DSpace 集合:http://scholars.ntou.edu.tw/handle/123456789/197792024-03-25T17:48:50Z2024-03-25T17:48:50ZA fish herpesvirus highlights functional diversities among Z alpha domains related to phase separation induction and A-to-Z conversionDiallo, Mamadou AmadouPirotte, SebastienHu, YunlongMorvan, LeaRakus, KrzysztofSuarez, Nicolas M.Po-Tsang LeeSaneyoshi, HisaoXu, YanDavison, Andrew J.Tompa, PeterSussman, Joel L.Vanderplasschen, Alainhttp://scholars.ntou.edu.tw/handle/123456789/224632022-10-28T02:44:13Z2022-09-01T00:00:00Z標題: A fish herpesvirus highlights functional diversities among Z alpha domains related to phase separation induction and A-to-Z conversion
作者: Diallo, Mamadou Amadou; Pirotte, Sebastien; Hu, Yunlong; Morvan, Lea; Rakus, Krzysztof; Suarez, Nicolas M.; Po-Tsang Lee; Saneyoshi, Hisao; Xu, Yan; Davison, Andrew J.; Tompa, Peter; Sussman, Joel L.; Vanderplasschen, Alain
摘要: Zalpha (Z alpha) domains bind to left-handed Z-DNA and Z-RNA. The Z alpha domain protein family includes cellular (ADAR1, ZBP1 and PKZ) and viral (vaccinia virus E3 and cyprinid herpesvirus 3 (CyHV-3) ORF112) proteins. We studied CyHV-3 ORF112, which contains an intrinsically disordered region and a Z alpha domain. Genome editing of CyHV-3 indicated that the expression of only the Z alpha domain of ORF112 was sufficient for normal viral replication in cell culture and virulence in carp. In contrast, its deletion was lethal for the virus. These observations revealed the potential of the CyHV-3 model as a unique platform to compare the exchangeability of Z alpha domains expressed alone in living cells. Attempts to rescue the ORF112 deletion by a broad spectrum of cellular, viral, and artificial Z alpha domains showed that only those expressing Z-binding activity, the capacity to induce liquid-liquid phase separation (LLPS), and A-to-Z conversion, could rescue viral replication. For the first time, this study reports the ability of some Z alpha domains to induce LLPS and supports the biological relevance of dsRNA A-to-Z conversion mediated by Z alpha domains. This study expands the functional diversity of Z alpha domains and stimulates new hypotheses concerning the mechanisms of action of proteins containing Z alpha domains.2022-09-01T00:00:00ZGreen Synthesis of Endolichenic Fungi Functionalized Silver Nanoparticles: The Role in Antimicrobial, Anti-Cancer, and Mosquitocidal ActivitiesMohanta, Yugal KishoreNayak, DebasisMishra, Awdhesh KumarChakrabartty, IshaniRay, Manjit KumarMohanta, Tapan KumarTayung, KumanandaRajaganesh, RajapandianVasanthakumaran, MuruganMuthupandian, SaravananMurugan, KadarkaraiSharma, GouriduttaDahms, Hans-UweHwang, Jiang-Shiouhttp://scholars.ntou.edu.tw/handle/123456789/224622022-10-04T07:33:40Z2022-09-01T00:00:00Z標題: Green Synthesis of Endolichenic Fungi Functionalized Silver Nanoparticles: The Role in Antimicrobial, Anti-Cancer, and Mosquitocidal Activities
作者: Mohanta, Yugal Kishore; Nayak, Debasis; Mishra, Awdhesh Kumar; Chakrabartty, Ishani; Ray, Manjit Kumar; Mohanta, Tapan Kumar; Tayung, Kumananda; Rajaganesh, Rajapandian; Vasanthakumaran, Murugan; Muthupandian, Saravanan; Murugan, Kadarkarai; Sharma, Gouridutta; Dahms, Hans-Uwe; Hwang, Jiang-Shiou
摘要: Green nanotechnology is currently a very crucial and indispensable technology for handling diverse problems regarding the living planet. The concoction of reactive oxygen species (ROS) and biologically synthesized silver nanoparticles (AgNPs) has opened new insights in cancer therapy. The current investigation caters to the concept of the involvement of a novel eco-friendly avenue to produce AgNPs employing the wild endolichenic fungus Talaromyces funiculosus. The synthesized Talaromyces funiculosus-AgNPs were evaluated with the aid of UV visible spectroscopy, dynamic light scattering (DLS), Fourier infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The synthesized Talaromyces funiculosus-AgNPs (TF-AgNPs) exhibited hemo-compatibility as evidenced by a hemolytic assay. Further, they were evaluated for their efficacy against foodborne pathogens Staphylococcus aureus, Streptococcus faecalis, Listeria innocua, and Micrococcus luteus and nosocomial Pseudomonas aeruginosa, Escherichia coli, Vibrio cholerae, and Bacillus subtilis bacterial strains. The synthesized TF-AgNPs displayed cytotoxicity in a dose-dependent manner against MDA-MB-231 breast carcinoma cells and eventually condensed the chromatin material observed through the Hoechst 33342 stain. Subsequent analysis using flow cytometry and fluorescence microscopy provided the inference of a possible role of intracellular ROS (OH-, O-, H2O2, and O-2(-)) radicals in the destruction of mitochondria, DNA machinery, the nucleus, and overall damage of the cellular machinery of breast cancerous cells. The combined effect of predation by the cyclopoid copepod Mesocyclops aspericornis and TF-AgNPS for the larval management of dengue vectors were provided. A promising larval control was evident after the conjunction of both predatory organisms and bio-fabricated nanoparticles. Thus, this study provides a novel, cost-effective, extracellular approach of TF-AgNPs production with hemo-compatible, antioxidant, and antimicrobial efficacy against both human and foodborne pathogens with cytotoxicity (dose dependent) towards MDA-MB-231 breast carcinoma.2022-09-01T00:00:00ZFucoidan from Sargassum hemiphyllum inhibits the stemness of cancer stem cells and epithelial-mesenchymal transitions in bladder cancer cellsSung, Chun-JuWang, Hsiao-HsienSun, Kuang-HuiHsieh, Chii-ChengHuang, RogerSun, Guang-HuanTang, Shye-Jyehttp://scholars.ntou.edu.tw/handle/123456789/224612022-10-04T07:33:41Z2022-11-30T00:00:00Z標題: Fucoidan from Sargassum hemiphyllum inhibits the stemness of cancer stem cells and epithelial-mesenchymal transitions in bladder cancer cells
作者: Sung, Chun-Ju; Wang, Hsiao-Hsien; Sun, Kuang-Hui; Hsieh, Chii-Cheng; Huang, Roger; Sun, Guang-Huan; Tang, Shye-Jye
摘要: A variety of anticancer activities have been established for fucoidan from brown algae, whereas whether cancer stem cells (CSCs) are inhibited by sulfated polysaccharides is unexplored. In this study, fucoidan extracted from Sargassum hemiphyllum was showed heat stable and might tolerate 140 C treatment. Fucoidan did not exhibit cytotoxicity in 5637 and T24 bladder cancer cells. After fucoidan treatment, the stress fibers were aggregated into thick and abundant underneath the plasma membrane and getting around the cells, and the structure of F -actin showed a remarkable change in the filopodial protrusion in T24 and 5637 cells. Using culture inserts, transwell assays and time lapse recordings showed that fucoidan inhibited cell migration. In the epithelial-mesenchymal transition (EMT), fucoidan downregulated the expression of vimentin, a mesenchymal marker, and upregulated the expression of E-cadherin, an epithelial marker. Additionally, the transcription levels of Snail, Slug, Twist1, Twist2, MMP2 and MMP9 were significantly decreased by fucoidan, indicating EMT suppression. CSCs are implicated in tumor initiation, metastatic spread, drug resistance and tumor recurrence. Our results showed that fucoidan inhibited stemness gene expression and sphere formation in bladder CSCs. For the first time, our findings demonstrated that fucoidan inhibits CSC formation and provides evidence as potential anti-cancer therapy.2022-11-30T00:00:00ZOligo-Fucoidan supplementation enhances the effect of Olaparib on preventing metastasis and recurrence of triple-negative breast cancer in miceChen, Li-MeiYang, Pao-PaoAl Haq, Aushia TanzihHwang, Pai-AnLai, You-ChenWeng, Yueh-ShanChen, Michelle AudreyHsu, Hsin-Linghttp://scholars.ntou.edu.tw/handle/123456789/223392022-10-04T07:33:40Z2022-09-15T00:00:00Z標題: Oligo-Fucoidan supplementation enhances the effect of Olaparib on preventing metastasis and recurrence of triple-negative breast cancer in mice
作者: Chen, Li-Mei; Yang, Pao-Pao; Al Haq, Aushia Tanzih; Hwang, Pai-An; Lai, You-Chen; Weng, Yueh-Shan; Chen, Michelle Audrey; Hsu, Hsin-Ling
摘要: Background Seaweed polysaccharides have been recommended as anticancer supplements and for boosting human health; however, their benefits in the treatment of triple-negative breast cancers (TNBCs) and improving immune surveillance remain unclear. Olaparib is a first-in-class poly (ADP-ribose) polymerase inhibitor. Oligo-Fucoidan, a low-molecular-weight sulfated polysaccharide purified from brown seaweed (Laminaria japonica), exhibits significant bioactivities that may aid in disease management. Methods Macrophage polarity, clonogenic assays, cancer stemness properties, cancer cell trajectory, glucose metabolism, the TNBC 4T1 cells and a 4T1 syngeneic mouse model were used to inspect the therapeutic effects of olaparib and Oligo-Fucoidan supplementation on TNBC aggressiveness and microenvironment. Results Olaparib treatment increased sub-G1 cell death and G2/M arrest in TNBC cells, and these effects were enhanced when Oligo-Fucoidan was added to treat the TNBC cells. The levels of Rad51 and programmed death-ligand 1 (PD-L1) and the activation of epidermal growth factor receptor (EGFR) and adenosine 5 '-monophosphate (AMP)-activated protein kinase (AMPK) facilitate drug resistance and TNBC metastasis. However, the combination of olaparib and Oligo-Fucoidan synergistically reduced Rad51 and PD-L1 levels, as well as the activity of EGFR and AMPK; consistently, TNBC cytotoxicity and stemness were inhibited. Oligo-Fucoidan plus olaparib better inhibited the formation of TNBC stem cell mammospheroids with decreased subpopulations of CD44(high)/CD24(low) and EpCAM(high) cells than monotherapy. Importantly, Oligo-Fucoidan plus olaparib repressed the oncogenic interleukin-6 (IL-6)/p-EGFR/PD-L1 pathway, glucose uptake and lactate production. Oligo-Fucoidan induced immunoactive and antitumoral M1 macrophages and attenuated the side effects of olaparib, such as the promotion on immunosuppressive and protumoral M2 macrophages. Furthermore, olaparib plus Oligo-Fucoidan dramatically suppressed M2 macrophage invasiveness and repolarized M2 to the M0-like (F4/80(high)) and M1-like (CD80(high) and CD86(high)) phenotypes. In addition, olaparib- and Oligo-Fucoidan-pretreated TNBC cells resulted in the polarization of M0 macrophages into CD80(+) M1 but not CD163(+) M2 macrophages. Importantly, olaparib supplemented with oral administration of Oligo-Fucoidan in mice inhibited postsurgical TNBC recurrence and metastasis with increased cytotoxic T cells in the lymphatic system and decreased regulatory T cells and M2 macrophages in tumors. Conclusion Olaparib supplemented with natural compound Oligo-Fucoidan is a novel therapeutic strategy for reprogramming cancer stemness, metabolism and the microenvironment to prevent local postsurgical recurrence and distant metastasis. The combination therapy may advance therapeutic efficacy that prevent metastasis, chemoresistance and mortality in TNBC patients.2022-09-15T00:00:00Z