High-Throughput Rna Sequencing Identifies the Transcriptional Responses of Skeletonema to Varying Nutrient Availability during the Formation of Blooms(I)

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基本資料

Project title

Code/計畫編號

MOST109-2628-M019-001-MY4

Translated Name/計畫中文名

以高通量定序解析骨藻在藻華過程中感受環境營養鹽變化之調適機制(1/4)

Project Coordinator/計畫主持人

Lee-Kuo Kang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Bachelor Degree Program in Marine Biotechnology

Website

https://www.grb.gov.tw/search/planDetail?id=13537184

Year

2020

Start date/計畫起

01-08-2020

Expected Completion/計畫迄

31-07-2021

Bugetid/研究經費

3999千元

ResearchField/研究領域

海洋科學

在海洋生態系中，矽藻貢獻約40% 的海洋初級生產力，其中骨藻 (Skeletonema) 為沿岸河口及湧升流區經常形成藻華的優勢種，而影響它們生長控制因子經常被認為是受環境中營養鹽的供應所控制。為了能夠準確地評估浮游植物對營養鹽的反應，在本申請案中研發骨藻營養鹽相關指標基因的次世代定序平台，其結合導向定序及多重目標增殖策略充分利用次世代定序大量定序功能，藉此同時偵測分析不同骨藻種類對於環境氮、磷及矽營養鹽改變之基因反應，為其在藻華形成及族群群聚改變提供不同種類調適營養鹽變化之直接證據，希望將此一平台用以長時間高密度採樣來探討過去經常出現骨藻藻華之樣站，其中包括在馬祖沿岸夜光蟲觀測站，與及移地實驗至美國東岸著名的Narragansett Bay長期浮游生物觀測站，預期所獲得之結果可在實驗室實驗中比較不同骨藻種類如何調控營養鹽相關基因來適應不同環境之改變情形，應用在長期觀測站則有機會監測到在自然環境中不同骨藻種類因應環境變化的演替情形，這些浮游植物的調控機制也有助於探討當環境變遷時不同浮游植物種類因應的情形。Diatom generate 20 % of ocean primary production and play a central role in oceanic ecosystem. Skeletonema is known to form large diatom blooms in coastal and upwelling regions where nutrient-loading events usually act to stimulate blooms. With the advances in molecular biological techniques, molecular markers were proposed as powerful indicators to detect the physiological status of phytoplankton in response to environmental changes. In this proposal, we plan to develop a Skeletonema Nutrient-related Biomarker Sequencing Platform which is an NGS-based sequencing platform combined with targeted RNA sequencing and multiplex amplicon sequencing. It will enable efficient, quantitative multiplexed gene expression profiling for multiple nutrient related genes and time-sequential samples. This platform will be applied to conduct time-sequential profiling on natural assemblage in 2 Skeletonema bloom stations including Jie-Shou Au in the Nangan Island and the Long-Term Plankton Time Series Station in Narragansett Bay. This project will not only provide direct information to evaluate the physiological status in different Skeletonema species, but also contribute to explain the succession of Skeletonema blooms in response to environmental changes.

Keyword(s)

矽藻
骨藻
營養鹽相關指標基因
次世代定序
導向定序
Diatom
Skeletonema
nutrient-related biomarker
next generation sequencing
targeted RNA sequencing
multiplex amplicon sequencing

DSpace CRIS

High-Throughput Rna Sequencing Identifies the Transcriptional Responses of Skeletonema to Varying Nutrient Availability during the Formation of Blooms(I)

基本資料

Description