http://scholars.ntou.edu.tw/handle/123456789/205 Wed, 08 Apr 2026 13:39:19 GMT 2026-04-08T13:39:19Z http://scholars.ntou.edu.tw:80/retrieve/87/海洋生物科技學士學位學程(系).png http://scholars.ntou.edu.tw/handle/123456789/205 http://scholars.ntou.edu.tw/handle/123456789/26526 標題: CFOs with social connections to audit committees and internal control quality 作者: Lai, Shu-Miao; Liu, Chih-Liang; Chen, Sheng-Syan 摘要: This study examines how chief financial officers' (CFOs) social connections to the audit committee impact the quality of internal control over financial reporting (ICFR). Using restatements to identify firms with potential internal control weaknesses (ICWs) during restatement periods, we find that CFOs' social connections to the audit committee are less likely to lead to disclosure of potential ICWs; instead, they are more likely to lead to reporting of potential ICWs for irregularity restatements. Firms with CFOs' social connections to the audit committee exhibit higher financial reporting quality and reputational capital. The negative relation between the CFOs' social connections to the audit committee and disclosure of potential ICWs is more pronounced for CFOs with higher reputational capital. Overall, this research elucidates how CFOs' social connections to the audit committee can facilitate information exchange, enabling the CFO and the audit committee to effectively collaborate and monitor ICFR, which in turn diminishes the likelihood of potential ICWs occurring during restatement periods. Thu, 01 Jan 2026 00:00:00 GMT http://scholars.ntou.edu.tw/handle/123456789/26526 2026-01-01T00:00:00Z http://scholars.ntou.edu.tw/handle/123456789/26393 標題: Commercial probiotics enhance growth and immunity of white shrimp (Litopenaeus vannamei) and resistence to infection by Vibrio parahaemolyticus 作者: Maurus, Germain; Joe, Joan Tang Xiao; Phum, Quoc Hung; Lin, Shih-Chao; Lu, Ming-Wei 摘要: This study evaluated the benefits of a commercial probiotic, Aqua Fortuna probiotic (AFPB), containing multiple bacterial strains isolated from fish gut microbiota, on the physiological performance of white-leg shrimp (Litopenaeus vannamei). Juvenile shrimp were fed varying doses of AFPB (0.5%, 1%, 2%, and 5%) during their maturation period, and their growth indices, including weight, growth rate, feed conversion ratio, and survival rate, were measured biweekly. In addition, gene expression levels of growth factors (IGF1, IGFBP, VEGF1-3) and immune-related markers (antimicrobial peptides: penaeidin-3a, crustin, lysozyme; pattern recognition receptors: beta-1,3-glucan binding protein, lipopolysaccharide, and beta-glucan binding protein; detoxification enzymes: SOD, prophenoloxidase, hemocyanin) were analyzed in digestive tissues via qPCR on week 8. Furthermore, L. vannamei was challenged with Vibrio parahaemolyticus, and post-challenge survival rates were monitored. The results revealed that AFPB supplementation significantly enhanced the growth performance of L. vannamei. Notably, 1% of AFPB yielded the most significant improvements in the final weights, specific growth rates, and better survival rates. During the lethal challenge with V. parahaemolyticus, groups treated with 5% and 2% AFPB showed higher survival rates at 4 and 8 weeks, respectively. Gene expression analyses showed that 1% AFPB upregulated penaeidin-3a, crustin, lysozyme, prophenoloxidase, superoxide dismutase, and beta-1, 3 glucans binding protein in digestive tissues, whereas 2% AFPB additionally upregulated immune genes like prophenoloxidase and lysozyme. Our findings highlight the role of shrimp microbiota in promoting growth and enhancing disease resistance, providing robust evidence for the use of probiotics as a strategy to improve shrimp cultivation productivity. Wed, 01 Jan 2025 00:00:00 GMT http://scholars.ntou.edu.tw/handle/123456789/26393 2025-01-01T00:00:00Z http://scholars.ntou.edu.tw/handle/123456789/26331 標題: A psychiatric medication, clozapine, induces autophagy and apoptosis in breast cancer cells through reactive oxygen species 作者: Fan, Ya-Chun; Lin, Shih-Chao; Lai, Po-Jung; Lai, Pei-Chun; Maurus, Germain; Chen, Shiow-Yi 摘要: Cancer patients with psychotic disorders have occasionally exhibited reduced tumor sizes following long-term antipsychotic treatment. Previous studies have shown that antipsychotic drugs, such as clozapine, could inhibit cancer cell proliferation, but the underlying mechanisms remain unclear. This study investigates the anti-tumor effects of clozapine on breast cancer cells and explores its mechanisms of action. We used clonogenic and MTT assays to assess cell proliferation, flow cytometry and western blotting analyses to evaluate cell cycle distribution, apoptosis, and autophagy following clozapine exposure. The results show that clozapine downregulates Cyclin D1, CDK4, and CDK6, while upregulating p21 and p27 in MCF-7 cells, leading to G0/G1 phase arrest. Clozapine exposure also increases reactive oxygen species (ROS), apoptosis and autophagy levels. Notably, treatment with the antioxidant alpha-Tocopherol restores cell viability and reduces ROS and autophagy, indicating that ROS plays a central role in clozapine-induced cytotoxicity. Additionally, inhibition of autophagy using chloroquine enhances clozapine-induced apoptosis and further reduces cell viability. These findings suggest that clozapine induces apoptosis and autophagy through ROS generation and that combining clozapine with autophagy inhibitors could sensitize MCF-7 cells to treatment. Furthermore, clozapine induces significant cytotoxicity in MDA-MB-231 cells, an aggressive, ER-negative breast cancer model, through similar ROS- and autophagy-mediated mechanisms. The addition of alpha-Tocopherol similarly rescued these cells from clozapine-induced cell death. Overall, our study demonstrates that clozapine suppresses the growth of both MCF-7 and MDA-MB-231 breast cancer cells by inducing cytotoxicity via ROS and autophagy, highlighting its potential as a therapeutic agent, especially in combination with autophagy inhibitors. Wed, 01 Jan 2025 00:00:00 GMT http://scholars.ntou.edu.tw/handle/123456789/26331 2025-01-01T00:00:00Z http://scholars.ntou.edu.tw/handle/123456789/26299 標題: Transcriptional responses to nitrogen and phosphorus costress conditions reveal nitrogen-dominant control in a marine diatom, Skeletonema tropicum 作者: Shih, Chi-Yu; Chuang, Chih-Yu; Chang, Jeng; Gong, Gwo-Ching; Kang, Lee-Kuo 摘要: To elucidate the responses of phytoplankton to combined nitrogen (N) and phosphorus (P) stress, we conducted a full factorial experiment to investigate the transcriptional regulation of nutrient-responsive genes in the diatom Skeletonema tropicum. This study aimed to characterize how nutrient availability and physiological status modulate gene expression, with a particular emphasis on the interplay between N and P in shaping transcriptional responses. A matrix of N/P combinations was employed to simulate diverse nutrient regimes consistent with the theoretical framework of nutrient colimitation. The expression of the nitrate transporter gene (Nrt2) was strongly repressed in the presence of ammonium but highly induced following ammonium depletion, demonstrating an inverse relationship with ammonium concentrations in the medium and highlighting direct regulation by nitrogen availability. In contrast, the expression of phosphorus-responsive genes, including phosphate transporters (Pho and Npt2bl) and alkaline phosphatase (Ap1), was upregulated under P deficiency, yet their expression was also modulated by ammonium concentrations and the N:P ratio of the medium. Under combined N and P stress, S. tropicum prioritizes nitrogen acquisition, suggesting a regulatory hierarchy between N and P uptake. The results highlight the complex transcriptional strategies that enable diatoms to adapt to fluctuating nutrient regimes in marine environments. Thu, 01 Jan 2026 00:00:00 GMT http://scholars.ntou.edu.tw/handle/123456789/26299 2026-01-01T00:00:00Z