http://scholars.ntou.edu.tw/handle/123456789/20428
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Lin, Yu-Hsin | - |
dc.contributor.author | Chen, Guan-Wen | - |
dc.contributor.author | Yeh, Chin Hsi | - |
dc.contributor.author | Song, Helena | - |
dc.contributor.author | Tsai, Jenn-Shou | - |
dc.date.accessioned | 2022-02-17T03:56:24Z | - |
dc.date.available | 2022-02-17T03:56:24Z | - |
dc.date.issued | 2018-10 | - |
dc.identifier.issn | 2072-6643 | - |
dc.identifier.uri | http://scholars.ntou.edu.tw/handle/123456789/20428 | - |
dc.description.abstract | Hot water was used to obtain Chlorella sorokiniana hot water extract (HWE). Subsequently, this byproduct was freeze-dried, hydrolysed at 50 degrees C using Protease N to obtain C. sorokiniana protein hydrolysates (PN-1), and then digested with a gastrointestinal enzyme (PN-1G). The inhibitory effects of the HWE and hydrolysates against angiotensin I-converting enzyme (ACE) were investigated. The soluble protein and peptide contents were 379.9 and 179.7 mg/g, respectively, for HWE and 574.8 and 332.8 mg/g, respectively, for PN-1. The IC50 values of the HWE, PN-1, and PN-1G on ACE were 1.070, 0.035, and 0.044 mg/mL, respectively. PN-1G was separated into seven fractions through size exclusion chromatography. The sixth fraction of the hydrolysate had a molecular weight between 270 and 340 Da, and the lowest IC50 value on ACE was 0.015 mg/mL. The amino acid sequences of the ACE-inhibitory peptides were Trp-Val, Val-Trp, Ile-Trp, and Leu-Trp, of which the IC50 values were 307.61, 0.58, 0.50, and 1.11 mu M, respectively. Systolic blood pressure and diastolic blood pressure were reduced 20 and 21 mm Hg, respectively, in spontaneously hypertensive rats after 6 h of oral administration with a dose of 171.4 mg PN-1 powder/kg body weight. | - |
dc.language.iso | en_US | - |
dc.publisher | MDPI | - |
dc.relation.ispartof | NUTRIENTS | - |
dc.subject | WAKAME UNDARIA-PINNATIFIDA | - |
dc.subject | SPONTANEOUSLY HYPERTENSIVE-RATS | - |
dc.subject | SPECTROPHOTOMETRIC ASSAY | - |
dc.subject | ANTIOXIDANT ACTIVITY | - |
dc.subject | SPIRULINA-PLATENSIS | - |
dc.subject | FOOD PROTEINS | - |
dc.subject | SALMON MUSCLE | - |
dc.subject | MILK | - |
dc.subject | DIGEST | - |
dc.subject | FERMENTATION | - |
dc.title | Purification and Identification of Angiotensin I-Converting Enzyme Inhibitory Peptides and the Antihypertensive Effect of Chlorella sorokiniana Protein Hydrolysates | - |
dc.type | journal article | - |
dc.identifier.doi | 10.3390/nu10101397 | - |
dc.identifier.isi | WOS:000448821300063 | - |
dc.identifier.url | <Go to ISI>://WOS:000448821300063 | - |
dc.relation.journalvolume | 10 | - |
dc.relation.journalissue | 10 | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.fulltext | no fulltext | - |
item.languageiso639-1 | en_US | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Department of Food Science | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Department of Food Science | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.orcid | 0000-0002-3435-0725 | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
顯示於: | 食品科學系 03 GOOD HEALTH AND WELL-BEING |
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