http://scholars.ntou.edu.tw/handle/123456789/22498
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Jeng-Jiann Chiu | en_US |
dc.contributor.author | Li-Jing Chen | en_US |
dc.contributor.author | Shun-Fu Chang | en_US |
dc.contributor.author | Pei-Ling Lee | en_US |
dc.contributor.author | Chih-I Lee | en_US |
dc.contributor.author | Min-Chien Tsai | en_US |
dc.contributor.author | Ding-Yu Lee | en_US |
dc.contributor.author | Hsing-Pang Hsieh | en_US |
dc.contributor.author | Shunichi Usami | en_US |
dc.contributor.author | Shu Chien | en_US |
dc.date.accessioned | 2022-10-11T01:32:57Z | - |
dc.date.available | 2022-10-11T01:32:57Z | - |
dc.date.issued | 2005-05 | - |
dc.identifier.issn | 1079-5642 | - |
dc.identifier.uri | http://scholars.ntou.edu.tw/handle/123456789/22498 | - |
dc.description.abstract | Objectives-Vascular endothelial cells (ECs) are influenced by shear stress and neighboring smooth muscle cells (SMCs). We investigated the inflammation-relevant gene expression in EC/SMC cocultures under static condition and in response to shear stress. Materials and Methods-Under static condition, DNA microarrays and reverse-transcription polymerase chain reaction identified 23 inflammation-relevant genes in ECs whose expression was significantly affected by coculture with SMCs, with 18 upregulated and 5 downregulated. Application of shear stress (12 dynes/cm(2)) to the EC side of the coculture for 6 hours inhibited most of the proinflammatory gene expressions in ECs induced by coculture with SMCs. Inhibition of nuclear factor-kappa B (NF-kappa B) activation by the p65-antisense, lactacystin, and N-acetyl-cysteine blocked the coculture-induced EC expression of proinflammatory genes, indicating that the NF-kappa B binding sites in the promoters of these genes play a significant role in their expression as a result of coculture with SMCs. Chromatin immunoprecipitation assays demonstrated the in vivo regulation of NF-kappa B recruitment to selected target promoters. Shear stress inhibited the SMC coculture-induced NF-kappa B activation in ECs and monocytic THP-1 cell adhesion to ECs. Conclusions-Our findings suggest that shear stress plays an inhibitory role in the proinflammatory gene expression in ECs located in close proximity to SMCs. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | LIPPINCOTT WILLIAMS & WILKINS | en_US |
dc.relation.ispartof | ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY | en_US |
dc.subject | COCULTURE MODEL | en_US |
dc.subject | ADHESION | en_US |
dc.subject | PROTEIN | en_US |
dc.subject | VIVO | en_US |
dc.title | Shear stress inhibits smooth muscle cell-induced inflammatory gene expression in endothelial cells: role of NF-kappaB | en_US |
dc.type | journal article | en_US |
dc.identifier.doi | 10.1161/01.ATV.0000159703.43374.19 | - |
dc.identifier.isi | 000228806900371 | - |
dc.relation.journalvolume | 25 | en_US |
dc.relation.journalissue | 5 | en_US |
dc.relation.pages | 963-969 | en_US |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en_US | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Department of Bioscience and Biotechnology | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
顯示於: | 生命科學暨生物科技學系 |
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