http://scholars.ntou.edu.tw/handle/123456789/6081
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.author | Po-Tsang Lee | en_US |
dc.contributor.author | Chiu-Ming Wen | en_US |
dc.contributor.author | Fan-Hua Nan | en_US |
dc.contributor.author | Han-Yang Yeh | en_US |
dc.contributor.author | Meng-Chou Lee | en_US |
dc.date.accessioned | 2020-11-20T00:40:28Z | - |
dc.date.available | 2020-11-20T00:40:28Z | - |
dc.date.issued | 2020-08 | - |
dc.identifier.issn | 1050-4648 | - |
dc.identifier.uri | http://scholars.ntou.edu.tw/handle/123456789/6081 | - |
dc.description.abstract | In this study, water extracts of the red seaweed Sarcodia suiae were obtained using solid-liquid extraction (SLE) or pressurized liquid extraction (PLE) methods. The extracts were used to investigate immunostimulatory activity by measuring the phagocytic activity of Nile tilapia (Oreochromis niloticus) hepatic and splenic macrophages and the tilapia head kidney (THK) cell line, and modulation of immune-related genes in primary head kidney (HK) cells and THK cells. At 10 μg/ml, both extracts promoted the proliferation of hepatic and splenic macrophages. Expression levels of proinflammatory cytokines (IL-1β and IL-8), antimicrobial peptides (TP2 and TP4), and pattern recognition receptors (TLR5) were elevated in SLE extracts-treated primary HK leukocytes. Similarly, IL-1β, IL-8, and TNFα expression was also induced by SLE extract in THK cells. Phagocytic activity in primary HK cells and THK cells was induced by SLE extract 12 h and 24 h post-stimulation, while PLE extract only induced phagocytic activity in THK cells at early time points. SLE extract (100 μg/g body weight) increased the expression of IL-1β, IL-8, TNFα, TP2, TP4, TLR2 and TLR5 in the spleen and immunoprotective efficiency against Streptococcus agalactiae infection. Taken together, these results show that S. suiae can differentially stimulate the immune response of tilapia in vitro and in vivo and could potentially be used as an immunomodulator in tilapia culture. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Elsevier | en_US |
dc.relation.ispartof | Fish & Shellfish Immunology | en_US |
dc.subject | Nile tilapia | en_US |
dc.subject | Immunostimulants | en_US |
dc.subject | Sarcodia | en_US |
dc.subject | Phagocytosis | en_US |
dc.subject | Innate immunity | en_US |
dc.subject | Gene expression | en_US |
dc.title | Immunostimulatory effects of Sarcodia suiae water extracts on Nile tilapia Oreochromis niloticus and its resistance against Streptococcus agalactiae | en_US |
dc.type | journal article | en_US |
dc.identifier.doi | 10.1016/j.fsi.2020.05.017 | - |
dc.identifier.isi | 000540375500019 | - |
dc.relation.journalvolume | 103 | en_US |
dc.relation.pages | 159-168 | en_US |
item.grantfulltext | none | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.fulltext | no fulltext | - |
item.cerifentitytype | Publications | - |
item.languageiso639-1 | en | - |
item.openairetype | journal article | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Department of Aquaculture | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Department of Aquaculture | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Department of Aquaculture | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.dept | Center of Excellence for Ocean Engineering | - |
crisitem.author.dept | Bachelor Degree Program in Marine Biotechnology | - |
crisitem.author.dept | Ocean Energy and Engineering Technology | - |
crisitem.author.orcid | https://orcid.org/ 0000-0003-3657-2599 | - |
crisitem.author.orcid | 0000-0003-4133-7171 | - |
crisitem.author.orcid | 0000-0002-9646-1068 | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
crisitem.author.parentorg | Center of Excellence for Ocean Engineering | - |
顯示於: | 水產養殖學系 |
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