Lab_On_A_Chip for Dna Hybridization Assays

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Project title

Code/計畫編號

NSC99-2221-E019-014

Translated Name/計畫中文名

應用實驗室晶片於DNA雜合之檢測

Project Coordinator/計畫主持人

Pei-Tai Chen

Funding Organization/主管機關

National Science and Technology Council

Co-Investigator(s)/共同執行人

苑舉民
陳昭德

Department/Unit

Department of Systems Engineering and Naval Architecture

Website

https://www.grb.gov.tw/search/planDetail?id=2116228

Year

2010

Start date/計畫起

01-08-2010

Expected Completion/計畫迄

01-07-2011

Bugetid/研究經費

630千元

ResearchField/研究領域

生物技術(醫)
其他（醫）

"本計劃的目的為發展去氧核糖核酸(DNA)雜合檢測之實驗室晶片，此晶片包含細菌之裂解(cell lysis)，DNA 之淬取(extraction)及濃縮，以及DNA 之雜合反應。其中檢測之探針(Probe)帶有螢光，可以檢測螢光來決定目標DNA 與探針是否互補。首先含有細菌之夜體通過微流道結構之薄膜纖維，此纖維孔徑大小足以阻擋細菌，如此濃縮之細菌以解離沖刷，即可把細胞膜破裂，而使其中之DNA、蛋白質等釋出於水溶液中。當此水溶液流經矽珠時，在高濃度鹽條件下，DNA 得以吸附在矽珠上，而可以從液體中把DNA 分離出來，更進一步的以低濃度鹽沖刷則可以將DNA 從矽珠上淬取至水溶液而得到純化且濃縮之DNA，這些DNA 通過先前發展成功的DNA 雜合晶片，即檢測出是否與探針雜合反應，而完成一個實驗室晶片檢測過程。" "The proposed project develops a lab-on-chip for DNA hybridization assays, including cell concentration, cell lyses, DNA extraction, and final DNA hybridization with a specific probe. The chip includes a microdevice able to concentrate bacteria from sample solutions by a membrane of which DNA, proteins and other derbies are released from the cells. The DNA, in turns, are attracted to silica beads which the purified DNA are obtained. These DNA are hybridized with a specific probe and detected fluorescently. The microfluidic chip performs the hybridization assay, including cell lyses, DNA extraction and hybridization."

Keyword(s)

愛德華氏菌
微流道晶片
微影製程
專一性探針ET996

DSpace CRIS

Lab_On_A_Chip for Dna Hybridization Assays

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