http://scholars.ntou.edu.tw/handle/123456789/3822
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jiann-Horng Leu | - |
dc.contributor.author | Li-Li Chen | - |
dc.contributor.author | Ying-Ru Lin | - |
dc.contributor.author | Guang-Hsiung Kou | - |
dc.contributor.author | Chu-Fang Lo | - |
dc.date.accessioned | 2020-11-18T09:58:35Z | - |
dc.date.available | 2020-11-18T09:58:35Z | - |
dc.date.issued | 2010-10 | - |
dc.identifier.issn | 0145-305X | - |
dc.identifier.uri | http://scholars.ntou.edu.tw/handle/123456789/3822 | - |
dc.description.abstract | AAP-1 (WSSV449), an anti-apoptosis protein encoded by white spot syndrome virus (WSSV), blocked apoptosis in insect cells (SF9) induced by Penaeus monodon effector caspase (Pm caspase). Here, to characterize in detail the anti-Pm caspase activity of AAP-1, both proteins were expressed and purified from Escherichia coli and their interactions were assayed in vitro. We found that although AAP-1 could inhibit Pm caspase activity, the inhibition was not as efficient as that of baculovirus anti-apoptosis protein P35. We further confirmed the binding and cleavage of AAP-1 by Pm caspase, and detected three AAP-1 cleavage products. Mutational analysis and protein N-terminal sequencing revealed that whereas both Asp233 and Asp272 residues of AAP-1 are involved in binding and cleavage by Pm caspase, only the Asp272 is involved in Pm caspase inhibition. Asp233, on the other hand, negatively regulates AAP-1's anti-Pm caspase activity. Lastly, AAP-1 homotypically interacts with each other both in vitro and in insect cells. | - |
dc.language.iso | en | - |
dc.publisher | Elsevier | - |
dc.relation.ispartof | Developmental and Comparative Immunology | - |
dc.subject | wssv | - |
dc.subject | AAP-1 | - |
dc.subject | WSSV449 | - |
dc.subject | ORF390 | - |
dc.subject | Anti-apoptosis | - |
dc.subject | Pm caspase | - |
dc.title | Molecular mechanism of the interactions between white spot syndrome virus anti-apoptosis protein AAP-1 (WSSV449) and shrimp effector caspase | - |
dc.type | journal article | - |
dc.identifier.doi | 10.1016/j.dci.2010.05.010 | - |
dc.identifier.doi | <Go to ISI>://WOS:000281174400005 | - |
dc.identifier.url | <Go to ISI>://WOS:000281174400005 | - |
dc.relation.journalvolume | 34 | - |
dc.relation.journalissue | 10 | - |
dc.relation.pages | 1068-1074 | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.fulltext | no fulltext | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Institute of Marine Biology | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | Bachelor Degree Program in Marine Biotechnology | - |
crisitem.author.dept | Doctoral Degree Program in Marine Biotechnology | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Institute of Marine Biology | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | Doctoral Degree Program in Marine Biotechnology | - |
crisitem.author.orcid | 0000-0002-9593-8004 | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | College of Life Sciences | - |
Appears in Collections: | 海洋生物研究所 |
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