http://scholars.ntou.edu.tw/handle/123456789/19546
標題: | Identified Seaweed Compound Diphenylmethane Serves as an Efflux Pump Inhibitor in Drug-Resistant Escherichia coli | 作者: | Lu, Wen-Jung Hsu, Pang-Hung Chang, Chun-Ju Su, Cheng-Kuan Huang, Yan-Jyun Lin, Hsuan-Ju Lai, Margaret Ooi, Gui-Xia Dai, Jing-Yi Lin, Hong-Ting Victor |
關鍵字: | seaweeds;efflux pump inhibitors;multidrug resistance;drug transporters;diphenylmethane;aromatic compounds | 公開日期: | 1-十一月-2021 | 出版社: | MDPI | 卷: | 10 | 期: | 11 | 來源出版物: | ANTIBIOTICS-BASEL | 摘要: | Drug efflux pumps are one of the major elements used by antibiotic-resistant bacteria. Efflux pump inhibitors (EPIs) are potential therapeutic agents for adjunctive therapy, which can restore the activity of antibiotics that are no longer effective against pathogens. This study evaluated the seaweed compound diphenylmethane (DPM) for its EPI activity. The IC50 and modulation results showed that DPM has no antibacterial activity but can potentiate the activity of antibiotics against drug-resistant E. coli. Time-kill studies reported that a combination of DPM and erythromycin exhibited greater inhibitory activity against drug-resistant Escherichia coli. Dye accumulation and dye efflux studies using Hoechst 33342 and ethidium bromide showed that the addition of DPM significantly increased dye accumulation and reduced dye efflux in drug-resistant E. coli, suggesting its interference with dye translocation by an efflux pump. Using MALDI-TOF, it was observed that the addition of DPM could continuously reduce antibiotic efflux in drug-resistant E. coli. Additionally, DPM did not seem to damage the E. coli membranes, and the cell toxicity test showed that it features mild human-cell toxicity. In conclusion, these findings showed that DPM could serve as a potential EPI for drug-resistant E. coli. |
URI: | http://scholars.ntou.edu.tw/handle/123456789/19546 | ISSN: | 2079-6382 | DOI: | 10.3390/antibiotics10111378 |
顯示於: | 生命科學暨生物科技學系 食品科學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。