Development of a Surface-Plasmon Resonance Phase Modulation Bio-Reaction Image Detection System with Quadrature Signal Algorithm

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簡歷

基本資料

Project title

Code/計畫編號

NSC98-2221-E019-027

Translated Name/計畫中文名

時進正交相移法之影像式相位調制表面電漿子共振系統之開發

Project Coordinator/計畫主持人

Shu-Sheng Lee

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Systems Engineering and Naval Architecture

Website

https://www.grb.gov.tw/search/planDetail?id=1899957

Year

2009

Start date/計畫起

01-08-2009

Expected Completion/計畫迄

01-07-2010

Bugetid/研究經費

530千元

ResearchField/研究領域

醫學工程
醫學技術

"人類文明與科技發展日新月異，生活富足、衣食無虞，因此進一步追求的是健康的身心，故近年來人類更致力於許多可促進人類生命健康，以及對於疾病控制的生命科學及醫藥科技研發。在發展生物科技的同時，更需要精良的相關檢測技術及儀器系統開發，因此應用於生物晶片技術的光學生物感測技術是全球各國看重的重要技術，特別是近十幾年來所新開發的不需特殊的螢光標示之表面電漿子共振技術(SPR, Surface Plasmon Resonance)，更是生醫光學檢測技術中最受重視的技術之一。生醫檢測過程中，待測生物分子與晶片上的生物分子結合後，以表面電漿子共振技術量測其折射率變化，進一步獲得折射率變化與待測物濃度之關係比例，而可得知待測分子於檢體中的濃度變化。本研究計畫將以表面電漿子共振技術中，最靈敏之相位調制偵測法作為整體之生醫分子反應檢測系統開發之基礎，開發影像式表面電漿子共振相位調制偵測系統。因為相移偵測架構系統靈敏度取決於系統最小相移量，以及系統自身雜訊與環境因素影響之控制，因此本計畫所採用八分之一波板和電子耦合取像裝置，配合創新之時進正交相移演算法，取代傳統干涉相移技術，將可避免前述系統最小相移量及環境因素之干擾。因為時進正交法於相位干涉過程中，可以修正因系統相位變化過大造成訊號之相關性減弱缺失之優點，另外相位計算仍為瞬時單點資訊呈現，將有助於系統即時監控量測，達成具量測陣列式大量生化樣本反應潛力的結合時進正交相移法之影像式相位調制表面電漿子共振系統。" "Human beings live in much better life than before as the developing and progress of the civilization and techniques recently. However, to live in a longer and healthier life is still everyone’s wish and many researchers are devoted themselves in the fields of life science and bio-medical technology. Therefore, the development of precision detecting platforms for biotechnology is a very important topic. For these reasons, the detecting technology based on Surface Plasmon Resonance (SPR) attracts much more attention because it has the advantage of no special designed fluoresce labeling required. SPR technology can measure the variations of the refraction index (RI) of the sample which are induced by the combination of the bio-molecules on the biochip. Further, the concentration of the bio-molecules of the sample can be obtained by calculation from the relationship between the variations of RI and the concentrations. In this project, an image SPR system adopting the phase interrogation is going to be developed because the sensitivity of phase interrogation is the best compared with the intensity, angle, and wavelength interrogation. Since the resolution and precision of the phase interrogation SPR system is decided by the value of phase shift and the environment disturbance, the quadrature signal phase decoded method has been utilized in the system. The quadrature signal phase decoded method which is realized by using the 1/8 wave plate and CCD (charge coupled device) in the system can correct the decorrelation of the interfering fringes image and reduce the requirement of image data. Therefore, the bio-detecting system developed in this research work can be implement and to be a real time image phase interrogation SPR detecting system."

Keyword(s)

表面電漿子共振
相位調制時進正交相移法斯奈爾定律中位數濾波器傅立葉轉換 Surface Plasmon Resonance Phase Interrogation Quadrature Signal PhaseDecoded Method Fresnel's Equation Noise Reduction Median Filter FourierTransformation

DSpace CRIS

Development of a Surface-Plasmon Resonance Phase Modulation Bio-Reaction Image Detection System with Quadrature Signal Algorithm

基本資料

Description