Rapid Disease Detection and Diagnosis for Aquacultured Fish Using Atomic Force Microscope

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Project title

Code/計畫編號

92農科-4.2.3-漁-F1(11)

Translated Name/計畫中文名

應用奈米檢測技術於養殖水產生物疾病之快速檢測及診斷

Project Coordinator/計畫主持人

Jyh-Jong Sheen

Funding Organization/主管機關

Council of Agriculture,Executive Yuan

Co-Investigator(s)/共同執行人

林世明
謝哲偉

Department/Unit

Department of Mechanical and Mechatronic Engineering

Website

https://www.grb.gov.tw/search/planDetail?id=960827

Year

2003

Start date/計畫起

01-01-2003

Expected Completion/計畫迄

01-12-2003

Bugetid/研究經費

700千元

ResearchField/研究領域

漁業

原子力顯微鏡由於具有原子辨識的極佳影像解析度、不需樣品之實驗前準備、以及可在液相中操作的能力的優點,非常適合生物樣本的觀測.我們將應用原子力顯微鏡於養殖水產生物疾病之快速檢測.相較於一般檢測抗體-抗原鍵結常用的方法如免疫螢光法、免疫燐光法及放射線免疫偵測法,原子力顯微鏡可區別單一抗體、抗原或鍵結配對的抗體-抗原的辨識能力,而且檢測快速(只需數分鐘),其解析能力非一般方法可以相比.本計畫目標為結合本校水產養殖系、微機電系統實驗室、台灣大學生物光電醫學中心,將原子力顯微鏡的奈米檢測技術應用於養殖水產生物疾病(例如海鱺巴斯德氏桿菌)之快速檢測. Atomic force microscope (AFM) has been used increasingly during the past few years to inverstigate biomolecular structures at nanometer scale resolution. The technique provides three-dimensional images of the surface topology with molecular resolution, in real time, under physiological conditions, abd with minimal sample preparation. AFM can also be used to probe the physical properties of the specimen, such as molecular interactions, surfce hydrophobicity, and mechanical properties. The goal of this study is to use AFM as a biosensor to detect specific interation between antigens and antibodies. Compared to traditional detection techniques for antigen-antibody interaction, the detection time and resolution will be improved substantially. Therefore, AFM will be utilized as a tool for rapid disease detection and diagnosis for aquacultured fish.

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Rapid Disease Detection and Diagnosis for Aquacultured Fish Using Atomic Force Microscope

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