Establishment of Growth and Metamorphosis Related Genes as Molecular Markers of Breeding to Improve Broodstock of the Grouper

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Project title

Code/計畫編號

NSC99-2324-B019-004-MY2

Translated Name/計畫中文名

建立生長及變態相關基因分子標誌以進行石斑魚優良品種選育

Project Coordinator/計畫主持人

Hong-Yi Gong

Funding Organization/主管機關

National Science and Technology Council

Co-Investigator(s)/共同執行人

洪健睿

Department/Unit

Department of Aquaculture

Website

https://www.grb.gov.tw/search/planDetail?id=2222736

Year

2011

Start date/計畫起

01-08-2011

Expected Completion/計畫迄

31-07-2012

Bugetid/研究經費

1200千元

ResearchField/研究領域

生物技術(農)
漁業

"本計畫擬研究龍膽石斑生長時期及魚苗發育的關鍵變態時期表現之重要功能性基因，建立龍膽石斑生長及變態相關功能性基因資料庫及晶片，以做為標誌輔助型選種之分子標誌進行石斑魚優良品種之選育。本計畫預計工作項目如下： 􀁺 建構龍膽石斑生長相關重要組織（腦、肝臟及肌肉）及變態時期前後之cDNA library，以建立龍膽石斑生長與變態時期表現功能性基因EST及全長cDNA資料庫。 􀁺 整合cDNA library及PCR Subtraction Hybridization找到之龍膽石斑生長及變態相關功能性基因cDNAs，以建立吳郭魚生長相關功能性基因生物晶片之經驗，來建立龍膽石斑生長及變態相關功能性基因cDNAs之生物晶片。 􀁺 以此晶片尋找龍膽石斑稚魚以生長激素或重要生長因子處理，及以甲狀腺激素處理後參與調控之關鍵基因組，以做為石斑魚優良品種選育之重要分子標誌。 􀁺 將參與龍膽石斑稚魚生長之重要關鍵基因，以肌肉或肝臟組織專一性起動子建立基因轉殖斑馬魚，來驗證其在魚體生長的功能，並進一步以斑馬魚全轉錄體基因表現微晶片來探討其作用分子機制。 􀁺 分析龍膽石斑變態時期之細胞死亡、自我吞噬與先天免疫等相關基因。 􀁺 探討龍膽石斑變態時期被betanodavirus感染時其感受性如何，其體內之與細胞死亡、自我吞噬與先天免疫等控制相關基因變化及機制。" "In this project we would like to identify important functional genes during the growth period and metamorphosis stage of giant grouper (Epinephelus lanceoratus). According to giant grouper cDNA database and biochip established in this project, some growth and metamorphosis critical functional genes could be used as molecular markers of marker-assistant selection for breeding to improve broodstock of the grouper. The important items of this project were listed: 1. Establishment of giant grouper database of expression sequence tag (EST) and fill-length cDNAs of functional genes of growth and metamorphosis from cDNA libraries of growth-related important oragns (including brain, liver and muscle) and metamorphosis stages. 2. Establishment of giant grouper biochip representing cDNAs of functional genes of growth and metamorphosis by combination of information from cDNA library and PCR Subtraction Hybridization according to the experience of tilapia growth-related functional gene biochip. 3. By using the giant grouper growth and metamorphosis functional gene biochip, gene set involved in specific growth factor-induced growth response after treatment of juvenile giant grouper with growth hormone and important growth factors or gene set involved in thyroid hormone-induced metamorphosis after treatment of thyroid hormone will be identified. These critical gene set will be useful as molecular markers of selective breeding of grouper. 4. To verify the growth promotion function by establishment of transgenic zebrafish lines which overexpress growth-related functional gene candidates by liver- or muscle-specific promoter. By using zebrafish transcriptome gene expression microarray, genes up-regulated or down-regualted by grouper growth-promotion gene in transgenic zebrafish will be analyzed to study the molecular mechanism of specific growth-promotion gene. 5. Analysis of programmed cell death, autophagy and innate immunity response related genes during metamorphosis of giant grouper. 6. To study the suscesibility of grouper infected by betanodavirus during metamorphosis and mechanism of how the betanodavirus affects programmed cell death, autophagy and innate immunity response related genes during metamorphosis."

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Establishment of Growth and Metamorphosis Related Genes as Molecular Markers of Breeding to Improve Broodstock of the Grouper

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