http://scholars.ntou.edu.tw/handle/123456789/14465
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Han YS | en_US |
dc.contributor.author | I-Chiu Liao | en_US |
dc.contributor.author | Huang YS | en_US |
dc.contributor.author | Tzeng WN | en_US |
dc.contributor.author | Yu JY. | en_US |
dc.date.accessioned | 2020-12-04T07:56:00Z | - |
dc.date.available | 2020-12-04T07:56:00Z | - |
dc.date.issued | 2003-08 | - |
dc.identifier.uri | http://ntour.ntou.edu.tw:8080/ir/handle/987654321/45062 | - |
dc.identifier.uri | http://scholars.ntou.edu.tw/handle/123456789/14465 | - |
dc.description.abstract | The complete complementary DNA (cDNA) encoding pituitary gonadotropin II-beta subunit (GTH II-beta) of Japanese eel Anguilla japonica was cloned and sequenced, and the profiles of pituitary glycoprotein hormone alpha subunit (PGH-alpha), GTH I-beta, and GTH II-beta mRNA transcript levels at different stages of ovarian development before vitellogenesis in the wild females were investigated. The maturity of female eels was divided into four stages: juvenile, sub-adult, pre-silver, and silver stages based on ovarian development and skin color. The GTH II-beta cDNA was cloned by reverse transcription and polymerase chain reaction (RT-PCR) amplification from total pituitary RNA. The full length GTH II-beta cDNA was obtained using 5(')- and 3(')-rapid amplification of cDNA ends. The cloned eel GTH II-beta cDNA consists of 646 bp nucleotides, including 53 bp nucleotides of 5(')-untranslated region (UTR), 423 bp of open reading frame, and 170 bp nucleotides of 3(')-UTR followed by a poly(A) tail. It encodes a 140-amino acid precursor molecule of GTH II-beta subunit with a putative signal peptide of 24 amino acids and a mature peptide of 116 amino acids. RT-PCR analysis showed that the pituitary transcript levels of alpha subunit steadily increased during eel silvering. The expression of GTH I-beta and II-beta mRNA levels, however, varied in different ovarian developmental stages. The mRNA expression of both GTH I-beta and GTH II-beta were detectable in juvenile stage. The expression levels of GTH II-beta mRNA, but not GTH I-beta, were significantly increased in sub-adult stage. The transcript levels of GTH I-beta and II-beta subunits further increased in pre-silver and silver stages. We demonstrated for the first time that the differential transcription patterns of pituitary PGH-alpha, GTH I-beta, and GTH II-beta mRNAs occur during silvering of the wild female Japanese eels. | en_US |
dc.language.iso | en | en_US |
dc.publisher | General and Comparative Endocrinology | en_US |
dc.title | Profiles of PGH-α, GTH I-β, and GTH II-β mRNA transcript levels at different ovarian stages in the wild female Japanese eel Anguilla japonica | en_US |
dc.type | journal article | en_US |
dc.identifier.doi | 10.1016/s0016-6480(03)00143-6 | - |
dc.identifier.pmid | 12899842 | - |
dc.relation.journalvolume | 133 | en_US |
dc.relation.journalissue | 1 | en_US |
dc.relation.pages | pp.8-16 | en_US |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
crisitem.author.dept | Center of Excellence for the Oceans | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
顯示於: | 海洋中心 |
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