The Diversity and Biogeography of Microeukaryotes in the Subtropical Marine Coastal Ecosystem

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Project title

Code/計畫編號

MOST104-2611-M019-006

Translated Name/計畫中文名

副熱帶沿岸生態系微小真核生物多樣性與生物地理之研究

Project Coordinator/計畫主持人

Sheng-Fang Tsai

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Center of Excellence for the Oceans

Website

https://www.grb.gov.tw/search/planDetail?id=11574853

Year

2015

Start date/計畫起

10-08-2015

Expected Completion/計畫迄

31-07-2016

Bugetid/研究經費

637千元

ResearchField/研究領域

海洋科學
自然生態保育

微小動物性浮游生物是nano級(鞭毛蟲)及pico級(細菌）的主要攝食者，其中又以少毛類纖毛蟲為大宗。因此在海洋生態系裡扮演著將有機與碳能量由微生物食物網傳遞至高階傳統食物鏈的關鍵性角色。儘管先前已針對臺灣東北沿岸海域之少毛類纖毛蟲進行種類鑑定之研究，但是少毛類纖毛蟲的組成及種類、數量周年變化並不清楚。因此本計晝希望利用蛋白銀染色法及無酸Lugol’s固定法進行種類的鑑定及計數，同時以分子生物技術方法，獲得各個種類的18S rDNA序列，以進行臺灣東北沿岸海域少毛類纖毛蟲之組成與生態之研究。研究主要分為三個部分：1)藉蛋白銀染色法鑑定少毛類纖毛蟲種類；2)以18S rDNA序列分析探討少毛類纖毛蟲之生物多樣性；及3)利用無酸Lugol’s 固定之樣本計數少毛類纖毛蟲周年數量變化。以上研究有助於了解不同種類之少毛類纖毛蟲在同一副熱帶沿岸生態系海域之數量變化，進一步作為後續針對各優勢種於微生物食物網中與環境的關係及各自扮演的角色。"Microeukaryotes, mostly composed by oligotrich ciliates, are the major grazer on nanoplankton and picoplankton, and play an important role on the trophic link within microbial food web. Although previous studies have investigated the identification of oligotrichs ciliates in the northeastern coastal Taiwan, it is rarely known about the diversity of oligotrich ciliates and their annual species composition. Therefore, the goal of this project is to apply molecular techniques to elucidate the composition of oligotrich ciliates in three directions, including 1) the identification of the oligotrich ciliates by protargol staining methods; 2) the diversity of the species by 18S rDNA sequences analysis; and 3) the dial variation of species and its abundance by non-acid Lugol’s fixation. This study will strengthen our understanding of the functional role of different oligotrich ciliates in the microbial food web in a subtropical coastal ecosystem. Furthermore, the impact of global warming and acidification on the oligotrich ciliates could be monitored by observing the rapid change in their species composition, which further affecting the structure of the microbial food web."

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The Diversity and Biogeography of Microeukaryotes in the Subtropical Marine Coastal Ecosystem

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