http://scholars.ntou.edu.tw/handle/123456789/3828
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jiann-Horng Leu | en_US |
dc.contributor.author | Kuan-Fu Liu | en_US |
dc.contributor.author | Kuan-Yu Chen | en_US |
dc.contributor.author | Shu-Hwa Chen | en_US |
dc.contributor.author | Yu-Bin Wang | en_US |
dc.contributor.author | Chung-Yen Lin | en_US |
dc.contributor.author | Chu-Fang Lo | en_US |
dc.date.accessioned | 2020-11-18T09:58:36Z | - |
dc.date.available | 2020-11-18T09:58:36Z | - |
dc.date.issued | 2015-04 | - |
dc.identifier.issn | 0145-305X | - |
dc.identifier.uri | http://scholars.ntou.edu.tw/handle/123456789/3828 | - |
dc.description.abstract | By microarray screening, we identified a white spot syndrome virus (WSSV)-strongly induced novel gene in gills of Penaeus monodon. The gene, PmERP15, encodes a putative transmembrane protein of 15 kDa, which only showed some degree of similarity (54–59%) to several unknown insect proteins, but had no hits to shrimp proteins. RT-PCR showed that PmERP15 was highly expressed in the hemocytes, heart and lymphoid organs, and that WSSV-induced strong expression of PmERP15 was evident in all tissues examined. Western blot analysis likewise showed that WSSV strongly up-regulated PmERP15 protein levels. In WSSV-infected hemocytes, immunofluorescence staining showed that PmERP15 protein was colocalized with an ER enzyme, protein disulfide isomerase, and in Sf9 insect cells, PmERP15-EGFP fusion protein colocalized with ER -Tracker™ Red dye as well. GRP78, an ER stress marker, was found to be up-regulated in WSSV-infected P. monodon, and both PmERP15 and GRP78 were up-regulated in shrimp injected with ER stress inducers tunicamycin and dithiothreitol. Silencing experiments showed that although PmERP15 dsRNA-injected shrimp succumbed to WSSV infection more rapidly, the WSSV copy number had no significant changes. These results suggest that PmERP15 is an ER stress-induced, ER resident protein, and its induction in WSSV-infected shrimp is caused by the ER stress triggered by WSSV infection. Furthermore, although PmERP15 has no role in WSSV multiplication, its presence is essential for the survival of WSSV-infected shrimp. | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVLER | en_US |
dc.relation.ispartof | Developmental and Comparative Immunology | en_US |
dc.subject | White spot syndrome virus | en_US |
dc.subject | Penaeus monodon | en_US |
dc.subject | ER stress | en_US |
dc.subject | Unfolded protein response | en_US |
dc.title | The novel white spot syndrome virus-induced gene, PmERP15, encodes an ER stress-responsive protein in black tiger shrimp, Penaeus monodon | en_US |
dc.type | journal article | en_US |
dc.identifier.doi | <Go to ISI>://WOS:000349880500005 | - |
dc.identifier.doi | <Go to ISI>://WOS:000349880500005 | - |
dc.identifier.doi | 10.1016/j.dci.2014.12.001 | - |
dc.identifier.doi | <Go to ISI>://WOS:000349880500005 | - |
dc.identifier.doi | <Go to ISI>://WOS:000349880500005 | - |
dc.identifier.isi | WOS:000349880500005 | - |
dc.identifier.url | <Go to ISI>://WOS:000349880500005 | |
dc.relation.journalvolume | 49 | en_US |
dc.relation.journalissue | 2 | en_US |
dc.relation.pages | 239-248 | en_US |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.fulltext | no fulltext | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Institute of Marine Biology | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | Bachelor Degree Program in Marine Biotechnology | - |
crisitem.author.dept | Doctoral Degree Program in Marine Biotechnology | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | College of Life Sciences | - |
Appears in Collections: | 海洋生物研究所 |
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