http://scholars.ntou.edu.tw/handle/123456789/3819
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Zi-Ning Kao | en_US |
dc.contributor.author | Chun-Hung Liu | en_US |
dc.contributor.author | Wang-Jing Liu | en_US |
dc.contributor.author | Ramya Kumar | en_US |
dc.contributor.author | Jiann-Horng Leu | en_US |
dc.contributor.author | Han-Ching Wang | en_US |
dc.date.accessioned | 2020-11-18T09:58:34Z | - |
dc.date.available | 2020-11-18T09:58:34Z | - |
dc.date.issued | 2020-11 | - |
dc.identifier.issn | 1050-4648 | - |
dc.identifier.uri | http://scholars.ntou.edu.tw/handle/123456789/3819 | - |
dc.description.abstract | Since the mechanisms by which cellular factors modulate replication of the shrimp viral pathogen white spot syndrome virus (WSSV) are still largely unknown, here we consider the sirtuins, a family of NAD+-dependent protein deacetylases that are known to function as regulatory factors that activate or suppress viral transcription and replication in mammals. In particular, we focus on SIRT1 by isolating and characterizing LvSIRT1 from white shrimp (Litopenaeus vannamei) and investigating its involvement in WSSV infection. DsRNA-mediated gene silencing led to the expression of WSSV genes and the replication of genomic DNAs being significantly decreased in LvSIRT1-silenced shrimp. The deacetylase activity of LvSIRT1 was significantly induced at the early stage (2 hpi) and the genome replication stage (12 hpi) of WSSV replication, but decreased at the late stage of WSSV replication (24 hpi). Treatment with the SIRT1 activator resveratrol further suggested that LvSIRT1 activation increased the expression of several WSSV genes (IE1, VP28 and ICP11). Lastly, we used transfection and dual luciferase assays in Sf9 insect cells to show that while the overexpression of LvSIRT1 facilitates the promoter activity of WSSV IE1, this enhancement of WSSV IE1 expression is achieved by a transactivation pathway that is NF-κB-independent. | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER | en_US |
dc.relation.ispartof | Fish & Shellfish Immunology | en_US |
dc.subject | White spot syndrome virus (WSSV) | en_US |
dc.subject | Litopenaeus vannamei | en_US |
dc.subject | LvSIRT1 | en_US |
dc.subject | WSSV IE1 | en_US |
dc.title | Shrimp SIRT1 activates of the WSSV IE1 promoter independently of the NF-kappa B binding site | en_US |
dc.type | journal article | en_US |
dc.identifier.doi | <Go to ISI>://WOS:000579494400094 | - |
dc.identifier.doi | <Go to ISI>://WOS:000579494400094 | - |
dc.identifier.doi | <Go to ISI>://WOS:000579494400094 | - |
dc.identifier.doi | 10.1016/j.fsi.2020.08.034 | - |
dc.identifier.doi | <Go to ISI>://WOS:000579494400094 | - |
dc.identifier.isi | WOS:000579494400094 | - |
dc.identifier.url | <Go to ISI>://WOS:000579494400094 | |
dc.relation.journalvolume | 106 | en_US |
dc.relation.pages | 910-919 | en_US |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.fulltext | no fulltext | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Institute of Marine Biology | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | Bachelor Degree Program in Marine Biotechnology | - |
crisitem.author.dept | Doctoral Degree Program in Marine Biotechnology | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | College of Life Sciences | - |
Appears in Collections: | 海洋生物研究所 |
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