Molecular Mechanism of White Spot Syndrome Virus (WSSV) Pathogenecity: Study the Role of Glucose Transporter 1 (Glut1) in WSSV Infection

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簡歷

基本資料

Project title

Code/計畫編號

MOST103-2311-B019-001

Translated Name/計畫中文名

白點症病毒致病機轉：蝦葡萄醣通道蛋白Glut1於病毒感染過程中角色之探討

Project Coordinator/計畫主持人

Li-Li Chen

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Institute of Marine Biology

Website

https://www.grb.gov.tw/search/planDetail?id=8348181

Year

2014

Start date/計畫起

01-08-2014

Expected Completion/計畫迄

31-07-2015

Bugetid/研究經費

1220千元

ResearchField/研究領域

漁業
生物技術(農)

白點症病毒是造成蝦類嚴重死亡的病原體，為一種具封套的大型 DNA 病毒。在過去的研究中發現蝦細胞表面的葡萄醣運輸蛋白 1 可與幾丁質結合蛋白產生結合作用，我們也證實至少有 11 個病毒封套蛋白能與幾丁質結合蛋白結合，因此推測病毒封套蛋白群、幾丁質結合蛋白及葡萄醣運輸蛋白 1 可形成一感染複合體，促使病毒入侵。參考其他病毒受器的研究，以人類嗜 T 淋巴球病毒為例，此病毒受體為葡萄醣運輸蛋白 1 且形成複合體，最後被感染的血球細胞大量減少；而在 C 型肝炎病毒的例子中更發現病毒會調控葡萄醣運輸蛋白 2，使得醣份無法運送進細胞裡；人類免疫不全病毒的研究中更發現病毒的感染力與葡萄醣運輸蛋白 1 所引導的代謝途徑有關。最近在蛋白質體學上的研究也證實了白點症病毒感染後，醣類代謝途徑的數種蛋白質出現明顯變化，顯示病毒和葡萄醣運輸蛋白的結合與白點症病毒致病機轉息息相關。因此在本期的計畫冀望能解開白點症病毒調節葡萄醣運輸蛋白而導致蝦體衰敗的致病機轉，我們將先分析葡萄醣運輸蛋白 1 的功能，再分析葡萄醣運輸蛋白 1 可能參與的細胞代謝及訊息傳導途徑，以期解開白點症病毒感染蝦體之後是如何調控葡萄醣運輸蛋白導致細胞凋亡，並以此建構葡萄醣代謝體學研究。"White spot syndrome virus (WSSV), a large double-stranded enveloped DNA virus, can cause the most serious viral disease of shrimp and has a wide host range among crustaceans. The genome of WSSV is about 300 kb and can encode about 180 genes. In the previous study, a cell surface protein, glucose transporter 1 (Glut1), was verified to interact with Penaeus monodon chitin-binding protein (PmCBP or CBP), which was itself identified to interact with an envelope protein complex formed by 11 WSSV envelope proteins. Thus, we speculate that these 11 WSSV envelope proteins and 2 host proteins may form a complex, infectome, to mediate virus entry. Review in the research of the receptor for human T-cell lymphotropic virus (HTLV) has already revealed that the receptor is a Glut1 and it can form a complex with other proteins. Hepatitis C virus (HCV) can repress the expression of Glut2 in the infected cell and this causes the glucose fail to transport into cytosol. Referring in the research on human immunodeficiency virus (HIV) reveals that the Glut1-mediated metabolic pathway plays as a critical regulator of HIV-1 infection. In the late stage of WSSV infection, the amount of hemocytes in the infected shrimp was dramatically reduced and most tissues and organs showed massive multifocal necrosis. Recent studies using proteomics revealed that the proteins participated in energy production and glucose metabolism are affected after WSSV infection. It indicates that the interaction between WSSV and Glut1 may contribute to the virus pathogenesis and finally lead to shrimp death. Therefore, a project based on revealing the role of Glut1 in the pathogenesis of WSSV infection was proposed. The biological functions of the Glut1 will be analyzed firstly, and then the metabolic and signal transduction pathways which Glut1 participates in will also be characterized. We hope the study can help to understand the strategy WSSV applied to induce apoptosis by regulating Glut1, and establish the Glut1 metabolomics study. "

Keyword(s)

白點症病毒
葡萄醣運輸蛋白 1
幾丁質結合蛋白
封套蛋白
感染複合體；
致病機轉
細胞代謝及訊息傳導途徑
代謝體學
WSSV
Gluct1
PmCBP or CBP
envelope protein
infectome
pathogenesis
metabolic and signal transduction pathways
metabolomics

DSpace CRIS

Molecular Mechanism of White Spot Syndrome Virus (WSSV) Pathogenecity: Study the Role of Glucose Transporter 1 (Glut1) in WSSV Infection

基本資料

Description