http://scholars.ntou.edu.tw/handle/123456789/3718
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Lai, Pei-Xin | en_US |
dc.contributor.author | Chen, Chung-Wein | en_US |
dc.contributor.author | Wei, Shih-Chun | en_US |
dc.contributor.author | Lin, Tzu-Yu | en_US |
dc.contributor.author | Jian, Hong-Jyuan | en_US |
dc.contributor.author | Lai, Irving Po-Jung | en_US |
dc.contributor.author | Mao, Ju-Yi | en_US |
dc.contributor.author | Hsu, Pang-Hung | en_US |
dc.contributor.author | Lin, Han-Jia | en_US |
dc.contributor.author | Tzou, Wen-Shyong | en_US |
dc.contributor.author | Chen, Shiow-Yi | en_US |
dc.contributor.author | Harroun, Scott G. | en_US |
dc.contributor.author | Lai, Jui-Yang | en_US |
dc.contributor.author | Huang, Chih-Ching | en_US |
dc.date.accessioned | 2020-11-18T09:30:04Z | - |
dc.date.available | 2020-11-18T09:30:04Z | - |
dc.date.issued | 2016-12 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | http://scholars.ntou.edu.tw/handle/123456789/3718 | - |
dc.description.abstract | Angiogenesis is the process of formation of new blood vessels, which is essential to human biology, and also plays a crucial role in several pathologies such as tumor growth and metastasis, exudative age related macular degeneration, and ischemia. Vascular endothelial growth factor (VEGF), in particular, VEGF-A(165) is the most important pro-angiogenic factor for angiogenesis. Thus, blocking the interaction between VEGFs and their receptors is considered an effective anti-angiogenic strategy. We demonstrate for that first time that bovine serum albumin-capped graphene oxide (BSA-GO) exhibits high stability in physiological saline solution and possesses ultrastrong binding affinity towards VEGF-A(165) [dissociation constant (K-d) similar to 3 x 10(-12) M], which is at least five orders of magnitude stronger than that of high abundant plasma proteins such as human serum albumin, fibrinogen, transferrin, and immunoglobulin G. Due to the surprising binding specificity of BSA GO for VEGF-A(165) in complex plasma fluid, we have also studied the anti-angiogenic effects in vitro and in vivo. Results show that BSA GO not only effectively inhibits the proliferation, migration and tube formation of human umbilical vein endothelial cells, but also strongly disturbs the physiological process of angiogenesis in chick chorioallantoic membrane and blocks VEGF-A(165)-induced blood vessel formation in rabbit corneal neovascularization. Our findings indicate that GO nanomaterials can potentially act as therapeutic anti-angiogenic agents via ultrastrong VEGF adsorption and its activity suppression. (C) 2016 Elsevier Ltd. All rights reserved. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | ELSEVIER SCI LTD | en_US |
dc.relation.ispartof | BIOMATERIALS | en_US |
dc.subject | ENDOTHELIAL GROWTH-FACTOR | en_US |
dc.subject | CORNEAL NEOVASCULARIZATION | en_US |
dc.subject | TUMOR ANGIOGENESIS | en_US |
dc.subject | CRITICAL REGULATOR | en_US |
dc.subject | CELL MIGRATION | en_US |
dc.subject | IN-VITRO | en_US |
dc.subject | NANOPARTICLES | en_US |
dc.subject | DELIVERY | en_US |
dc.subject | BINDING | en_US |
dc.subject | SERUM | en_US |
dc.title | Ultrastrong trapping of VEGF by graphene oxide: Anti-angiogenesis application | en_US |
dc.type | journal article | en_US |
dc.identifier.doi | 10.1016/j.biomaterials.2016.09.005 | - |
dc.identifier.isi | WOS:000386407500002 | - |
dc.identifier.url | <Go to ISI>://WOS:000386407500002 | - |
dc.relation.journalvolume | 109 | en_US |
dc.relation.pages | 12-22 | en_US |
item.languageiso639-1 | en_US | - |
item.cerifentitytype | Publications | - |
item.openairetype | journal article | - |
item.grantfulltext | none | - |
item.fulltext | no fulltext | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Department of Bioscience and Biotechnology | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | Bachelor Degree Program in Marine Biotechnology | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Department of Bioscience and Biotechnology | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | Bachelor Degree Program in Marine Biotechnology | - |
crisitem.author.dept | Center of Excellence for the Oceans | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Department of Bioscience and Biotechnology | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Department of Bioscience and Biotechnology | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Department of Bioscience and Biotechnology | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | Center of Excellence for the Oceans | - |
crisitem.author.orcid | 0000-0001-6873-6434 | - |
crisitem.author.orcid | 0000-0002-4929-6573 | - |
crisitem.author.orcid | 0000-0002-6726-1390 | - |
crisitem.author.orcid | 0000-0002-0363-1129 | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
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