http://scholars.ntou.edu.tw/handle/123456789/23799
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Lin, Chia-Yuan | en_US |
dc.contributor.author | Fu, Ru-Huei | en_US |
dc.contributor.author | Chou, Ruey-Hwang | en_US |
dc.contributor.author | Chen, Jing-Hsien | en_US |
dc.contributor.author | Wu, Chi-Rei | en_US |
dc.contributor.author | Chang, Shu-Wei | en_US |
dc.contributor.author | Tsai, Chia-Wen | en_US |
dc.date.accessioned | 2023-05-11T01:35:01Z | - |
dc.date.available | 2023-05-11T01:35:01Z | - |
dc.date.issued | 2017-05 | - |
dc.identifier.issn | 02786915 | - |
dc.identifier.uri | http://scholars.ntou.edu.tw/handle/123456789/23799 | - |
dc.description.abstract | Pi class of glutathione S-transferase (GST) is known to suppress c-Jun N-terminal kinase (JNK)-related apoptosis through protein-protein interactions. Moreover, signaling by PKA/cAMP response element binding protein (CREB) is necessary for GSTP up-regulation. This study explored whether carnosic acid (CA) from rosemary prevents 6-hydroxydopamine (6-OHDA)-induced neurotoxicity by inhibition of JNK through GSTP via PKA/CREB signaling. Results indicated that the GSTP protein was increased in SH-SY5Y cells treated with CA for 18 and 24 h. However, CA had no significant effect on alpha or mu class of GST. Treatment of CA increased the induction of p-PKAα, nuclear p-CREB, and CRE-DNA binding activity. These effects of CA were attenuated in cells pretreated with the PKA inhibitor H89. CA pretreatment suppressed 6-OHDA-induced apoptosis by inhibition of JNK phosphorylation, poly(ADP)-ribose polymerase cleavage, and nuclear condensation. Pretreatment with H89 and GSTP siRNA attenuated the ability of CA to reverse 6-OHDA-induced apoptosis. By use of immunoprecipitation with JNK antibody to examine the interaction of GSTP-JNK with CA, we showed that CA pretreatment increased the immunoprecipitation of GSTP after 6-OHDA treatment, which suggests that CA promoted the interaction between GSTP and JNK. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | ELSEVIER | en_US |
dc.relation.ispartof | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association | en_US |
dc.subject | Carnosic acid;; ; | en_US |
dc.subject | Pi class of glutathione | en_US |
dc.subject | Protein kinase A pathway | en_US |
dc.subject | S-transferase | en_US |
dc.subject | SH-SY5Y cells | en_US |
dc.subject | c-Jun N-Terminal kinase | en_US |
dc.title | Inhibition of JNK by pi class of glutathione S-transferase through PKA/CREB pathway is associated with carnosic acid protection against 6-hydroxydopamine-induced apoptosis | en_US |
dc.type | journal article | en_US |
dc.identifier.doi | 10.1016/j.fct.2017.03.020 | - |
dc.identifier.pmid | 28288932 | - |
dc.identifier.isi | WOS:000399847000019 | - |
dc.relation.journalvolume | 103 | en_US |
dc.relation.pages | 194-202 | en_US |
dc.identifier.eissn | 1873-6351 | - |
item.grantfulltext | none | - |
item.fulltext | no fulltext | - |
item.languageiso639-1 | en_US | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Department of Food Science | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
顯示於: | 食品科學系 |
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