http://scholars.ntou.edu.tw/handle/123456789/24638
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Melano, Ivonne | en_US |
dc.contributor.author | Chen, Hui-Jye | en_US |
dc.contributor.author | Ngwira, Loveness | en_US |
dc.contributor.author | Hsu, Pang-Hung | en_US |
dc.contributor.author | Kuo, Li-Lan | en_US |
dc.contributor.author | Noriega, Lloyd | en_US |
dc.contributor.author | Su, Wen-Chi | en_US |
dc.date.accessioned | 2024-03-05T07:53:28Z | - |
dc.date.available | 2024-03-05T07:53:28Z | - |
dc.date.issued | 2024/1/1 | - |
dc.identifier.issn | 1661-6596 | - |
dc.identifier.uri | http://scholars.ntou.edu.tw/handle/123456789/24638 | - |
dc.description.abstract | How ACE2 functions as the major host receptor of SARS-CoV-2 despite having low expression in the lungs is still unknown. To facilitate the development of therapeutic strategies against coronaviruses, gaining a deeper comprehension of the molecular mechanism of SARS-CoV-2 infection is imperative. In our previous study, we identified several potential host factors of SARS-CoV-2 using an shRNA arrayed screen, one of which was Wnt3a. Here, we validated the significance of Wnt3a, a potent activator of the Wnt/beta-catenin signaling pathway, for SARS-CoV-2 entry into cells by evaluating the effects of its knockdown and overexpression on SARS-CoV-2 pseudotyped virus entry. Further analysis revealed that SARS-CoV-2 pseudotyped virus infection activates the canonical Wnt/beta-catenin signaling pathway, which we found could subsequently stimulate ACE2 transcription. Collectively, our study identified Wnt3a as an important host factor that facilitates ACE2-mediated virus infection. Insight into the virus entry mechanism is impactful as it will aid in developing novel therapeutic strategies against current and future coronavirus pandemics. | en_US |
dc.language.iso | English | en_US |
dc.publisher | MDPI | en_US |
dc.relation.ispartof | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | en_US |
dc.subject | SARS-CoV-2 | en_US |
dc.subject | Wnt3a | en_US |
dc.subject | beta-catenin | en_US |
dc.subject | canonical pathway | en_US |
dc.subject | host factor | en_US |
dc.subject | virus entry | en_US |
dc.subject | ACE2 | en_US |
dc.title | Wnt3a Facilitates SARS-CoV-2 Pseudovirus Entry into Cells | en_US |
dc.type | journal article | en_US |
dc.identifier.doi | 10.3390/ijms25010217 | - |
dc.identifier.isi | WOS:001141498400001 | - |
dc.relation.journalvolume | 25 | en_US |
dc.relation.journalissue | 1 | en_US |
dc.identifier.eissn | 1422-0067 | - |
item.openairetype | journal article | - |
item.languageiso639-1 | English | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Department of Bioscience and Biotechnology | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | Bachelor Degree Program in Marine Biotechnology | - |
crisitem.author.orcid | 0000-0001-6873-6434 | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | College of Life Sciences | - |
Appears in Collections: | 生命科學暨生物科技學系 |
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