http://scholars.ntou.edu.tw/handle/123456789/3817
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Zih-Jhan Huang | en_US |
dc.contributor.author | Shih-Ting Kang | en_US |
dc.contributor.author | Jiann-Horng Leu | en_US |
dc.contributor.author | Li-Li Chen | en_US |
dc.date.accessioned | 2020-11-18T09:58:33Z | - |
dc.date.available | 2020-11-18T09:58:33Z | - |
dc.date.issued | 2013-09 | - |
dc.identifier.issn | 1050-4648 | - |
dc.identifier.uri | http://scholars.ntou.edu.tw/handle/123456789/3817 | - |
dc.description.abstract | The white spot syndrome virus (WSSV) has had a serious economic impact on the global shrimp aquaculture industry in the past two decades. Although research has clarified a lot about its genome and structure, the mechanism of how WSSV enters a cell is still unclear. In this study to determine this mechanism, primary cultured hemocytes were used as an experimental model to observe the process of WSSV entry because the stable shrimp cell lines for WSSV infection are lacking. After labeling virions and endosomes with fluorescent dyes followed by observation with a confocal microscope, the results show that the WSSV colocalizes with early endosomes. Hemocytes are further treated with different endocytic inhibitors, methyl-β-cyclodextrin (MβCD) and chlorpromazine (CPZ). WSSV still can be detected in the hemocytes treated with CPZ, but not in the hemocytes treated with MβCD. Thus, we conclude that WSSV adopts the caveolae-mediated endocytosis to enter the shrimp cell. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Elsevier | en_US |
dc.relation.ispartof | Fish & Shellfish Immunology | en_US |
dc.subject | white spot syndrome virus (wssv) | en_US |
dc.subject | Primary cultured hemocytes | en_US |
dc.subject | Endosome | en_US |
dc.subject | Caveolae-mediated endocytosis | en_US |
dc.title | Endocytic pathway is indicated for white spot syndrome virus (WSSV) entry in shrimp | en_US |
dc.type | journal article | en_US |
dc.identifier.doi | <Go to ISI>://WOS:000324511700010 | - |
dc.identifier.doi | <Go to ISI>://WOS:000324511700010 | - |
dc.identifier.doi | <Go to ISI>://WOS:000324511700010 | - |
dc.identifier.doi | <Go to ISI>://WOS:000324511700010 | - |
dc.identifier.doi | 10.1016/j.fsi.2013.05.028 | - |
dc.identifier.doi | <Go to ISI>://WOS:000324511700010 | - |
dc.identifier.doi | <Go to ISI>://WOS:000324511700010 | - |
dc.identifier.url | <Go to ISI>://WOS:000324511700010 | - |
dc.relation.journalvolume | 35 | en_US |
dc.relation.journalissue | 3 | en_US |
dc.relation.pages | 707-715 | en_US |
item.grantfulltext | none | - |
item.languageiso639-1 | en | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.fulltext | no fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | journal article | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Institute of Marine Biology | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | Bachelor Degree Program in Marine Biotechnology | - |
crisitem.author.dept | Doctoral Degree Program in Marine Biotechnology | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Institute of Marine Biology | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | Bachelor Degree Program in Marine Biotechnology | - |
crisitem.author.dept | Doctoral Degree Program in Marine Biotechnology | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Institute of Marine Biology | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | Bachelor Degree Program in Marine Biotechnology | - |
crisitem.author.dept | Doctoral Degree Program in Marine Biotechnology | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Institute of Marine Biology | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | Doctoral Degree Program in Marine Biotechnology | - |
crisitem.author.orcid | 0000-0002-9593-8004 | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | College of Life Sciences | - |
Appears in Collections: | 海洋生物研究所 |
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