http://scholars.ntou.edu.tw/handle/123456789/23808
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Lin, Chia-Yuan | en_US |
dc.contributor.author | Chen, Jing-Hsien | en_US |
dc.contributor.author | Fu, Ru-Huei | en_US |
dc.contributor.author | Tsai, Chia-Wen | en_US |
dc.date.accessioned | 2023-05-11T03:38:35Z | - |
dc.date.available | 2023-05-11T03:38:35Z | - |
dc.date.issued | 2014-11-17 | - |
dc.identifier.issn | 0893-228X | - |
dc.identifier.issn | 1520-5010 | - |
dc.identifier.uri | http://scholars.ntou.edu.tw/handle/123456789/23808 | - |
dc.description.abstract | Carnosic acid (CA), a diterpene found in the rosemary (Rosmarinus officinalis), has been reported to have a neuroprotective effect. Glutathione S-transferase (GST) P (GSTP) is a phase II detoxifying enzyme that provides a neuroprotective effect. The aim of this study was to explore whether the neuroprotective effect of CA is via an upregulation of GSTP expression and the possible signaling pathways involved. SH-SY5Y cells were pretreated with 1 μM CA followed by treatment with 100 μM 6-hydroxydopamine (6-OHDA). Both immunoblotting and enzyme activity results show that CA also induced protein expression and enzyme activity of GSTP. Moreover, CA significantly increased the phosphorylation of phosphatidylinositol 3-kinase (PI3K)/Akt, the nuclear translocation of p65, but not mitogen-activated protein kinases (p < 0.05). Pretreatment with LY294002 (a PI3K/Akt inhibitor) suppressed the CA-induced phosphorylation of IκB kinase (IKK) and IκBα, p65 nuclear translocation, and nuclear factor-kappa B (NF-κB)-DNA binding activity as well as GSTP protein expression. Furthermore, CA attenuated 6-OHDA-induced caspase 3 activation, and cell death was reversed by GSTP siRNA or LY294002 treatment. Additionally, male Wistar rats with lesions induced by 6-OHDA treatment in the right striatum responded to treatment with CA, which significantly reversed the reduction in GSTP protein expression that resulted from lesioning. We suggest that CA prevents 6-OHDA-induced apoptosis through an increase in GSTP expression via activation of the PI3K/Akt/NF-κB pathway. Therefore, CA may be a promising candidate for use in the prevention of Parkinson's disease. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | AMER CHEMICAL | en_US |
dc.relation.ispartof | Chemical research in toxicology | en_US |
dc.subject | NF-KAPPA-B | en_US |
dc.subject | DOPAMINE-INDUCED APOPTOSIS | en_US |
dc.subject | PARKINSONS-DISEASE | en_US |
dc.subject | CELL-DEATH | en_US |
dc.subject | C-JUN | en_US |
dc.subject | OXIDATIVE STRESS | en_US |
dc.subject | HEME OXYGENASE-1 | en_US |
dc.subject | TERMINAL KINASE | en_US |
dc.subject | EXPRESSION | en_US |
dc.subject | ACTIVATION | en_US |
dc.title | Induction of Pi form of glutathione S-transferase by carnosic acid is mediated through PI3K/Akt/NF-κB pathway and protects against neurotoxicity | en_US |
dc.type | journal article | en_US |
dc.identifier.doi | 10.1021/tx5003063 | - |
dc.identifier.pmid | 25271104 | - |
dc.identifier.isi | WOS:000345264300008 | - |
dc.relation.journalvolume | 27 | en_US |
dc.relation.journalissue | 11 | en_US |
dc.relation.pages | 1958-1966 | en_US |
dc.identifier.eissn | 1520-5010 | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.fulltext | no fulltext | - |
item.languageiso639-1 | en_US | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Department of Food Science | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
顯示於: | 食品科學系 |
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