Molecular Mechanism of Gonadal Development and Puberty in Grouper

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Project title

Code/計畫編號

NSC99-2321-B019-006-MY3

Translated Name/計畫中文名

石斑魚生殖腺性別分化與發育及青春期啟動機制之研究

Project Coordinator/計畫主持人

Ching-Fong Chang

Funding Organization/主管機關

National Science and Technology Council

Department/Unit

Department of Aquaculture

Website

https://www.grb.gov.tw/search/planDetail?id=2103273

Year

2010

Start date/計畫起

01-08-2011

Expected Completion/計畫迄

31-07-2012

Bugetid/研究經費

2500千元

ResearchField/研究領域

漁業
生物技術(農)

"石斑魚目前是台灣最重要與高經濟的養殖魚種，點帶石斑主要先發育為雌性，3~4年齡進入青春期，約7年齡性轉變為雄性，因此是屬於先雌後雄的性轉變魚種，我們認為點帶石斑是很適合做為雌性的性別分化及生殖腺發育之研究模式動物。有關動物的雌性性別分化與啟動青春期的機制仍然十分不清楚。因此本研究計畫擬以點帶石斑為模式研究魚種，利用組織學、內分泌、細胞生物及分子遺傳等策略，整合性的探討點帶石斑在生殖腺性別分化及青春期啟動的分子機制，預期本研究成果除了瞭解點帶石斑的性別分化與青春期的機制外，並可應用於其他種類的石斑魚，對石斑魚的養殖產業有應用的可能性。本計畫以三年期，分別進行下列之實驗。 1. 瞭解點帶石斑性別分化之生殖腺細胞及組織的特性。 2. 以抗體免疫染色，瞭解點帶石斑性別分化時生殖腺Cyp19a1a（aromatase）酵素蛋白表現。 3. 瞭解點帶石斑性別分化時，生殖腺相關基因（wnt4，follistatin，foxl2，figα與cyp19a1a）等基因表現之關係。 4. 利用aromatase inhibitor，瞭解內生性雌二醇（estradiol）在點帶石斑性別分化的重要性。 5. 利用甲基化睪固酮（17α-methyltestesterone），瞭解雄性類固醇激素對點帶石斑的生殖腺發育及相關雌性分化基因及雄性基因（dmrt1）之表現影響。 6. 瞭解雌二醇與睪固酮對點帶石斑雌性基因（wnt4、follistatin、foxl2、cyp19a1a與figα）與雄性基因（dmrt1）基因表現之影響。 7. 瞭解腦-下視丘系統（kisspeptins-GnRH或是dopamine-GnRH）在點帶石斑的生殖腺發育及青春期啟動的可能角色。 8. 瞭解腦下垂體（gonadotropins）對點帶石斑青春期啟動的可能角色。 9. 以外源性GnRH（利用長效性LHRH analog）及pimozide（dopamine的拮抗物），瞭解對啟動點帶石斑進入青春期的影響。" "Orange-spotted grouper (Epinephelus coioides) a marine protogynous hermaphrodite, is differentiated to female first, reached to puberty at the age of 3 to 4 years. Then the fish change to male at 7 years of age. Orange-spotted grouper is considered as a good model fish to study female sex differentiation and gonadal growth. There is still not much clear in the female sex differentiation and puberty in grouper and other fish species. Therefore, the objectives are to investigate the molecular mechanism of female gonad sex differentiation and puberty in the orange-spotted grouper. We will choose the female genes (wnt4, follistatin, foxl2, cyp19a1a, figα) and male gene (dmrt1) to conduct. The experiments will include as follows: 1. The histological characters in gonadal tissue during female sex differentiation in orange-spotted grouper. 2. The expression profiles and localization of aromatase in the gonad during gonadal sex differentiation. 3. The expression profiles of the female genes (wnt4, follistatin, foxl2, cyp19a1a, figα) in the gonad during sex differentiation. 4. The effects of aromatase inhibitor in the female gonadal sex differentiation and gene expression of wnt4, follistatin, foxl2, cyp19a1a and figα. 5. The effects of 17α-methyltestosterone in the gonadal sex differentiation and gene expression of female genes (wnt4, follistatin, foxl2, cyp19a1a, figα) and male gene (dmrt1). 6. The possible roles of brain related to kisspeptins-GnRH and dopamine in the development of puberty. 7. The possible roles of pituitary (GnRH receptor, dopamine receptor, FSHβ, LHβ and GtHα) in the development of puberty. 8. The effects of “LHRH analog” and “LHRH analog + pimozide” in the development of puberty and pituitary gonadotropins."

Keyword(s)

Aromatase
腦發育
cyp19a1b
石斑
生殖腺分化
Neurosteroidogenesis

DSpace CRIS

Molecular Mechanism of Gonadal Development and Puberty in Grouper

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