http://scholars.ntou.edu.tw/handle/123456789/4 2026-04-08T13:38:36Z 2026-04-08T13:38:36Z Ly, Tien H Huang, Cheng-Ting Lee, Meng-Chou Thanh, Vo V Diep, Doan X http://scholars.ntou.edu.tw/handle/123456789/26552 2026-03-16T09:11:06Z 2023-12-01T00:00:00Z

標題: Determination of the growth performance and reproductive season of wild Channa lucius (Cuvier, 1831) populations of genus Channa (Channidae, Perciformes) in the Mekong Delta, Vietnam 作者: Ly, Tien H; Huang, Cheng-Ting; Lee, Meng-Chou; Thanh, Vo V; Diep, Doan X 摘要: This study aimed to investigate the growth and spawning characteristics of wild Channa lucius (Cuvier, 1831) populations in the Mekong Delta, Vietnam. The study was carried out from March 2011 to February 2012. The length-weight relationship of C. lucius was determined according to the equations W = 0.0044L(3.2494), R-2 = 0.9157 (female fish) and W = 0.0047L(3.2178), R-2 = 0.9434 (male fish). The length and weight of the body for female fish ranged from 16.3 to 36.0 cm and 49.0 to 550.0 g, and for male fish, from 14.6 to 38.2 cm and 46.0 to 639.0 g, respectively. Female and male fish had almost similar lengths at first maturity (21.3958 cm for females and 21.3952 cm for males). The condition factor of female fish ranged from 0.842 to 0.864 and for male fish ranged from 0.848 to 0.874. The gonadosomatic index of female fish was highest in June (1.68%) and lowest in November (0.69%); that of male fish was highest in June (0.53%) and lowest in September (0.15%). The sexual maturity rate (SMR) of female fish peaked in May, with SMRs of 33.3% (stage III) and 58.3% (stage IV). In conclusion, C. lucius has a positive allometric range (b > 3), reaching sexual maturity when its length reaches 21.39 cm for both sexes, and the spawning season begins in December but peaks in May and June.

2023-12-01T00:00:00Z Windarto, Seto Hsu, Jue-Liang Lee, Meng-Chou http://scholars.ntou.edu.tw/handle/123456789/26551 2026-03-16T09:05:19Z 2024-06-01T00:00:00Z

標題: First report of antioxidant potential of peptide fraction derived from Colaconema formosanum (Rhodophyta) protein hydrolysates 作者: Windarto, Seto; Hsu, Jue-Liang; Lee, Meng-Chou 摘要: Foods that contain natural antioxidants can improve people's health and performance and make them more resistant to environmental stressors like diseases, lousy living conditions, and heat stress. Marine algae are well known for their function as an antioxidant. Colaconema formosanum is a new rhodophyte discovered in Taiwan and its bioactive activities are rarely studied. This work aims to prepare the protein hydrolysate fraction with antioxidant properties in C. formosanum using single enzyme or complex enzyme hydrolysis. Afterward, a 3 kDa molecular weight cut-off (MWCO) was used for centrifugation, and then fractionation was carried out using Strong Cation Exchange (SCX) Chromatography. The quantification of antioxidant activity was performed using the DPPH test. The results showed that the thermolysin hydrolysate (IC50 1.48 +/- 0.92 mg/mL) and the combination of trypsin-thermolysin hydrolysates (IC50 1.37 +/- 0.84 mg/mL) had the highest antioxidant activity. The fractionation procedure with strong cation exchange (SCX) yielded fraction C from thermolysin hydrolysates, which exhibited the most significant antioxidant activity with an IC50 value of 0.47 +/- 0.02 mg/mL. The fraction 4 derived from trypsin-thermolysin hydrolysates exhibited the most potent antioxidant activity (IC50: 0.34 +/- 0.002 mg/mL). No previous reports have been made about the antioxidant properties of the hydrolysate fractions derived from C. formosanum proteins. Consequently, these findings could serve as a foundation for exploring peptides that have potential therapeutic sources for reducing oxidative stress.

2024-06-01T00:00:00Z Windarto, Seto Lee, Meng-Chou Nursyam, Happy Hsu, Jue-Liang http://scholars.ntou.edu.tw/handle/123456789/26550 2026-03-16T08:58:14Z 2024-12-01T00:00:00Z

標題: A novel phycoerythrin-derived peptide from Colaconema formosanum: Synthesis, in vitro, and in silico study on angiotensin-converting enzyme (ACE) inhibitory activity 作者: Windarto, Seto; Lee, Meng-Chou; Nursyam, Happy; Hsu, Jue-Liang 摘要: Colaconema formosanum, the red algae used in this study, has never been documented for its bioactivities as an angiotensin-converting enzyme inhibitor (ACEI). This study aims to discover ACEI derived from C. formosanum with a mixture of enzymes and orthogonal bioassay-guided fractionation through in vitro and in silico approaches. LRDGEIILR (LR-9) was isolated from the protein hydrolysate of C. formosanum digested by a combination of proteases (pepsin and thermolysin). It is derived from phycoerythrin, a protein abundant in most red algae. The LR-9 has an IC50 value of 94.28 +/- 1.84 mu M. The kinetics of inhibition revealed LR-9 to be a competitive inhibitor. The results of kinetic studies were rationalized further using molecular docking, which showed that LR-9 interacts preferentially with critical residues in the ACE active site. Based on the toxicity prediction, LR-9 was non-toxic. This study suggests that ACEI peptides extracted from C. formosanum could be an alternative source for developing hypertension-treating functional foods.

2024-12-01T00:00:00Z Cheng, Wen-Ping Yeh, Han-Yang Chen, Yen-Ling Chen, Yi-Jung Sze, Fat-Tin Agassi Huang, Chi-Cheng Nan, Fan-Hua Fang, Ming-Chih Lee, Meng-Chou http://scholars.ntou.edu.tw/handle/123456789/26549 2026-03-16T09:18:25Z 0026-01-01T00:00:00Z

標題: Enhanced Production of Bioactive Polyunsaturated Fatty Acids and Pigments in Rhodosorus marinus: Optimization of Thermal and Photic Stress Conditions 作者: Cheng, Wen-Ping; Yeh, Han-Yang; Chen, Yen-Ling; Chen, Yi-Jung; Sze, Fat-Tin Agassi; Huang, Chi-Cheng; Nan, Fan-Hua; Fang, Ming-Chih; Lee, Meng-Chou 摘要: The marine unicellular red alga Rhodosorus marinus is a promising source of the valuable phycobiliprotein phycoerythrin and essential omega-3 polyunsaturated fatty acids (PUFAs), yet the environmental triggers for their optimal co-production remain to be fully elucidated. This study was conducted to investigate the effects of thermal and photic stress in terms of maximizing the yield of these high-value bioactive compounds. R. marinus was cultivated under a range of temperatures (18-24 degrees C) and light intensities (100-335 & micro;mol photons m-2 s-1) to assess its physiological and biochemical responses, particularly focusing on lipid accumulation. This study investigates the effects of thermal (18-24 degrees C) and photic (100-335 & micro;mol photons m-2 s-1) stress on the concurrent production of the valuable phycobiliprotein, phycoerythrin (PE), and essential omega-3 polyunsaturated fatty acids (PUFAs) in the marine red microalga Rhodosorus marinus. Fatty acid profiles were quantified using gas chromatography (GC), while pigment content was assessed via spectrophotometry. Statistical analyses, including one-way ANOVA and Tukey's post hoc test, were employed to determine the significance of environmental effects. Our results demonstrate that a mild hypothermic condition of 18 degrees C significantly enhanced the production of eicosapentaenoic acid (EPA) compared to higher temperatures. Conversely, cell density was maximized at 22 degrees C. Under the 18 degrees C thermal regime, lower light intensities (100-185 & micro;mol photons m-2 s-1) promoted a superior synthesis of both bioactive lipids and pigments. In conclusion, the strategic application of mild hypothermia combined with moderate light intensity is an effective approach to substantially boost the metabolic yield of high-value compounds in R. marinus, highlighting its potential as a sustainable source for nutraceutical and pharmaceutical applications.

0026-01-01T00:00:00Z