Research and Development of an Innovative Biochip Platform for Automatically, Rapidly Detecting Important Viral Pathogens in Maricultured Fishes-Nervous Necrosis Virus Detection Platform

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基本資料

Project title

Code/計畫編號

97農科-1.1.2-漁-F1(1)

Translated Name/計畫中文名

海水養殖魚類重要病原之自動化快速檢測晶片技術研發-神經壞死症病毒檢測平台

Project Coordinator/計畫主持人

Hsin-Yiu Chou

Funding Organization/主管機關

Council of Agriculture,Executive Yuan

Co-Investigator(s)/共同執行人

洪健睿
吳金洌

Department/Unit

Department of Aquaculture

Website

https://www.grb.gov.tw/search/planDetail?id=1705833

Year

2008

Start date/計畫起

01-02-2008

Expected Completion/計畫迄

31-12-2008

Bugetid/研究經費

1395千元

ResearchField/研究領域

漁業

研發一套快速，靈敏且自動化的疾病檢測平台，是目前水產養殖中可以有效控制病毒性疾病的防治對策。藉此監控重要病源的散播，減少病害損失，將可提高養殖產業的經濟效益。因此本計劃擬針對海水魚類的神經壞死症病毒，建立一套適合偵測水產病毒的高敏感度磁珠感測平台。MagQu的免疫磁減量檢測系統，是利用標誌有抗體的磁珠與病毒結合後產生的磁減量現象，將微弱的磁訊號轉變為可量測的電壓訊息，所以具有相當高的靈敏度。首先我們將會進行各種外在變因的測試，包括溫度、pH值、鹽度、試劑濃度、磁場大小等，以找出其最適合的反應條件。接著在靈敏度與專一性分析後，進而成為標準作業程序，並利於以業者方便使用。同時，評估這套系統用來偵測NNV感染早、中期病毒蛋白分子之表現之可行性，另外根據以上結果，建立虹彩病毒之流式磁珠感測技術來偵測不同海水魚類之虹彩病毒感染。 Research and development of a rapid, sensitive and automatic detection platform is one of the major approaches of controlling viral diseases in aquaculture. An efficient detection platform has allowed we to monitoring the spread of important pathogens and will help to enhance the economic benefit of aquaculture industry. In this project, aiming at grouper nervous necrosis virus (NNV), attempts were made to develop an ultra-high sensitive MagnetoReduction Assay (MRA) for quantitively detect the target bio-molecules (NNV viral antigens). The system shows a noise level lower than that of the magnetic signal generated by magnetic beads by 2-3 orders of magnitude. Hence, SQUID can detect a tiny change in the ac magnetic susceptibility of reagents due to the clustering or conjugation of magnetic beads associating with aquatic virus. Firstly, we will test a variety of extrinsic factors (temperature, pH, Salinity, reagent concentration, magnetic stretch) to figure out the optimal reaction condition for NNV. Basing on the results of sensitivity and specificity assay, standard operation procedure (SOP) for NNV detection will be completed. Subsequently, this assays will be used to determine the expression of viral proteins in early, middle stages of betanodavirus infection. Finally, the feasibility of MagnetoReduction Assay (MRA) for iridovirus detection will be assayed.

Keyword(s)

流式磁珠感測分析
神經壞死症病毒
虹彩病毒
石斑
Magnetoreduction Assays
Nervous Necrosis Virus
Iridovirus
Grouper

DSpace CRIS

Research and Development of an Innovative Biochip Platform for Automatically, Rapidly Detecting Important Viral Pathogens in Maricultured Fishes-Nervous Necrosis Virus Detection Platform

基本資料

Description