http://scholars.ntou.edu.tw/handle/123456789/9956
標題: | Application of LC-MS/MS in Identification of Toxin in the Causative Gastropod and Victim Samples | 作者: | Chun-Lan Lin Cheng-Hong Hsieh You-Liang Hsieh Yi-Cheng Tai Deng-Fwu Hwang |
關鍵字: | food poisoning;gastropod;TTX;Nassariidae;LC-MS/MS | 公開日期: | 1-十二月-2013 | 出版社: | National Taiwan Ocean University | 卷: | 21 | 期: | 7 | 起(迄)頁: | 52-57 | 來源出版物: | Journal of Marine Science and Technology | 摘要: | Food paralytic poisoning incident was detected in the gastropod Nassariidae Niotha clathrata and Zeuxis scalaris occurred in December 2012 in Kaohsiung, Taiwan. The toxin was purified from each remain of cooked gastropod and victim tissues by ultrafiltration using a YM-3 membrane, followed by C18 cartridge column purification. Based on analyses by LC-MS/MS, the causative agent of gastropod food poisoning was identified as tetrodotoxin. The toxicity of remain of cooked sample was 245 MU/g (1MU = 0.178 μg TTX) for N. clathrata and 203 MU/g for Z. scalaris. The blood and excrements of victim was shown to contain TTX of 0.13 and 0.18 μg/g, respectively. There was positive between results from LC-MS/MS and mouse bioassay in the causative gastropod. The LC-MS/MS method is suitable for rapid screening of TTX. |
URI: | http://scholars.ntou.edu.tw/handle/123456789/9956 | ISSN: | 1023-2796 | DOI: | 10.6119/jmst-013-1223-1 |
顯示於: | 食品科學系 |
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