建立快速自動化石斑病毒檢測平台(I)

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Project title

建立快速自動化石斑病毒檢測平台(I)

Code/計畫編號

NSC98-2324-B019-003-CC1

Translated Name/計畫中文名

建立快速自動化石斑病毒檢測平台(I)

Project Coordinator/計畫主持人

Ming-Wei Lu

Funding Organization/主管機關

National Science and Technology Council

Co-Investigator(s)/共同執行人

吳金洌

Department/Unit

Department of Aquaculture

Website

https://www.grb.gov.tw/search/planDetail?id=1925373

Year

2009

Start date/計畫起

01-08-2009

Expected Completion/計畫迄

31-07-2010

Bugetid/研究經費

1000千元

ResearchField/研究領域

生物技術(農)
漁業

"台灣四面環海，水產養殖發展歷程已久，台灣在石斑魚繁養殖一向在全球居於領導地位，但可惜的是在缺乏大型業者做有系統的投資，將整套生產流程標準化，故魚苗生產品質及產量一直無法固定，也對於台灣要形成亞太種苗中心有很大的阻礙，故研發一套快速，靈敏且自動化的疾病檢測平台，是一個很重要有效控制病毒性疾病的防治對策。磁量生技公司的專利MagQu 的免疫磁減量檢測系統，是利用標誌有抗體的磁珠與病毒結合後產生的磁減量現象，將微弱的磁訊號轉變為可量測的電壓訊息，具相當高的靈敏度。本計劃針對海水魚類的神經壞死症病毒(NNV)，透過磁珠轉動速度來偵測NNV病毒量，目前已經建立病毒標準曲線。接著進行敏感度試驗，結果可以偵測到 101 TCID50 /ml以下的病毒量。另外進行專一性試驗，用此平台來健側胰臟壞死症病毒及虹彩病毒等兩種病毒，所得到的讀值皆低於背景值，顯示本技術平台有可信賴的專一性。未來我們將會將這平台技術最佳化，包含反應時間的縮短及機台可攜性的研發，使得更趨近可上市產品規模，同時也會建立病毒感染及發病的的資料庫，在這個資料庫當中，我們將可以得到病毒發病的可能因子，如水溫、水質、病毒量等，綜合這些因子，將可提供數種有用的資料可供利用，如魚苗買入安全病毒量、氣候危險因子，用藥判斷等，在整合磁量生技及多家南台灣大型石斑繁養殖廠，我們希望能建立一套病毒監控平台並且這平台能夠實際應用在現場養殖當中，並落實未來產品化的可能性。" "Taiwan is surrounded with sea. The aquaculture industry was established in the very early time. The technology development in aquaculture is also in the leading position of the whole world. But lacking of big companies which are involve in the investigation in the aquaculture industry. The quality and quantity of fish larvae is not stable. That is negative for Taiwan to development as “Center of Asia fish larvae”. Research and development of a rapid, sensitive and automatic detection platform is one of the major approaches of controlling viral diseases in aquaculture. An efficient detection platform has allowed us to monitoring the spread of important pathogens and will help to enhance the economic benefit of aquaculture industry. In this project, aiming at grouper nervous necrosis virus (NNV), attempts were made to develop an ultra-high sensitive MagnetoReduction Assay (MRA) for quantitively detect the target bio-molecules (NNV viral antigens). Viral encephalopathy and retinopathy (VER), caused by betanodaviruses (piscine nodavirus), is one of the most devastating, occurring in a variety of marine fish all over the world. Firstly, basically using a solution having homogeneously dispersed magnetic nanoparticles, which are coated with anti-NNV antibody. Under external multiple ac magnetic fields, magnetic nanoparticles oscillate with the multiple ac magnetic fields via magnetic interaction. In the preliminary results, we already establish the standard curve in different NNV concentration. The results showed the sensitivity reaches 101 TCID50 /ml virus titer. We also using NNV reagent to test the IPNV and iridovirus. The reading values were below the backgroup noise. The results suggested the platform specificity is reliable. In the future, we will optimize the platform including shorten the reaction time and equipment size. We will also establish the virus infection and outbreak database. The data can be used for safety virus titter when you bought in, weather risk factor, drug usage etc. By incorporate magqu biotechnology company and hatcheries in southern Taiwan, we hope to establish a virus detection platform and the product can be used in the aquaculture industry."

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建立快速自動化石斑病毒檢測平台(I)

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