http://scholars.ntou.edu.tw/handle/123456789/207 Wed, 08 Apr 2026 13:39:19 GMT 2026-04-08T13:39:19Z http://scholars.ntou.edu.tw:80/retrieve/89/食品安全與風險管理研究所.jpg http://scholars.ntou.edu.tw/handle/123456789/207 http://scholars.ntou.edu.tw/handle/123456789/26547 標題: Maximizing beta-carotene production from Dunaliella salina using different concentrations of ferrous sulfate and potassium nitrate under in situ and induced cultivation conditions 作者: Yeh, Han-Yang; Yu, Po-Yen; Lee, Meng-Chou; Ching,Congo Tak Shing; Nan, Fan-Hua; Yao, Chao-Ling; Lin, Yung-Kai 摘要: Natural beta-carotene, a valuable antioxidant and food additive, is conventionally derived from vegetables and microalgae such as Dunaliella salina (D. salina). Growing concerns over the safety of synthetic carotenoids and increasing demand for natural alternatives in food, nutraceutical, and cosmetic industries have accelerated the development of controlled cultivation systems. However, unstable environmental conditions in outdoor production systems can hinder consistent carotenoid yields. In this context, D. salina is recognized as a promising candidate for beta-carotene production through indoor cultivation, which allows for precise control over key growth parameters and reduces dependency on fluctuating environmental factors. This study investigated how nitrogen availability and ferrous ion supplementation affect the growth and beta-carotene accumulation of D. salina under both in situ and stress-induced conditions. Results show that nitrogen supports robust biomass accumulation, while ferrous ions stimulate beta-carotene synthesis via oxidative stress. Notably, the combined application of these two nutrients produced a synergistic effect, achieving both high cell density (2.4079 +/- 0.00432 x 107 cells mL-1) and elevated beta-carotene content (27.12 +/- 1.41 pg cell-1) after 20 days of induced two-stage cultivation in a 15 L indoor culture system. These findings contribute to the development of optimized cultivation strategies for sustainable, high-yield beta-carotene production. Wed, 01 Jan 2025 00:00:00 GMT http://scholars.ntou.edu.tw/handle/123456789/26547 2025-01-01T00:00:00Z http://scholars.ntou.edu.tw/handle/123456789/26515 標題: Biomarker-Based Risk Assessment Strategy for Long COVID: Leveraging Spike Protein and Proinflammatory Mediators to Inform Broader Postinfection Sequelae 作者: Yang, Ying-Fei; Ling, Min-Pei; Chen, Szu-Chieh; Lin, Yi-Jun; You, Shu-Han; Lu, Tien-Hsuan; Chen, Chi-Yun; Wang, Wei-Min; Chen, Si-Yu; Lai, I-Hsuan; Hsiao, Huai-An; Liao, Chung-Min 摘要: Long COVID, characterized by persistent symptoms following acute SARS-CoV-2 infection, has emerged as a significant public health challenge with wide-ranging clinical and socioeconomic implications. Developing an effective risk assessment strategy is essential for the early identification and management of individuals susceptible to prolonged symptoms. This study uses a quantitative approach to characterize the dose-response relationships between spike protein concentrations and effects, including Long COVID symptom numbers and the release of proinflammatory mediators. A mathematical model is also developed to describe the time-dependent change in spike protein concentrations post diagnosis in twelve Long COVID patients with a cluster analysis. Based on the spike protein concentration-Long COVID symptom numbers relationship, we estimated a maximum symptom number (similar to 20) that can be used to reflect a persistent predictor. We found that among the crucial biomarkers associated with Long COVID proinflammatory mediator, CXCL8 has the lowest 50% effective dose (0.01 mu g mL(-1)), followed by IL-6 (0.39), IL-1 beta (0.46), and TNF-alpha (0.56). This work provides a comprehensive risk assessment strategy with dose-response tools and mathematical modeling developed to estimate potential spike protein concentration. Our study suggests persistent Long COVID guidelines for personalized care strategies and could inform public health policies to support early interventions that reduce long-term disability and healthcare burdens with possible other post-infection syndromes. Wed, 01 Jan 2025 00:00:00 GMT http://scholars.ntou.edu.tw/handle/123456789/26515 2025-01-01T00:00:00Z http://scholars.ntou.edu.tw/handle/123456789/26402 標題: Optimized Microbial Scaffolds Immobilized with Pleurotus ostreatus and Aspergillus oryzae on Foaming Bacterial Cellulose 作者: Chan, Pei-Ching; Ku, Wei-Lun; Chuang, Yung-Kun; Chou, Yu-Chieh; Hsieh, Chen-Che; Lin, Yung-Kai; Santoso, Shella Permatasari; Lin, Shin-Ping 摘要: In this study, we explored the development and characterization of fungus-immobilized foamed bacterial cellulose (FBC) scaffolds using Pleurotus ostreatus and Aspergillus oryzae. FBC, a porous biomaterial with high structural integrity and resistance to enzymatic degradation, served as a three-dimensional matrix for fungal cultivation. The results indicated effective fungal immobilization, with the 1% A. oryzae-immobilized FBC group (FBC/1A) achieving the highest production yield. The water content (97%) and swelling behavior (95.9%) analyses revealed that P. ostreatus-immobilized FBC maintained high hydration levels and rehydration capacities, whereas A. oryzae immobilization led to slightly reduced water retention. Morphological assessments via SEM confirmed the presence of fungal-derived fibers integrated with native cellulose structures, suggesting successful immobilization. A thermogravimetric analysis demonstrated enhanced thermal stability in fungus-immobilized FBC, particularly in the A. oryzae group, while FTIR spectra suggested possible structural alterations induced by fungal activity. Collectively, these findings support the potential of fungal-immobilized FBC as a robust, biodegradable material with promising applications in biotechnology and sustainable material development. Wed, 01 Jan 2025 00:00:00 GMT http://scholars.ntou.edu.tw/handle/123456789/26402 2025-01-01T00:00:00Z http://scholars.ntou.edu.tw/handle/123456789/26397 標題: Bacillus coagulans TCI803 confers gastroesophageal protection against Helicobacter pylori-evoked gastric oxidative stress and acid-induced lower esophageal sphincter inflammation 作者: Cheng, Yu-Hsuan; Li, Hung-Keng; Chang, Kai-Hsian; Lin, Yung-Kai; Lin, Yung-Hsiang; Chiang, Chi-Fu; Yang, Jyh-Chin; Chien, Chiang-Ting 摘要: Background:Probiotic Bacillus coagulans (BC) may have an impact on gastrointestinal protection. This study was designed to investigate the BC effects on Helicobacter pylori (H. pylori) induced gastric inflammation in mice and acid-induced lower esophageal sphincter (LES) dysfunction in rats. We determined the oxidative stress/apoptosis/autophagy signaling pathways in H. pylori-induced gastric inflammation and HCl-evoked LES inflammation.Methods:H. pylori-induced gastric inflammation was used in 40 mice and HCl-evoked LES inflammation in 40 Wistar rats. Western blot, immunohistochemistry and cytokine array were used to determine the pathophysiologic mechanisms.Results:H. pylori increased leukocyte infiltration-mediated inflammation and the expression levels of gastric cytokines, 3NT/4HNE-mediated oxidative stress, and Bax/Caspase3-mediated apoptosis, but decreased Beclin-1/LC3-II-mediated autophagy in the mice gastric mucosa. BC treatment decreased inflammation, cytokines release, oxidative stress, and apoptosis, and reversed autophagy in H. pylori-infected gastric mucosa. To replace the antibiotic therapy, BC TCI803 was selected to inhibit H. pylori infection for commercial interests. Saline esophageal infusion evoked an increase in LES pressure and efferent vagus nerve activity during the emptying phase. However, HCI dysregulated LES motility esophageal infusion by a decrease in threshold pressure, intercontraction interval and an increase in efferent vagus nerve activity. BC treatment significantly recovered the level of threshold pressure, intercontraction interval, and depressed the enhanced efferent vagus nerve activity. In vitro LES wire myography data displayed that HCl-treated LES significantly decreased the contractile response to acetylcholine. BC treatment significantly restored the contractile response to acetylcholine in LES wire myography. LES after HCl stimulation significantly increased leukocyte infiltration-mediated inflammation, whereas BC treatment effectively reduced the leukocyte infiltration-mediated inflammation in the HCl-treated LES.Conclusion:BC via anti-oxidation and anti-inflammation confers gastroesophageal protection against H. pylori involved oxidative stress/inflammation/apoptosis/autophagy signaling in mice with gastric inflammation and HCl-induced LES dysregulation and inflammation. Wed, 01 Jan 2025 00:00:00 GMT http://scholars.ntou.edu.tw/handle/123456789/26397 2025-01-01T00:00:00Z