http://scholars.ntou.edu.tw/handle/123456789/19565
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Yen, Shao-Chieh | en_US |
dc.contributor.author | Mao, Ju-Yi | en_US |
dc.contributor.author | Lin, Hung-Yun | en_US |
dc.contributor.author | Huang, Huai-Ting | en_US |
dc.contributor.author | Harroun, Scott G. | en_US |
dc.contributor.author | Nain, Amit | en_US |
dc.contributor.author | Chang, Huan-Tsung | en_US |
dc.contributor.author | Lin, Han-You | en_US |
dc.contributor.author | Chen, Li-Li | en_US |
dc.contributor.author | Huang, Chih-Ching | en_US |
dc.contributor.author | Lin, Han-Jia | en_US |
dc.date.accessioned | 2022-01-03T02:20:19Z | - |
dc.date.available | 2022-01-03T02:20:19Z | - |
dc.date.issued | 2021-12-24 | - |
dc.identifier.issn | 1477-3155 | - |
dc.identifier.uri | http://scholars.ntou.edu.tw/handle/123456789/19565 | - |
dc.description.abstract | Background: Shrimp aquaculture has suffered huge economic losses over the past decade due to the outbreak of acute hepatopancreatic necrosis disease (AHPND), which is mainly caused by the bacteria Vibrio parahaemolyticus (V. parahaemolyticus) with the virulence pVA1 plasmid, which encodes a secretory photorhabdus insect-related (Pir) toxin composed of PirA and PirB proteins. The Pir toxin mainly attacks the hepatopancreas, a major metabolic organ in shrimp, thereby causing necrosis and loss of function. The pandemic of antibiotic-resistant strains makes the impact worse. Methods: Mild pyrolysis of a mixture of polysaccharide dextran 70 and the crosslinker 1,8-diaminooctane at 180 degrees C for 3 h to form carbonized nanogels (DAO/DEX-CNGs) through controlled cross-linking and carbonization. The multi-functional therapeutic CNGs inherit nanogel-like structures and functional groups from their precursor molecules. Results: DAO/DEX-CNGs manifest broad-spectrum antibacterial activity against Vibrio parahaemolyticus responsible for AHPND and even multiple drug-resistant strains. The polymer-like structures and functional groups on graphitic-carbon within the CNGs exhibit multiple treatment effects, including disruption of bacterial membranes, elevating bacterial oxidative stress, and neutralization of PirAB toxins. The inhibition of Vibrio in the midgut of infected shrimp, protection of hepatopancreas tissue from Pir toxin, and suppressing overstimulation of the immune system in severe V parahaemolyticus infection, revealing that CNGs can effectively guard shrimp from Vibrio invasion. Moreover, shrimps fed with DAO/DEX-CNGs were carefully examined, such as the expression of the immune-related genes, hepatopancreas biopsy, and intestinal microbiota. Few adverse effects on shrimps were observed. Conclusion: Our work proposes brand-new applications of multifunctional carbon-based nanomaterials as efficient anti-Vibrio agents in the aquatic industry that hold great potential as feed additives to reduce antibiotic overuse in aquaculture. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | BMC | en_US |
dc.relation.ispartof | J NANOBIOTECHNOL | en_US |
dc.subject | PACIFIC WHITE SHRIMP | en_US |
dc.subject | LITOPENAEUS-VANNAMEI | en_US |
dc.subject | QUANTUM DOTS | en_US |
dc.subject | VIBRIO-PARAHAEMOLYTICUS | en_US |
dc.subject | POTENTIAL APPLICATION | en_US |
dc.subject | FOOD ANIMALS | en_US |
dc.subject | NANOPARTICLES | en_US |
dc.subject | AHPND | en_US |
dc.subject | SPERMIDINE | en_US |
dc.subject | IMPACTS | en_US |
dc.title | Multifunctional carbonized nanogels to treat lethal acute hepatopancreatic necrosis disease | en_US |
dc.type | journal article | en_US |
dc.identifier.doi | 10.1186/s12951-021-01194-8 | - |
dc.identifier.isi | WOS:000734031900002 | - |
dc.relation.journalvolume | 19 | en_US |
dc.relation.journalissue | 1 | en_US |
item.languageiso639-1 | en_US | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Institute of Marine Biology | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | Doctoral Degree Program in Marine Biotechnology | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Department of Bioscience and Biotechnology | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | Center of Excellence for the Oceans | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Department of Bioscience and Biotechnology | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | Bachelor Degree Program in Marine Biotechnology | - |
crisitem.author.dept | Center of Excellence for the Oceans | - |
crisitem.author.orcid | 0000-0002-9593-8004 | - |
crisitem.author.orcid | 0000-0002-0363-1129 | - |
crisitem.author.orcid | 0000-0002-4929-6573 | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
顯示於: | 海洋生物研究所 生命科學暨生物科技學系 03 GOOD HEALTH AND WELL-BEING 14 LIFE BELOW WATER |
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