Hsiang; Fang, Tsuei-Yun The journal of gene medicine
622004Characterization of the trehalosyl dextrin-forming enzyme from the thermophilic archaeon Sulfolobus solfataricus ATCC 35092Fang, TY ; Hung, XG; Shih, TY; Tseng, WCExtremophiles25
632004Effects of buffer ions on isopropanol-enhanced plasmid purification using Q-SepharoseTseng, Wen‐Chi; Jian, Li‐Min; Fang, Tsuei‐Yun Separation Science and Technology
642004Effect of alcohol on purification of plasmid DNA using ion-exchange membraneTseng, Wen-Chi; Ho, Fu-Lyn; Fang, Tsuei-Yun ; Suen, Shing-YiJournal of Membrane Science
652004Characterization of the trehalosyl dextrin forming enzyme from the thermophilic Archaeon Sulfolobus solfataricus ATCC 35092Fang T-Y ; Hung X-G; Shih T-Y; Tseng W-C
662003Starch-binding domain shuffling in Aspergillus niger glucoamylaseCornett, Catherine A G; Fang, Tsuei-Yun ; Reilly, Peter J; Ford, ClarkProtein engineering
672000An additional H-bond in the alpha 1 beta 2 interface as the structural basis for the low oxygen affinity and high cooperativity of a novel recombinant hemoglobin (beta L105W)Fang, T Y ; Simplaceanu, V; Tsai, C H; Ho, N T; Ho, CBiochemistry
682000Novel recombinant hemoglobin, rHb (beta N108Q), with low oxygen affinity, high cooperativity, and stability against autoxidationTsai, CH; Fang, TY ; Ho, CBIOCHEMISTRY
692000Chain-selective isotopic labeling for NMR studies of large multimeric proteins: application to hemoglobinSimplaceanu, V; Lukin, J A; Fang, T Y ; Zou, M; Ho, N T; Ho, CBiophysical journal
701999Assessment of roles of surface histidyl residues in the molecular basis of the Bohr effect and of beta 143 histidine in the binding of 2,3-bisphosphoglycerate in human normal adult hemoglobinFang, TY ; Zou, M; Simplaceanu, V; Ho, NT; Ho, CBIOCHEMISTRY
711998Identification of a phosphate regulatory site and a low affinity binding site for glucose 6-phosphate in the N-terminal half of human brain hexokinaseFang, T Y ; Alechina, O; Aleshin, A E; Fromm, H J; Honzatko, R BThe Journal of biological chemistry
721998Protein engineering of Aspergillus awamori glucoamylase to increase its pH optimumFang, TY ; Ford, CPROTEIN ENGINEERING
731998Mutations to alter Aspergillus awamori glucoamylase selectivity.: I.: Tyr48Phe49→Trp, Tyr116→Trp, Tyr175→Phe, Arg241→Lys, Ser411→Ala and Ser411→GlyFang, TY ; Coutinho, PM; Ford, CPROTEIN ENGINEERING
741998Mutations to alter Aspergillus awamori glucoamylase selectivity. II. Mutation of residues 119 and 121T Y Fang ; R B Honzatko; P J Reilly; C FordPROTEIN ENGINEERING
751994Improved elution of isoamylase adsorbed on raw starch and the preservation of purified enzymeLin, Long-Liu; Tsuei-Yun Fang ; Chu, Wen-Shen; Hsu, Wen-HweiLetters in Applied Microbiology
761994Recovery of isoamylase from Pseudomonas amyloderamosa by adsorption-elution on raw starchTsuei-Yun Fang ; Long-Liu Lin; Wen-Hwei HsuEnzyme and Microbial Technology