http://scholars.ntou.edu.tw/handle/123456789/9972
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wen-Feng Lin | en_US |
dc.contributor.author | Yao-Cheng Lyu | en_US |
dc.contributor.author | Ya-Jung Wu | en_US |
dc.contributor.author | Chi-Huan Lu | en_US |
dc.contributor.author | Deng-Fwu Hwang | en_US |
dc.date.accessioned | 2020-11-21T02:41:12Z | - |
dc.date.available | 2020-11-21T02:41:12Z | - |
dc.date.issued | 2012-06 | - |
dc.identifier.issn | 0956-7135 | - |
dc.identifier.uri | http://scholars.ntou.edu.tw/handle/123456789/9972 | - |
dc.description.abstract | A snapper (Lutjanidae fish) is a carnivorous coral reef fish that is distributed in sea areas around Taiwan. In December 2008 in southern Taiwan, a food poisoning incident occurred due to the ingestion of snapper, and the causative residue of ciguatera was investigated using a toxicity assay. To identify the species of the causative sample, six suspected species of Lutjanidae fish commonly found in Taiwan were analyzed using both sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) techniques. According to the low molecular weight region (<30.0 kD) of species-specific patterns extracted from myofibrillar and sarcoplasmic proteins, the 6 snapper species could be clearly differentiated by the SDS-PAGE method. Furthermore, a consistent 465 bp sequence of the mitochondrial cytochrome b gene from the 6 snapper species was amplified by the PCR method and was rapidly distinguished by the analysis of restriction enzymes. According to both SDS-PAGE and PCR-RFLP methods, the poisonous sample was identified as Lutjanus bohar, which is also a notorious Lutjanidae species containing ciguateric toxins. | en_US |
dc.language.iso | en | en_US |
dc.publisher | ELSEVIER | en_US |
dc.relation.ispartof | Food Control | en_US |
dc.subject | Snapper | en_US |
dc.subject | Species identification | en_US |
dc.subject | SDS-PAGE | en_US |
dc.subject | PCR-RFLP | en_US |
dc.title | Species identification of snapper: A food poisoning incident in Taiwan | en_US |
dc.type | journal article | en_US |
dc.identifier.doi | 10.1016/j.foodcont.2011.11.028 | - |
dc.relation.journalvolume | 25 | en_US |
dc.relation.journalissue | 2 | en_US |
dc.relation.pages | 511-515 | en_US |
item.cerifentitytype | Publications | - |
item.openairetype | journal article | - |
item.openairecristype | http://purl.org/coar/resource_type/c_6501 | - |
item.fulltext | no fulltext | - |
item.grantfulltext | none | - |
item.languageiso639-1 | en | - |
crisitem.author.dept | College of Life Sciences | - |
crisitem.author.dept | Department of Food Science | - |
crisitem.author.dept | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | National Taiwan Ocean University,NTOU | - |
crisitem.author.parentorg | College of Life Sciences | - |
Appears in Collections: | 食品科學系 |
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