http://scholars.ntou.edu.tw/handle/123456789/5476
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Pai, Wan-Yu | en_US |
dc.contributor.author | Lo, Wan-Yu | en_US |
dc.contributor.author | Hsu, Todd | en_US |
dc.contributor.author | Peng, Ching-Tien | en_US |
dc.contributor.author | Wang, Huang-Joe | en_US |
dc.date.accessioned | 2020-11-19T10:20:12Z | - |
dc.date.available | 2020-11-19T10:20:12Z | - |
dc.date.issued | 2017-12-8 | - |
dc.identifier.issn | 1664-042X | - |
dc.identifier.uri | http://scholars.ntou.edu.tw/handle/123456789/5476 | - |
dc.description.abstract | Background and Aims: The angiotensin-(1-7)/angiotensin-converting enzyme 2/Mas receptor axis counter-regulates the detrimental effects of angiotensin II. Beneficial effects of angiotensin-(1-7), including anti-inflammation, oxidative stress reduction, and anti-thrombosis, have been reported. Previous studies documented that ramipril decreased thrombin generation in human hypertension and that the anti-thrombotic effects of captopril and losartan were angiotensin-(1-7)-dependent, suggesting an interaction between thrombin and angiotensin-(1-7). However, it is not clear whether angiotensin-(1-7) can alleviate the endothelial phenotypic changes induced by thrombin. We have previously documented cytoskeleton remodeling, cell adhesion, and cell migration as dominant altered phenotypes in thrombin-stimulated human aortic endothelial cells (HAECs). In this study, we investigated whether angiotensin-(1-7) can modulate thrombin-induced phenotypic changes. Furthermore, we investigated whether NAPDH oxidase 5 (Nox5)-produced reactive oxygen species (ROS) play a significant role in angiotensin-(1-7)-mediated phenotypic changes. Methods: HAECs were pretreated with 100 nM angiotensin-(1-7) for 1 h, followed by stimulation with 2 units/mL thrombin for different times. Immunofluorescent assay, monocyte adhesion assay, wound-healing assay, ROS assay, real-time PCR, Western blotting, and Nox5 siRNA transfection were conducted. HAECs were pretreated with the ROS scavenger N-acetylcysteine (NAG) to determine whether thrombin-induced phenotypic changes depended on ROS production. Results: Angiotensin-(1-7) prevented thrombin-induced actin cytoskeleton derangements, monocyte adhesion, and migratory impairment. Nox5 siRNA transfection confirmed that thrombin-induced Nox5 expression stimulated ROS production and increased HO-1/NQO-1/ICAM-1/VCAM-1 gene expression, all of which were decreased by angiotensin-(1-7). Phenotypic changes induced by thrombin were prevented by NAG pretreatment. Conclusion: Angiotensin-(1-7) prevents actin cytoskeleton derangement, monocyte adhesion, and migration impairment induced by thrombin via downregulation of ROS production. In addition, thrombin-induced Nox5 expression is involved in the production of ROS, and angiotensin-(1-7) decreases ROS through its inhibitory effect on Nox5 expression. | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | FRONTIERS MEDIA SA | en_US |
dc.relation.ispartof | FRONT PHYSIOL | en_US |
dc.subject | DIABETIC HYPERTENSIVE-RATS | en_US |
dc.subject | REDUCING OXIDATIVE STRESS | en_US |
dc.subject | INDUCED DYSFUNCTION | en_US |
dc.subject | DEPENDENT PATHWAYS | en_US |
dc.subject | CELL MIGRATION | en_US |
dc.subject | RECEPTOR MAS | en_US |
dc.subject | INFLAMMATION | en_US |
dc.subject | ACTIVATION | en_US |
dc.subject | DISEASE | en_US |
dc.subject | ISCHEMIA | en_US |
dc.title | Angiotensin-(1-7) Inhibits Thrombin-Induced Endothelial Phenotypic Changes and Reactive Oxygen Species Production via NADPH Oxidase 5 Downregulation | en_US |
dc.type | journal article | en_US |
dc.identifier.doi | 10.3389/fphys.2017.00994 | - |
dc.identifier.isi | WOS:000417478000001 | - |
dc.identifier.url | <Go to ISI>://WOS:000417478000001 | |
dc.relation.journalvolume | 8 | en_US |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en_US | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Department of Bioscience and Biotechnology | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | Bachelor Degree Program in Marine Biotechnology | - |
crisitem.author.dept | Doctoral Degree Program in Marine Biotechnology | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | College of Life Sciences | - |
顯示於: | 生命科學暨生物科技學系 03 GOOD HEALTH AND WELL-BEING |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。