http://scholars.ntou.edu.tw/handle/123456789/208 Wed, 08 Apr 2026 10:06:54 GMT 2026-04-08T10:06:54Z https://scholars.ntou.edu.tw:443/retrieve/90/食品科學系.jpg http://scholars.ntou.edu.tw/handle/123456789/208 http://scholars.ntou.edu.tw/handle/123456789/26549 標題: Enhanced Production of Bioactive Polyunsaturated Fatty Acids and Pigments in Rhodosorus marinus: Optimization of Thermal and Photic Stress Conditions 作者: Cheng, Wen-Ping; Yeh, Han-Yang; Chen, Yen-Ling; Chen, Yi-Jung; Sze, Fat-Tin Agassi; Huang, Chi-Cheng; Nan, Fan-Hua; Fang, Ming-Chih; Lee, Meng-Chou 摘要: The marine unicellular red alga Rhodosorus marinus is a promising source of the valuable phycobiliprotein phycoerythrin and essential omega-3 polyunsaturated fatty acids (PUFAs), yet the environmental triggers for their optimal co-production remain to be fully elucidated. This study was conducted to investigate the effects of thermal and photic stress in terms of maximizing the yield of these high-value bioactive compounds. R. marinus was cultivated under a range of temperatures (18-24 degrees C) and light intensities (100-335 & micro;mol photons m-2 s-1) to assess its physiological and biochemical responses, particularly focusing on lipid accumulation. This study investigates the effects of thermal (18-24 degrees C) and photic (100-335 & micro;mol photons m-2 s-1) stress on the concurrent production of the valuable phycobiliprotein, phycoerythrin (PE), and essential omega-3 polyunsaturated fatty acids (PUFAs) in the marine red microalga Rhodosorus marinus. Fatty acid profiles were quantified using gas chromatography (GC), while pigment content was assessed via spectrophotometry. Statistical analyses, including one-way ANOVA and Tukey's post hoc test, were employed to determine the significance of environmental effects. Our results demonstrate that a mild hypothermic condition of 18 degrees C significantly enhanced the production of eicosapentaenoic acid (EPA) compared to higher temperatures. Conversely, cell density was maximized at 22 degrees C. Under the 18 degrees C thermal regime, lower light intensities (100-185 & micro;mol photons m-2 s-1) promoted a superior synthesis of both bioactive lipids and pigments. In conclusion, the strategic application of mild hypothermia combined with moderate light intensity is an effective approach to substantially boost the metabolic yield of high-value compounds in R. marinus, highlighting its potential as a sustainable source for nutraceutical and pharmaceutical applications. Tue, 01 Jan 0026 00:00:00 GMT http://scholars.ntou.edu.tw/handle/123456789/26549 0026-01-01T00:00:00Z http://scholars.ntou.edu.tw/handle/123456789/26542 標題: Ulva polysaccharides-based nanoemulsion hydrogel for enhanced skin delivery and protection against UVB-induced damage 作者: Fang, Shih-Yuan; Chan, Hsiang-Wen; Fang, Ming-Chih; Huang, Chung-Hsiung 摘要: Ultraviolet (UV)B exposure contributes significantly to skin injury by increasing the generation of reactive oxygen species (ROS), resulting in keratinocyte damage, collagen degradation, inflammation, and premature aging. Current therapeutic approaches are often invasive and associated with undesirable side effects, highlighting the importance of safer, non-invasive alternatives. In this study, a nanoemulsion (NE) was developed using Ulva polysaccharides (UP) as the active ingredient and squalane as a biocompatible oil phase, followed by incorporation into a 0.5% Carbopol hydrogel (NE@hydrogel) for topical application. The NE was prepared via homogenization and ultrasonic treatment, yielding particles with a mean size of 63.8 nm and exhibiting good stability for at least 8 weeks. Skin permeation studies confirmed enhanced dermal delivery, with approximately 21% for hydrophilic and 66% for lipophilic compounds. In vitro, UVB exposure (120 mJ/cm2) lowered HaCaT cell survival with concomitant rise in intracellular ROS, whereas treatment with 10 & times; diluted NE restored cell viability to approximately 74%, reduced ROS levels, and promoted cell migration to 66% at 24 h in the wound healing assay. In vivo, NE@hydrogel application mitigated UVB-induced epidermal thickening from approximately 88 mu m to 50 mu m and preserved epidermal architecture by reducing histological alterations. These results demonstrate that the NE@hydrogel exhibit potent antioxidant and skin-repairing activities, highlighting their potential as natural, non-invasive topical formulations for preventing and alleviating acute UVB-induced skin injury. Thu, 01 Jan 2026 00:00:00 GMT http://scholars.ntou.edu.tw/handle/123456789/26542 2026-01-01T00:00:00Z http://scholars.ntou.edu.tw/handle/123456789/26541 標題: Comparative immunomodulatory and gut microbiota-regulating effects of macroalgal polysaccharides in cyclophosphamide-induced immunosuppressed mice 作者: Huang, Wei-Hao; Tsai, Pin-Yu; Chen, Chien-Li; Huang, Chung-Hsiung 摘要: Polysaccharides derived from macroalgae exhibit potent immunomodulatory and gut-regulatory activities; however, comprehensive comparisons of their differential bioactivities remain scarce. This study systematically evaluated the immunomodulatory potential of structurally distinct polysaccharides extracted from Sargassum fusiforme (SP) and Ulva lactuca (UP) in a cyclophosphamide (CTX)-induced immunosuppressed mouse model. Structural analyses revealed that SP consisted mainly of fucose (64.5%) and galactose (20.4%) with molecular masses of 95.6 and 1.4 kDa, while UP was primarily composed of glucose (60.4%) and rhamnose (34.1%) with molecular masses of 908.2, 10.9, and 1.2 kDa. Both polysaccharides exhibited characteristic sulfate absorption peaks. Oral administration of SP or UP (125 and 250 mg/mL) for 14 days significantly alleviated CTX-induced reductions in thymus and spleen indices and mitigated lymphoid tissue injury. Treatments restored splenocyte populations-including CD8+ T cells and B220+ B cells-increased serum IgG levels and enhanced splenocyte viability. SP and UP also promoted lipopolysaccharide (LPS)-induced cytokine production, notably IL-2, IFN-gamma, IL-6, and TNF-alpha, with SP showing stronger immunostimulatory effects, particularly in B-cell restoration and cytokine secretion. Gut microbiota profiling revealed that both polysaccharides ameliorated CTX-induced dysbiosis by enriching Lactobacillus, reducing Desulfovibrio, and increasing short-chain fatty acid levels. Collectively, these findings demonstrate that SP and UP enhance immune function and modulate gut microbiota in immunosuppressed mice, with SP exerting superior activity. This first comparative study provides mechanistic insight and supports their potential as functional food supplements or immunotherapeutic adjuvants. Thu, 01 Jan 2026 00:00:00 GMT http://scholars.ntou.edu.tw/handle/123456789/26541 2026-01-01T00:00:00Z http://scholars.ntou.edu.tw/handle/123456789/26529 標題: Targeted Lipidomics and Lipid Metabolism Elucidate Anti-Obesity Effects of Lactic Acid Bacteria-Fermented Purple Sweet Potato Tainung No. 73 Extract in Obese Mice 作者: Yang, Hsien-Yi; Huang, Chien-Hsun; Ho, Shang-Tse; Su, Hsin-Hui; Chen, Yen-Po; Chen, Yung-Tsung 摘要: The increasing prevalence of obesity and metabolic disorders poses a major global health challenge. In the present study, purple sweet potato Tainung No. 73 was fermented using Lactobacillus amylovorus OFMLa-73 and Levilactobacillus brevis OFMLb-143 to enrich the specific bioactive metabolite indolelactic acid. Furthermore, supplementation with fermented sweet potato (FSPE) ethanol extract resulted in a significant reduction in body weight gain, adipocyte hypertrophy, and hepatic lipid accumulation, while also improving serum lipid profiles in high-fat diet-induced obesity mice. These physiological improvements were associated with the downregulated expression of adipogenic and inflammatory genes in both liver and adipose tissues. Furthermore, lipidomic analysis revealed that FSPE modulated key lipid species, including ceramides and acylcarnitines, which are implicated in metabolic dysfunction. Collectively, these findings demonstrated that lactic acid fermentation enhanced purple sweet potato's functional potential, positioning FSPE as a promising candidate for dietary intervention in obesity management. Thu, 01 Jan 2026 00:00:00 GMT http://scholars.ntou.edu.tw/handle/123456789/26529 2026-01-01T00:00:00Z