http://scholars.ntou.edu.tw/handle/123456789/23809
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Chen, Jing-Hsien | en_US |
dc.contributor.author | Ou, Hsin-Ping | en_US |
dc.contributor.author | Lin, Chia-Yuan | en_US |
dc.contributor.author | Lin, Fung-Ju | en_US |
dc.contributor.author | Wu, Chi-Rei | en_US |
dc.contributor.author | Chang, Shu-Wei | en_US |
dc.contributor.author | Tsai, Chia-Wen | en_US |
dc.date.accessioned | 2023-05-11T03:49:15Z | - |
dc.date.available | 2023-05-11T03:49:15Z | - |
dc.date.issued | 2012-09-17 | - |
dc.identifier.issn | 0893-228X | - |
dc.identifier.uri | http://scholars.ntou.edu.tw/handle/123456789/23809 | - |
dc.description.abstract | Understanding the neuroprotective effects of the rosemary phenolic diterpene carnosic acid (CA) has attracted increasing attention. We explored the mechanism by which CA modulates the neurotoxic effects of 6-hydroxydopamine (6-OHDA) in SH-SY5Y cells. Cells were pretreated with CA for 12 h followed by treatment with 100 μM 6-OHDA for 12 or 24 h. Cell viability determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolim bromide (MTT) assay indicated that 0.1 to 1 μM CA dose-dependently attenuated the cell death induced by 6-OHDA, whereas the effect of 3-5 μM CA was weaker. CA at 1 μM suppressed the 6-OHDA-induced nuclear condensation, reactive oxygen species generation, and cleavage of caspase 3 and PARP. Immunoblots showed that the phosphorylation of c-Jun NH(2)-terminal kinase (JNK) and p38 by 6-OHDA was reduced in the presence of CA. Incubation of cells with CA resulted in significant increases in the total glutathione (GSH) level and the protein expression of the γ-glutamylcysteine ligase catalytic subunit and modifier subunit. L-Buthionine-sulfoximine, an inhibitor of GSH synthesis, attenuated the effect of CA on cell death and apoptosis. Treatment with CA also led to an increase in nuclear factor erythroid-2 related factor 2 (Nrf2) activation, antioxidant response element (ARE)-luciferase reporter activity, and DNA binding to the ARE. Silencing of Nrf2 expression alleviated the reversal of p38 and JNK1/2 activation by CA. These results suggest that the attenuation of 6-OHDA-induced apoptosis by CA is associated with the Nrf2-driven synthesis of GSH, which in turn down-regulates the JNK and p38 signaling pathways. The CA compound may be a promising candidate for neuroprotection in Parkinson's disease. | en_US |
dc.language.iso | en_US | en_US |
dc.relation.ispartof | Chemical research in toxicology | en_US |
dc.title | Carnosic acid prevents 6-hydroxydopamine-induced cell death in SH-SY5Y cells via mediation of glutathione synthesis | en_US |
dc.type | journal article | en_US |
dc.identifier.doi | 10.1021/tx300171u | - |
dc.identifier.pmid | 22894569 | - |
dc.identifier.isi | WOS:000308777100011 | - |
dc.relation.journalvolume | 25 | en_US |
dc.relation.journalissue | 9 | en_US |
dc.relation.pages | 1893-1901 | en_US |
dc.identifier.eissn | 1520-5010 | - |
item.cerifentitytype | Publications | - |
item.grantfulltext | none | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.fulltext | no fulltext | - |
item.languageiso639-1 | en_US | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Department of Food Science | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
顯示於: | 食品科學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。