Molecular Cloning, Characterization and Application of Tyrosninase Gene Promoter in Ornamental Fish

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Project title

Code/計畫編號

94農科-14.2.4-漁-F1(2)

Translated Name/計畫中文名

觀賞魚色素調控基因tyrosinase酪胺酸脢基因啟動子選殖、特性之研究與應用

Project Coordinator/計畫主持人

Jenn-Kan Lu

Funding Organization/主管機關

Council of Agriculture,Executive Yuan

Department/Unit

Department of Aquaculture

Website

https://www.grb.gov.tw/search/planDetail?id=1157861

Year

2005

Start date/計畫起

01-01-2005

Expected Completion/計畫迄

31-12-2005

Bugetid/研究經費

600千元

ResearchField/研究領域

生物技術(農)
漁業

本年度目標將以分子生物技術選殖色素調控因子酪胺酸酶基因與其特性之研究，並將以基因轉殖技術生產不同體色素之轉基因觀賞魚利用基因轉殖、分子生物、基因工程與育種技術生產改良觀賞魚（包括：顏色，體型、外觀）其效益是多層的：
（一）建立獨特品系，使我國觀賞魚產業更具國際競爭力，增加觀賞魚之附加價值,提高利潤。
（二）增加對基因表現之調控機制在低等脊椎動物之了解，進而操控基因表現。
以基因工程快速生產表現型﹙phenotype﹚改變(modification)之觀賞魚新品種，增加台灣觀賞魚產業之競爭力。目前包括：歐、美、日本、中國大陸、新加坡、台灣均有轉基因魚、蝦、貝類與海藻之研究．台灣應積極部屬發展基因工程改良觀賞魚品種，以期趕上歐、美各國日形擴大的技術差距，方能使台灣在21世紀－生物科技時代中佔有一席之地，確保台灣日後的觀賞魚生物科技之競爭力與經濟利益 Desirable strains (e.g. coloration, emission of various fluorescent light, larger size, various phenotypical changes) would help Taiwan ornamental fish producers compete with foreign sources. Tyrosinase is the most important key enzyme in the pathway of melanin metabolism. The lacks of tyrosinase activity cause albinism. In the present study, it has been demonstrate that the tyrosinase effect in melanin synthesis. Employed reverse transcription polymerase chain reaction (RT-PCR) and RACE technique to amplify tyrosinase gene from the goldfish (Carassius auratus). Whole mount in situ hybridization analysis will be applied to detect the expression pattern of tyrosinase gene in early embryonic stage. In this study, the small interference RNA (siRNA) construct will also employed in observe the effect of inhibiting zebrafish tyrosinase gene.

Keyword(s)

酪胺酸酶
色素形成
小片段干擾核醣核酸
Tyrosinase
Pigmentation
Small Interference RNA

DSpace CRIS

Molecular Cloning, Characterization and Application of Tyrosninase Gene Promoter in Ornamental Fish

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